Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5263 and Pf1N1B4_5264 are separated by 10 nucleotides Pf1N1B4_5264 and Pf1N1B4_5265 are separated by 299 nucleotides Pf1N1B4_5265 and Pf1N1B4_5266 are separated by 21 nucleotides
Pf1N1B4_5263 - hypothetical protein, at 22,526 to 22,723
_5263
Pf1N1B4_5264 - hypothetical protein, at 22,734 to 22,868
_5264
Pf1N1B4_5265 - hypothetical protein, at 23,168 to 23,296
_5265
Pf1N1B4_5266 - hypothetical protein, at 23,318 to 23,638
_5266
Position (kb)
23
24 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 22.527 kb on + strand at 22.528 kb on - strand at 22.541 kb on + strand at 22.541 kb on + strand at 22.542 kb on - strand at 22.542 kb on - strand at 22.542 kb on - strand at 22.542 kb on - strand at 22.604 kb on + strand, within Pf1N1B4_5263 at 22.604 kb on + strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.670 kb on + strand, within Pf1N1B4_5263 at 22.671 kb on - strand, within Pf1N1B4_5263 at 22.671 kb on - strand at 22.671 kb on - strand at 22.671 kb on - strand, within Pf1N1B4_5263 at 22.718 kb on - strand at 22.718 kb on - strand at 22.797 kb on + strand, within Pf1N1B4_5264 at 22.798 kb on - strand at 22.878 kb on + strand at 22.879 kb on - strand at 22.879 kb on - strand at 22.905 kb on - strand at 22.906 kb on + strand at 22.907 kb on - strand at 22.907 kb on - strand at 22.938 kb on - strand at 22.958 kb on - strand at 22.974 kb on - strand at 23.042 kb on - strand at 23.042 kb on - strand at 23.042 kb on - strand at 23.042 kb on - strand at 23.042 kb on - strand at 23.077 kb on - strand at 23.077 kb on - strand at 23.211 kb on - strand, within Pf1N1B4_5265 at 23.211 kb on - strand, within Pf1N1B4_5265 at 23.211 kb on - strand, within Pf1N1B4_5265 at 23.251 kb on + strand, within Pf1N1B4_5265 at 23.252 kb on - strand, within Pf1N1B4_5265 at 23.252 kb on - strand, within Pf1N1B4_5265 at 23.252 kb on - strand, within Pf1N1B4_5265 at 23.252 kb on - strand, within Pf1N1B4_5265 at 23.252 kb on - strand, within Pf1N1B4_5265 at 23.312 kb on + strand at 23.312 kb on + strand at 23.313 kb on - strand at 23.313 kb on - strand at 23.398 kb on + strand, within Pf1N1B4_5266 at 23.399 kb on - strand, within Pf1N1B4_5266 at 23.533 kb on - strand, within Pf1N1B4_5266 at 23.533 kb on - strand, within Pf1N1B4_5266 at 23.686 kb on + strand at 23.687 kb on - strand at 23.693 kb on - strand at 23.810 kb on + strand at 23.840 kb on + strand at 23.840 kb on + strand at 23.841 kb on - strand at 23.841 kb on - strand at 23.841 kb on - strand at 23.932 kb on + strand at 23.942 kb on + strand at 23.942 kb on + strand at 23.942 kb on + strand at 24.094 kb on + strand at 24.094 kb on + strand at 24.094 kb on + strand at 24.156 kb on + strand at 24.292 kb on + strand at 24.292 kb on + strand at 24.293 kb on - strand at 24.293 kb on - strand at 24.293 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 22,527 + -0.0 22,528 - -0.7 22,541 + -0.0 22,541 + -0.0 22,542 - -0.0 22,542 - -0.0 22,542 - -0.0 22,542 - -0.0 22,604 + Pf1N1B4_5263 0.39 -1.8 22,604 + Pf1N1B4_5263 0.39 -2.0 22,605 - Pf1N1B4_5263 0.40 -2.4 22,605 - Pf1N1B4_5263 0.40 -2.1 22,605 - Pf1N1B4_5263 0.40 -0.0 22,605 - Pf1N1B4_5263 0.40 -2.3 22,605 - Pf1N1B4_5263 0.40 -1.3 22,605 - Pf1N1B4_5263 0.40 -2.4 22,605 - Pf1N1B4_5263 0.40 -0.0 22,605 - Pf1N1B4_5263 0.40 -0.0 22,605 - Pf1N1B4_5263 0.40 -0.0 22,605 - Pf1N1B4_5263 0.40 -0.0 22,670 + Pf1N1B4_5263 0.73 -1.0 22,671 - Pf1N1B4_5263 0.73 -0.0 22,671 - -1.8 22,671 - -0.0 22,671 - Pf1N1B4_5263 0.73 -2.2 22,718 - -1.4 22,718 - -1.7 22,797 + Pf1N1B4_5264 0.47 -0.0 22,798 - -0.0 22,878 + -1.3 22,879 - -0.0 22,879 - -0.0 22,905 - -1.9 22,906 + -0.7 22,907 - -0.0 22,907 - -2.2 22,938 - -0.0 22,958 - -0.0 22,974 - -2.9 23,042 - -1.2 23,042 - -0.0 23,042 - -2.9 23,042 - -2.9 23,042 - -0.0 23,077 - -0.0 23,077 - -0.0 23,211 - Pf1N1B4_5265 0.33 -0.0 23,211 - Pf1N1B4_5265 0.33 -0.0 23,211 - Pf1N1B4_5265 0.33 -0.0 23,251 + Pf1N1B4_5265 0.64 -0.0 23,252 - Pf1N1B4_5265 0.65 -0.0 23,252 - Pf1N1B4_5265 0.65 -2.6 23,252 - Pf1N1B4_5265 0.65 -0.0 23,252 - Pf1N1B4_5265 0.65 -3.6 23,252 - Pf1N1B4_5265 0.65 -0.0 23,312 + -0.8 23,312 + -0.0 23,313 - -2.0 23,313 - -0.0 23,398 + Pf1N1B4_5266 0.25 -0.0 23,399 - Pf1N1B4_5266 0.25 -0.0 23,533 - Pf1N1B4_5266 0.67 -0.0 23,533 - Pf1N1B4_5266 0.67 -0.0 23,686 + -0.0 23,687 - -2.2 23,693 - -2.1 23,810 + -3.0 23,840 + -0.0 23,840 + -0.0 23,841 - -0.0 23,841 - -0.0 23,841 - -2.0 23,932 + -0.0 23,942 + -0.6 23,942 + -0.4 23,942 + -1.9 24,094 + -2.8 24,094 + -0.0 24,094 + -1.6 24,156 + -0.0 24,292 + -0.0 24,292 + -0.0 24,293 - -0.0 24,293 - -0.0 24,293 - -3.1
Or see this region's nucleotide sequence