Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5239 and Pf1N1B4_5240 are separated by 173 nucleotides Pf1N1B4_5240 and Pf1N1B4_5241 are separated by 198 nucleotides Pf1N1B4_5241 and Pf1N1B4_5242 overlap by 8 nucleotides
Pf1N1B4_5239 - hypothetical protein, at 184 to 579
_5239
Pf1N1B4_5240 - hypothetical protein, at 753 to 872
_5240
Pf1N1B4_5241 - hypothetical protein, at 1,071 to 1,271
_5241
Pf1N1B4_5242 - hypothetical protein, at 1,264 to 1,524
_5242
Position (kb) 0
1 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 0.011 kb on + strand at 0.011 kb on + strand at 0.011 kb on + strand at 0.040 kb on + strand at 0.040 kb on + strand at 0.040 kb on + strand at 0.041 kb on - strand at 0.124 kb on + strand at 0.124 kb on + strand at 0.125 kb on - strand at 0.125 kb on - strand at 0.125 kb on - strand at 0.146 kb on - strand at 0.186 kb on - strand at 0.284 kb on + strand, within Pf1N1B4_5239 at 0.285 kb on - strand, within Pf1N1B4_5239 at 0.463 kb on - strand, within Pf1N1B4_5239 at 0.494 kb on + strand, within Pf1N1B4_5239 at 0.494 kb on + strand at 0.495 kb on - strand, within Pf1N1B4_5239 at 0.496 kb on + strand, within Pf1N1B4_5239 at 0.496 kb on + strand, within Pf1N1B4_5239 at 0.496 kb on + strand, within Pf1N1B4_5239 at 0.496 kb on + strand, within Pf1N1B4_5239 at 0.497 kb on - strand, within Pf1N1B4_5239 at 0.597 kb on - strand at 0.773 kb on - strand, within Pf1N1B4_5240 at 0.773 kb on - strand, within Pf1N1B4_5240 at 0.773 kb on - strand, within Pf1N1B4_5240 at 0.943 kb on + strand at 0.943 kb on + strand at 0.992 kb on + strand at 1.074 kb on + strand at 1.074 kb on + strand at 1.074 kb on + strand at 1.074 kb on + strand at 1.074 kb on + strand at 1.075 kb on - strand at 1.075 kb on - strand at 1.092 kb on - strand, within Pf1N1B4_5241 at 1.265 kb on + strand at 1.265 kb on + strand at 1.337 kb on - strand, within Pf1N1B4_5242 at 1.369 kb on + strand, within Pf1N1B4_5242 at 1.407 kb on + strand, within Pf1N1B4_5242 at 1.407 kb on + strand, within Pf1N1B4_5242 at 1.501 kb on + strand at 1.501 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 11 + -1.7 11 + -0.0 11 + -0.0 40 + -1.1 40 + -0.0 40 + -1.2 41 - -0.0 124 + -0.0 124 + -1.5 125 - -1.8 125 - -0.0 125 - -0.0 146 - -1.8 186 - -2.8 284 + Pf1N1B4_5239 0.25 -2.9 285 - Pf1N1B4_5239 0.26 -0.0 463 - Pf1N1B4_5239 0.70 -2.6 494 + Pf1N1B4_5239 0.78 -0.0 494 + -1.9 495 - Pf1N1B4_5239 0.79 -0.0 496 + Pf1N1B4_5239 0.79 -0.0 496 + Pf1N1B4_5239 0.79 -0.0 496 + Pf1N1B4_5239 0.79 -0.0 496 + Pf1N1B4_5239 0.79 -1.9 497 - Pf1N1B4_5239 0.79 -0.0 597 - -1.0 773 - Pf1N1B4_5240 0.17 -0.5 773 - Pf1N1B4_5240 0.17 +0.5 773 - Pf1N1B4_5240 0.17 -0.0 943 + -1.9 943 + -0.0 992 + -0.0 1,074 + -0.0 1,074 + -1.0 1,074 + -0.0 1,074 + -0.0 1,074 + -1.6 1,075 - -0.0 1,075 - -2.3 1,092 - Pf1N1B4_5241 0.10 -1.0 1,265 + -0.0 1,265 + -2.1 1,337 - Pf1N1B4_5242 0.28 -0.0 1,369 + Pf1N1B4_5242 0.40 -1.2 1,407 + Pf1N1B4_5242 0.55 -2.2 1,407 + Pf1N1B4_5242 0.55 -0.0 1,501 + -2.3 1,501 + -1.7
Or see this region's nucleotide sequence