Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3456 and Pf1N1B4_3457 overlap by 26 nucleotides Pf1N1B4_3457 and Pf1N1B4_3458 are separated by 23 nucleotides Pf1N1B4_3458 and Pf1N1B4_5224 are separated by 292 nucleotides Pf1N1B4_5224 and Pf1N1B4_3459 are separated by 86 nucleotides
Pf1N1B4_3456 - ATP-binding protein PhnN; Guanylate kinase (EC 2.7.4.8), at 3,853,874 to 3,854,422
_3456
Pf1N1B4_3457 - hypothetical protein, at 3,854,397 to 3,854,576
_3457
Pf1N1B4_3458 - hypothetical protein, at 3,854,600 to 3,854,833
_3458
Pf1N1B4_5224 - tRNA-Arg-CCT, at 3,855,126 to 3,855,199
_5224
Pf1N1B4_3459 - T1SS secreted agglutinin RTX, at 3,855,286 to 3,862,629
_3459
Position (kb)
3855
3856 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3854.188 kb on - strand, within Pf1N1B4_3456 at 3854.188 kb on - strand at 3854.191 kb on - strand, within Pf1N1B4_3456 at 3854.199 kb on + strand, within Pf1N1B4_3456 at 3854.239 kb on - strand, within Pf1N1B4_3456 at 3854.323 kb on + strand, within Pf1N1B4_3456 at 3854.323 kb on + strand, within Pf1N1B4_3456 at 3854.359 kb on + strand, within Pf1N1B4_3456 at 3854.360 kb on - strand, within Pf1N1B4_3456 at 3854.360 kb on - strand, within Pf1N1B4_3456 at 3854.360 kb on - strand, within Pf1N1B4_3456 at 3854.360 kb on - strand, within Pf1N1B4_3456 at 3854.364 kb on + strand, within Pf1N1B4_3456 at 3854.365 kb on - strand, within Pf1N1B4_3456 at 3854.570 kb on + strand at 3854.571 kb on - strand at 3854.571 kb on - strand at 3854.642 kb on - strand, within Pf1N1B4_3458 at 3854.642 kb on - strand at 3854.724 kb on + strand, within Pf1N1B4_3458 at 3854.725 kb on - strand, within Pf1N1B4_3458 at 3855.029 kb on + strand at 3855.029 kb on + strand at 3855.029 kb on + strand at 3855.029 kb on + strand at 3855.030 kb on - strand at 3855.035 kb on + strand at 3855.036 kb on - strand at 3855.036 kb on - strand at 3855.133 kb on + strand at 3855.146 kb on + strand, within Pf1N1B4_5224 at 3855.146 kb on + strand, within Pf1N1B4_5224 at 3855.147 kb on - strand, within Pf1N1B4_5224 at 3855.162 kb on + strand, within Pf1N1B4_5224 at 3855.162 kb on + strand, within Pf1N1B4_5224 at 3855.163 kb on - strand, within Pf1N1B4_5224 at 3855.205 kb on + strand at 3855.205 kb on + strand at 3855.205 kb on + strand at 3855.205 kb on + strand at 3855.205 kb on + strand at 3855.205 kb on + strand at 3855.206 kb on - strand at 3855.300 kb on + strand at 3855.300 kb on + strand at 3855.300 kb on + strand at 3855.300 kb on + strand at 3855.300 kb on + strand at 3855.301 kb on - strand at 3855.301 kb on - strand at 3855.301 kb on - strand at 3855.301 kb on - strand at 3855.387 kb on + strand at 3855.539 kb on + strand at 3855.540 kb on - strand at 3855.540 kb on - strand at 3855.578 kb on + strand at 3855.579 kb on - strand at 3855.579 kb on - strand at 3855.579 kb on - strand at 3855.579 kb on - strand at 3855.588 kb on + strand at 3855.589 kb on - strand at 3855.589 kb on - strand at 3855.589 kb on - strand at 3855.667 kb on + strand at 3855.667 kb on + strand at 3855.667 kb on + strand at 3855.667 kb on + strand at 3855.668 kb on - strand at 3855.668 kb on - strand at 3855.716 kb on + strand at 3855.717 kb on - strand at 3855.717 kb on - strand at 3855.717 kb on - strand at 3855.717 kb on - strand at 3855.770 kb on + strand at 3855.770 kb on + strand at 3855.771 kb on - strand at 3855.854 kb on + strand at 3855.855 kb on - strand at 3856.108 kb on + strand, within Pf1N1B4_3459 at 3856.109 kb on - strand, within Pf1N1B4_3459
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 3,854,188 - Pf1N1B4_3456 0.57 -2.4 3,854,188 - -0.0 3,854,191 - Pf1N1B4_3456 0.58 -1.8 3,854,199 + Pf1N1B4_3456 0.59 -0.0 3,854,239 - Pf1N1B4_3456 0.66 -0.0 3,854,323 + Pf1N1B4_3456 0.82 -2.3 3,854,323 + Pf1N1B4_3456 0.82 -0.0 3,854,359 + Pf1N1B4_3456 0.88 -0.0 3,854,360 - Pf1N1B4_3456 0.89 -0.0 3,854,360 - Pf1N1B4_3456 0.89 -1.2 3,854,360 - Pf1N1B4_3456 0.89 -1.6 3,854,360 - Pf1N1B4_3456 0.89 -0.4 3,854,364 + Pf1N1B4_3456 0.89 -1.7 3,854,365 - Pf1N1B4_3456 0.89 -1.2 3,854,570 + -0.0 3,854,571 - -0.9 3,854,571 - -3.3 3,854,642 - Pf1N1B4_3458 0.18 -0.0 3,854,642 - -2.6 3,854,724 + Pf1N1B4_3458 0.53 -1.4 3,854,725 - Pf1N1B4_3458 0.53 -0.0 3,855,029 + -0.7 3,855,029 + -0.4 3,855,029 + -2.4 3,855,029 + -0.0 3,855,030 - -1.3 3,855,035 + -0.0 3,855,036 - -1.9 3,855,036 - -1.7 3,855,133 + -1.8 3,855,146 + Pf1N1B4_5224 0.27 -0.0 3,855,146 + Pf1N1B4_5224 0.27 -3.3 3,855,147 - Pf1N1B4_5224 0.28 -1.6 3,855,162 + Pf1N1B4_5224 0.49 -1.7 3,855,162 + Pf1N1B4_5224 0.49 -0.0 3,855,163 - Pf1N1B4_5224 0.50 -2.0 3,855,205 + -0.7 3,855,205 + -0.0 3,855,205 + -0.0 3,855,205 + -0.0 3,855,205 + -0.0 3,855,205 + -2.5 3,855,206 - -0.0 3,855,300 + -1.8 3,855,300 + -0.0 3,855,300 + -0.0 3,855,300 + +0.6 3,855,300 + -1.7 3,855,301 - -0.0 3,855,301 - -0.0 3,855,301 - -0.0 3,855,301 - -0.0 3,855,387 + -1.4 3,855,539 + -0.0 3,855,540 - -0.0 3,855,540 - -0.0 3,855,578 + -2.0 3,855,579 - -2.1 3,855,579 - +0.1 3,855,579 - -0.0 3,855,579 - -0.0 3,855,588 + -0.0 3,855,589 - -0.0 3,855,589 - -0.0 3,855,589 - -0.0 3,855,667 + -0.0 3,855,667 + -1.9 3,855,667 + -2.4 3,855,667 + -1.9 3,855,668 - -1.0 3,855,668 - -0.0 3,855,716 + -2.3 3,855,717 - -0.0 3,855,717 - -1.7 3,855,717 - -0.0 3,855,717 - -1.5 3,855,770 + -2.1 3,855,770 + -0.0 3,855,771 - -0.0 3,855,854 + -0.0 3,855,855 - -0.0 3,856,108 + Pf1N1B4_3459 0.11 -2.6 3,856,109 - Pf1N1B4_3459 0.11 -2.4
Or see this region's nucleotide sequence