Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_515 and Pf1N1B4_516 overlap by 1 nucleotides Pf1N1B4_516 and Pf1N1B4_517 are separated by 80 nucleotides Pf1N1B4_517 and Pf1N1B4_518 are separated by 12 nucleotides
Pf1N1B4_515 - DNA-binding response regulator GltR, controls specific porins for the entry of glucose, at 606,483 to 607,241
_515
Pf1N1B4_516 - Sensor histidine kinase, at 607,241 to 608,293
_516
Pf1N1B4_517 - hypothetical protein, at 608,374 to 608,517
_517
Pf1N1B4_518 - FKBP-type peptidyl-prolyl cis-trans isomerase FkpA precursor (EC 5.2.1.8), at 608,530 to 609,378
_518
Position (kb) 607
608
609 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 606.294 kb on + strand at 606.294 kb on + strand at 606.295 kb on - strand at 606.415 kb on + strand at 606.416 kb on - strand at 606.441 kb on + strand at 606.441 kb on + strand at 606.441 kb on + strand at 606.442 kb on - strand at 606.461 kb on + strand at 606.461 kb on + strand at 606.461 kb on + strand at 606.536 kb on + strand at 606.604 kb on - strand, within Pf1N1B4_515 at 606.621 kb on - strand, within Pf1N1B4_515 at 606.837 kb on + strand, within Pf1N1B4_515 at 606.837 kb on + strand, within Pf1N1B4_515 at 606.837 kb on + strand, within Pf1N1B4_515 at 606.837 kb on + strand, within Pf1N1B4_515 at 606.838 kb on - strand, within Pf1N1B4_515 at 606.838 kb on - strand, within Pf1N1B4_515 at 606.838 kb on - strand at 606.838 kb on - strand, within Pf1N1B4_515 at 606.838 kb on - strand, within Pf1N1B4_515 at 606.955 kb on + strand at 607.213 kb on - strand at 607.213 kb on - strand at 607.258 kb on + strand at 607.302 kb on + strand at 607.302 kb on + strand at 607.303 kb on - strand at 607.832 kb on + strand, within Pf1N1B4_516 at 607.832 kb on + strand, within Pf1N1B4_516 at 607.832 kb on + strand at 607.832 kb on + strand, within Pf1N1B4_516 at 607.833 kb on - strand at 607.833 kb on - strand, within Pf1N1B4_516 at 608.158 kb on + strand, within Pf1N1B4_516 at 608.158 kb on + strand, within Pf1N1B4_516 at 608.407 kb on - strand, within Pf1N1B4_517 at 608.455 kb on - strand, within Pf1N1B4_517 at 608.486 kb on - strand at 608.486 kb on - strand, within Pf1N1B4_517 at 608.486 kb on - strand, within Pf1N1B4_517 at 608.486 kb on - strand at 608.554 kb on + strand at 608.554 kb on + strand at 608.555 kb on - strand at 608.555 kb on - strand at 608.563 kb on + strand at 608.563 kb on + strand at 608.563 kb on + strand at 608.564 kb on - strand at 608.564 kb on - strand at 608.564 kb on - strand at 608.564 kb on - strand at 608.564 kb on - strand at 608.565 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 606,294 + -2.4 606,294 + -1.1 606,295 - -1.5 606,415 + -0.0 606,416 - -0.0 606,441 + -1.1 606,441 + -1.4 606,441 + -0.0 606,442 - -0.0 606,461 + -0.0 606,461 + -2.4 606,461 + -0.0 606,536 + -0.0 606,604 - Pf1N1B4_515 0.16 -0.0 606,621 - Pf1N1B4_515 0.18 -0.0 606,837 + Pf1N1B4_515 0.47 -1.0 606,837 + Pf1N1B4_515 0.47 -0.0 606,837 + Pf1N1B4_515 0.47 -1.3 606,837 + Pf1N1B4_515 0.47 -0.0 606,838 - Pf1N1B4_515 0.47 -0.0 606,838 - Pf1N1B4_515 0.47 -0.0 606,838 - -0.0 606,838 - Pf1N1B4_515 0.47 -3.5 606,838 - Pf1N1B4_515 0.47 -0.0 606,955 + -0.0 607,213 - -2.6 607,213 - -1.9 607,258 + -0.0 607,302 + -0.0 607,302 + -0.0 607,303 - -1.7 607,832 + Pf1N1B4_516 0.56 -0.0 607,832 + Pf1N1B4_516 0.56 -0.0 607,832 + -0.0 607,832 + Pf1N1B4_516 0.56 -2.5 607,833 - -1.6 607,833 - Pf1N1B4_516 0.56 -0.0 608,158 + Pf1N1B4_516 0.87 -0.0 608,158 + Pf1N1B4_516 0.87 -0.0 608,407 - Pf1N1B4_517 0.23 -2.5 608,455 - Pf1N1B4_517 0.56 -0.0 608,486 - -0.0 608,486 - Pf1N1B4_517 0.78 -0.0 608,486 - Pf1N1B4_517 0.78 -0.0 608,486 - -0.0 608,554 + -0.0 608,554 + +1.3 608,555 - -3.0 608,555 - -0.0 608,563 + -1.4 608,563 + -0.0 608,563 + -0.0 608,564 - -0.0 608,564 - -0.7 608,564 - -1.0 608,564 - -1.0 608,564 - -0.0 608,565 + -0.0
Or see this region's nucleotide sequence