Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5127

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5126 and Pf1N1B4_5127 overlap by 20 nucleotidesPf1N1B4_5127 and Pf1N1B4_5128 are separated by 38 nucleotides Pf1N1B4_5126 - HlyD family secretion protein, at 5,702,940 to 5,704,253 _5126 Pf1N1B4_5127 - ABC-type protease/lipase transport system, ATPase and permease components, at 5,704,234 to 5,705,214 _5127 Pf1N1B4_5128 - ABC-type protease/lipase transport system, ATPase and permease components, at 5,705,253 to 5,706,014 _5128 Position (kb) 5704 5705 5706Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 5703.395 kb on + strand, within Pf1N1B4_5126at 5703.442 kb on + strand, within Pf1N1B4_5126at 5703.442 kb on + strand, within Pf1N1B4_5126at 5703.443 kb on - strand, within Pf1N1B4_5126at 5703.510 kb on + strand, within Pf1N1B4_5126at 5703.510 kb on + strand, within Pf1N1B4_5126at 5703.511 kb on - strand, within Pf1N1B4_5126at 5703.554 kb on + strand, within Pf1N1B4_5126at 5703.807 kb on + strand, within Pf1N1B4_5126at 5703.807 kb on + strand, within Pf1N1B4_5126at 5703.861 kb on + strand, within Pf1N1B4_5126at 5703.959 kb on - strandat 5704.036 kb on - strand, within Pf1N1B4_5126at 5704.173 kb on + strandat 5704.173 kb on + strandat 5704.173 kb on + strandat 5704.212 kb on + strandat 5704.256 kb on + strandat 5704.257 kb on - strandat 5704.421 kb on - strand, within Pf1N1B4_5127at 5704.421 kb on - strand, within Pf1N1B4_5127at 5704.552 kb on - strandat 5704.791 kb on - strandat 5704.795 kb on + strand, within Pf1N1B4_5127at 5704.796 kb on - strandat 5704.796 kb on - strandat 5704.796 kb on - strand, within Pf1N1B4_5127at 5704.796 kb on - strand, within Pf1N1B4_5127at 5704.799 kb on + strand, within Pf1N1B4_5127at 5704.799 kb on + strand, within Pf1N1B4_5127at 5704.800 kb on - strandat 5704.800 kb on - strand, within Pf1N1B4_5127at 5704.800 kb on - strand, within Pf1N1B4_5127at 5705.055 kb on + strandat 5705.055 kb on + strand, within Pf1N1B4_5127at 5705.055 kb on + strand, within Pf1N1B4_5127at 5705.055 kb on + strand, within Pf1N1B4_5127at 5705.055 kb on + strand, within Pf1N1B4_5127at 5705.055 kb on + strand, within Pf1N1B4_5127at 5705.055 kb on + strand, within Pf1N1B4_5127at 5705.056 kb on - strand, within Pf1N1B4_5127at 5705.056 kb on - strand, within Pf1N1B4_5127at 5705.234 kb on + strandat 5705.248 kb on + strandat 5705.249 kb on - strandat 5705.249 kb on - strandat 5705.432 kb on - strand, within Pf1N1B4_5128at 5705.432 kb on - strand, within Pf1N1B4_5128at 5705.581 kb on + strand, within Pf1N1B4_5128at 5705.581 kb on + strand, within Pf1N1B4_5128at 5705.581 kb on + strand, within Pf1N1B4_5128at 5705.582 kb on - strand, within Pf1N1B4_5128at 5705.582 kb on - strandat 5705.586 kb on - strand, within Pf1N1B4_5128at 5705.723 kb on - strand, within Pf1N1B4_5128at 5705.757 kb on - strand, within Pf1N1B4_5128at 5705.882 kb on - strand, within Pf1N1B4_5128at 5705.894 kb on + strand, within Pf1N1B4_5128at 5705.894 kb on + strand, within Pf1N1B4_5128at 5705.895 kb on - strandat 5705.896 kb on + strand, within Pf1N1B4_5128at 5705.896 kb on + strand, within Pf1N1B4_5128at 5705.896 kb on + strand, within Pf1N1B4_5128at 5705.896 kb on + strand, within Pf1N1B4_5128at 5705.896 kb on + strandat 5705.897 kb on - strand, within Pf1N1B4_5128at 5705.897 kb on - strand, within Pf1N1B4_5128at 5705.897 kb on - strand, within Pf1N1B4_5128at 5705.897 kb on - strand, within Pf1N1B4_5128at 5705.897 kb on - strand, within Pf1N1B4_5128at 5705.897 kb on - strand, within Pf1N1B4_5128at 5705.954 kb on + strandat 5706.051 kb on + strandat 5706.052 kb on - strandat 5706.158 kb on + strandat 5706.158 kb on + strandat 5706.158 kb on + strandat 5706.158 kb on + strandat 5706.159 kb on - strandat 5706.159 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
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5,703,395 + Pf1N1B4_5126 0.35 -1.8
5,703,442 + Pf1N1B4_5126 0.38 -0.0
5,703,442 + Pf1N1B4_5126 0.38 -0.0
5,703,443 - Pf1N1B4_5126 0.38 -1.8
5,703,510 + Pf1N1B4_5126 0.43 -0.0
5,703,510 + Pf1N1B4_5126 0.43 -3.4
5,703,511 - Pf1N1B4_5126 0.43 -2.7
5,703,554 + Pf1N1B4_5126 0.47 -0.0
5,703,807 + Pf1N1B4_5126 0.66 -0.0
5,703,807 + Pf1N1B4_5126 0.66 -0.0
5,703,861 + Pf1N1B4_5126 0.70 -0.0
5,703,959 - -0.0
5,704,036 - Pf1N1B4_5126 0.83 -0.0
5,704,173 + -1.8
5,704,173 + -0.0
5,704,173 + -2.4
5,704,212 + -0.0
5,704,256 + -0.0
5,704,257 - -1.7
5,704,421 - Pf1N1B4_5127 0.19 -0.0
5,704,421 - Pf1N1B4_5127 0.19 -0.0
5,704,552 - -3.4
5,704,791 - -0.7
5,704,795 + Pf1N1B4_5127 0.57 -0.0
5,704,796 - -1.1
5,704,796 - -0.0
5,704,796 - Pf1N1B4_5127 0.57 -3.4
5,704,796 - Pf1N1B4_5127 0.57 -0.0
5,704,799 + Pf1N1B4_5127 0.58 -3.2
5,704,799 + Pf1N1B4_5127 0.58 -0.0
5,704,800 - -0.0
5,704,800 - Pf1N1B4_5127 0.58 -2.5
5,704,800 - Pf1N1B4_5127 0.58 -0.0
5,705,055 + -0.0
5,705,055 + Pf1N1B4_5127 0.84 -3.1
5,705,055 + Pf1N1B4_5127 0.84 -0.0
5,705,055 + Pf1N1B4_5127 0.84 -1.6
5,705,055 + Pf1N1B4_5127 0.84 -0.0
5,705,055 + Pf1N1B4_5127 0.84 -3.3
5,705,055 + Pf1N1B4_5127 0.84 -4.4
5,705,056 - Pf1N1B4_5127 0.84 -0.0
5,705,056 - Pf1N1B4_5127 0.84 -0.0
5,705,234 + -0.0
5,705,248 + -0.0
5,705,249 - -3.0
5,705,249 - -0.0
5,705,432 - Pf1N1B4_5128 0.23 -0.7
5,705,432 - Pf1N1B4_5128 0.23 -0.0
5,705,581 + Pf1N1B4_5128 0.43 -0.0
5,705,581 + Pf1N1B4_5128 0.43 -1.7
5,705,581 + Pf1N1B4_5128 0.43 -0.0
5,705,582 - Pf1N1B4_5128 0.43 -1.7
5,705,582 - -1.2
5,705,586 - Pf1N1B4_5128 0.44 +1.6
5,705,723 - Pf1N1B4_5128 0.62 -2.0
5,705,757 - Pf1N1B4_5128 0.66 -0.0
5,705,882 - Pf1N1B4_5128 0.83 -0.0
5,705,894 + Pf1N1B4_5128 0.84 -0.0
5,705,894 + Pf1N1B4_5128 0.84 -0.0
5,705,895 - -1.0
5,705,896 + Pf1N1B4_5128 0.84 -0.0
5,705,896 + Pf1N1B4_5128 0.84 -0.4
5,705,896 + Pf1N1B4_5128 0.84 -0.0
5,705,896 + Pf1N1B4_5128 0.84 -0.0
5,705,896 + -0.0
5,705,897 - Pf1N1B4_5128 0.85 -0.0
5,705,897 - Pf1N1B4_5128 0.85 -1.5
5,705,897 - Pf1N1B4_5128 0.85 -2.1
5,705,897 - Pf1N1B4_5128 0.85 -1.5
5,705,897 - Pf1N1B4_5128 0.85 -0.0
5,705,897 - Pf1N1B4_5128 0.85 -0.0
5,705,954 + -1.5
5,706,051 + -1.3
5,706,052 - -0.0
5,706,158 + -0.0
5,706,158 + -2.7
5,706,158 + -0.0
5,706,158 + -0.6
5,706,159 - -1.5
5,706,159 - -0.0

Or see this region's nucleotide sequence