Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5061

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5060 and Pf1N1B4_5061 overlap by 4 nucleotidesPf1N1B4_5061 and Pf1N1B4_5062 are separated by 259 nucleotides Pf1N1B4_5060 - Ferrichrome-iron receptor, at 5,604,935 to 5,607,097 _5060 Pf1N1B4_5061 - Valyl-tRNA synthetase, at 5,607,094 to 5,607,402 _5061 Pf1N1B4_5062 - Homospermidine synthase (EC 2.5.1.44), at 5,607,662 to 5,609,086 _5062 Position (kb) 5607 5608Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 5606.180 kb on + strand, within Pf1N1B4_5060at 5606.338 kb on + strandat 5606.425 kb on + strand, within Pf1N1B4_5060at 5606.528 kb on + strand, within Pf1N1B4_5060at 5606.611 kb on + strandat 5606.753 kb on + strand, within Pf1N1B4_5060at 5606.753 kb on + strand, within Pf1N1B4_5060at 5606.754 kb on - strand, within Pf1N1B4_5060at 5606.909 kb on + strandat 5606.937 kb on - strandat 5607.026 kb on + strandat 5607.026 kb on + strandat 5607.026 kb on + strandat 5607.027 kb on - strandat 5607.027 kb on - strandat 5607.027 kb on - strandat 5607.134 kb on + strand, within Pf1N1B4_5061at 5607.135 kb on - strand, within Pf1N1B4_5061at 5607.147 kb on + strand, within Pf1N1B4_5061at 5607.179 kb on + strand, within Pf1N1B4_5061at 5607.179 kb on + strand, within Pf1N1B4_5061at 5607.179 kb on + strand, within Pf1N1B4_5061at 5607.180 kb on - strand, within Pf1N1B4_5061at 5607.180 kb on - strand, within Pf1N1B4_5061at 5607.876 kb on - strand, within Pf1N1B4_5062at 5608.024 kb on + strandat 5608.025 kb on - strand, within Pf1N1B4_5062at 5608.062 kb on - strand, within Pf1N1B4_5062at 5608.062 kb on - strand, within Pf1N1B4_5062at 5608.062 kb on - strand, within Pf1N1B4_5062

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
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5,606,180 + Pf1N1B4_5060 0.58 -0.0
5,606,338 + -0.0
5,606,425 + Pf1N1B4_5060 0.69 -0.0
5,606,528 + Pf1N1B4_5060 0.74 -1.6
5,606,611 + -0.0
5,606,753 + Pf1N1B4_5060 0.84 -2.9
5,606,753 + Pf1N1B4_5060 0.84 -1.1
5,606,754 - Pf1N1B4_5060 0.84 -0.0
5,606,909 + -0.0
5,606,937 - -2.7
5,607,026 + -0.0
5,607,026 + -0.0
5,607,026 + -1.9
5,607,027 - -0.0
5,607,027 - -0.0
5,607,027 - -0.0
5,607,134 + Pf1N1B4_5061 0.13 -0.0
5,607,135 - Pf1N1B4_5061 0.13 -0.0
5,607,147 + Pf1N1B4_5061 0.17 -2.3
5,607,179 + Pf1N1B4_5061 0.28 -0.0
5,607,179 + Pf1N1B4_5061 0.28 -0.0
5,607,179 + Pf1N1B4_5061 0.28 -4.2
5,607,180 - Pf1N1B4_5061 0.28 -0.0
5,607,180 - Pf1N1B4_5061 0.28 -3.1
5,607,876 - Pf1N1B4_5062 0.15 -0.0
5,608,024 + -2.7
5,608,025 - Pf1N1B4_5062 0.25 -1.6
5,608,062 - Pf1N1B4_5062 0.28 -1.4
5,608,062 - Pf1N1B4_5062 0.28 -0.0
5,608,062 - Pf1N1B4_5062 0.28 -0.3

Or see this region's nucleotide sequence