Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4937 and Pf1N1B4_4938 are separated by 190 nucleotides Pf1N1B4_4938 and Pf1N1B4_4939 are separated by 205 nucleotides Pf1N1B4_4939 and Pf1N1B4_4940 are separated by 55 nucleotides Pf1N1B4_4940 and Pf1N1B4_4941 are separated by 169 nucleotides
Pf1N1B4_4937 - Two-component hybrid sensor and regulator, at 5,473,465 to 5,475,384
_4937
Pf1N1B4_4938 - Regulator of cell morphogenesis and NO signaling, at 5,475,575 to 5,476,051
_4938
Pf1N1B4_4939 - sodium/hydrogen exchanger family protein, at 5,476,257 to 5,477,603
_4939
Pf1N1B4_4940 - probable membrane protein YPO2362, at 5,477,659 to 5,478,270
_4940
Pf1N1B4_4941 - competence/damage-inducible protein CinA, at 5,478,440 to 5,478,952
_4941
Position (kb) 5476
5477
5478 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 5475.399 kb on + strand at 5475.399 kb on + strand at 5475.550 kb on - strand at 5475.573 kb on - strand at 5475.841 kb on - strand, within Pf1N1B4_4938 at 5475.844 kb on - strand, within Pf1N1B4_4938 at 5475.844 kb on - strand, within Pf1N1B4_4938 at 5476.011 kb on - strand at 5476.069 kb on - strand at 5476.069 kb on - strand at 5476.307 kb on + strand at 5476.405 kb on + strand, within Pf1N1B4_4939 at 5476.417 kb on + strand, within Pf1N1B4_4939 at 5476.417 kb on + strand, within Pf1N1B4_4939 at 5476.418 kb on - strand at 5476.873 kb on + strand, within Pf1N1B4_4939 at 5476.873 kb on + strand, within Pf1N1B4_4939 at 5476.874 kb on - strand, within Pf1N1B4_4939 at 5477.162 kb on - strand, within Pf1N1B4_4939 at 5477.537 kb on - strand at 5477.590 kb on - strand at 5477.678 kb on - strand at 5477.681 kb on + strand at 5477.697 kb on - strand at 5477.731 kb on - strand, within Pf1N1B4_4940 at 5477.844 kb on - strand, within Pf1N1B4_4940 at 5478.183 kb on - strand, within Pf1N1B4_4940 at 5478.216 kb on + strand at 5478.284 kb on + strand at 5478.316 kb on + strand at 5478.316 kb on - strand at 5478.317 kb on - strand at 5478.587 kb on + strand, within Pf1N1B4_4941
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 5,475,399 + -2.2 5,475,399 + -0.0 5,475,550 - -2.9 5,475,573 - -2.7 5,475,841 - Pf1N1B4_4938 0.56 -0.0 5,475,844 - Pf1N1B4_4938 0.56 -2.1 5,475,844 - Pf1N1B4_4938 0.56 -2.9 5,476,011 - -0.0 5,476,069 - -0.0 5,476,069 - -0.0 5,476,307 + -0.0 5,476,405 + Pf1N1B4_4939 0.11 -0.0 5,476,417 + Pf1N1B4_4939 0.12 -0.0 5,476,417 + Pf1N1B4_4939 0.12 -1.9 5,476,418 - -0.0 5,476,873 + Pf1N1B4_4939 0.46 -0.0 5,476,873 + Pf1N1B4_4939 0.46 -5.1 5,476,874 - Pf1N1B4_4939 0.46 -2.7 5,477,162 - Pf1N1B4_4939 0.67 -0.0 5,477,537 - -0.0 5,477,590 - -1.8 5,477,678 - -1.2 5,477,681 + +0.3 5,477,697 - -1.0 5,477,731 - Pf1N1B4_4940 0.12 -0.0 5,477,844 - Pf1N1B4_4940 0.30 -0.0 5,478,183 - Pf1N1B4_4940 0.86 -2.0 5,478,216 + -3.3 5,478,284 + -0.0 5,478,316 + -0.0 5,478,316 - -0.0 5,478,317 - -0.0 5,478,587 + Pf1N1B4_4941 0.29 -2.7
Or see this region's nucleotide sequence