Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4919 and Pf1N1B4_4920 are separated by 240 nucleotides Pf1N1B4_4920 and Pf1N1B4_4921 are separated by 466 nucleotides
Pf1N1B4_4919 - Histone acetyltransferase HPA2 and related acetyltransferases, at 5,450,476 to 5,451,333
_4919
Pf1N1B4_4920 - Aldehyde dehydrogenase (EC 1.2.1.3), at 5,451,574 to 5,453,046
_4920
Pf1N1B4_4921 - Haloacid dehalogenase, type II, at 5,453,513 to 5,454,181
_4921
Position (kb) 5451
5452
5453
5454 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5450.993 kb on + strand at 5451.095 kb on + strand, within Pf1N1B4_4919 at 5451.308 kb on + strand at 5451.309 kb on - strand at 5451.460 kb on + strand at 5451.461 kb on - strand at 5451.473 kb on + strand at 5451.473 kb on + strand at 5451.474 kb on - strand at 5451.474 kb on - strand at 5451.474 kb on - strand at 5451.474 kb on - strand at 5451.474 kb on - strand at 5451.474 kb on - strand at 5451.474 kb on - strand at 5451.476 kb on + strand at 5451.477 kb on - strand at 5451.480 kb on + strand at 5451.481 kb on - strand at 5451.481 kb on - strand at 5451.481 kb on - strand at 5451.524 kb on + strand at 5451.579 kb on + strand at 5451.622 kb on - strand at 5451.688 kb on + strand at 5451.757 kb on - strand, within Pf1N1B4_4920 at 5451.893 kb on + strand, within Pf1N1B4_4920 at 5451.913 kb on + strand, within Pf1N1B4_4920 at 5452.225 kb on + strand, within Pf1N1B4_4920 at 5452.519 kb on + strand, within Pf1N1B4_4920 at 5452.687 kb on + strand, within Pf1N1B4_4920 at 5452.688 kb on - strand, within Pf1N1B4_4920 at 5452.934 kb on - strand at 5453.288 kb on - strand at 5453.419 kb on + strand at 5453.420 kb on - strand at 5453.772 kb on - strand, within Pf1N1B4_4921 at 5453.774 kb on + strand, within Pf1N1B4_4921 at 5453.775 kb on - strand, within Pf1N1B4_4921 at 5454.041 kb on + strand, within Pf1N1B4_4921
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 5,450,993 + -1.4 5,451,095 + Pf1N1B4_4919 0.72 -0.0 5,451,308 + -0.7 5,451,309 - -2.5 5,451,460 + -1.0 5,451,461 - -0.0 5,451,473 + -1.9 5,451,473 + -0.0 5,451,474 - -1.6 5,451,474 - -0.0 5,451,474 - -1.1 5,451,474 - -0.0 5,451,474 - -0.0 5,451,474 - -0.0 5,451,474 - -0.8 5,451,476 + -1.1 5,451,477 - -1.6 5,451,480 + -2.2 5,451,481 - -1.8 5,451,481 - -1.7 5,451,481 - -0.0 5,451,524 + -0.5 5,451,579 + -0.0 5,451,622 - -0.0 5,451,688 + -0.0 5,451,757 - Pf1N1B4_4920 0.12 -0.0 5,451,893 + Pf1N1B4_4920 0.22 -0.0 5,451,913 + Pf1N1B4_4920 0.23 -2.4 5,452,225 + Pf1N1B4_4920 0.44 -3.1 5,452,519 + Pf1N1B4_4920 0.64 -2.5 5,452,687 + Pf1N1B4_4920 0.76 -0.0 5,452,688 - Pf1N1B4_4920 0.76 -2.7 5,452,934 - -0.0 5,453,288 - -2.1 5,453,419 + -0.0 5,453,420 - -0.0 5,453,772 - Pf1N1B4_4921 0.39 -1.0 5,453,774 + Pf1N1B4_4921 0.39 -0.0 5,453,775 - Pf1N1B4_4921 0.39 -0.0 5,454,041 + Pf1N1B4_4921 0.79 -2.1
Or see this region's nucleotide sequence