Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4865 and Pf1N1B4_4866 are separated by 307 nucleotides Pf1N1B4_4866 and Pf1N1B4_4867 are separated by 40 nucleotides
Pf1N1B4_4865 - Multidrug efflux membrane fusion protein MexE, at 5,382,967 to 5,384,220
_4865
Pf1N1B4_4866 - hypothetical protein, at 5,384,528 to 5,384,653
_4866
Pf1N1B4_4867 - FIG00464907: hypothetical protein, at 5,384,694 to 5,385,713
_4867
Position (kb)
5384
5385 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 5383.554 kb on - strand, within Pf1N1B4_4865 at 5383.557 kb on + strand, within Pf1N1B4_4865 at 5383.558 kb on - strand, within Pf1N1B4_4865 at 5383.558 kb on - strand, within Pf1N1B4_4865 at 5383.689 kb on + strand, within Pf1N1B4_4865 at 5384.239 kb on - strand at 5384.272 kb on + strand at 5384.272 kb on + strand at 5384.273 kb on - strand at 5384.273 kb on - strand at 5384.318 kb on + strand at 5384.320 kb on + strand at 5384.397 kb on + strand at 5384.529 kb on + strand at 5384.530 kb on - strand at 5384.530 kb on - strand at 5384.561 kb on + strand, within Pf1N1B4_4866 at 5384.561 kb on + strand, within Pf1N1B4_4866 at 5384.599 kb on + strand, within Pf1N1B4_4866 at 5384.615 kb on - strand, within Pf1N1B4_4866 at 5384.633 kb on + strand, within Pf1N1B4_4866 at 5384.633 kb on + strand, within Pf1N1B4_4866 at 5385.195 kb on + strand, within Pf1N1B4_4867 at 5385.257 kb on + strand, within Pf1N1B4_4867 at 5385.257 kb on + strand, within Pf1N1B4_4867 at 5385.257 kb on + strand at 5385.257 kb on + strand, within Pf1N1B4_4867 at 5385.258 kb on - strand, within Pf1N1B4_4867 at 5385.258 kb on - strand, within Pf1N1B4_4867 at 5385.381 kb on + strand, within Pf1N1B4_4867 at 5385.381 kb on + strand, within Pf1N1B4_4867 at 5385.382 kb on - strand, within Pf1N1B4_4867 at 5385.485 kb on - strand, within Pf1N1B4_4867 at 5385.498 kb on + strand, within Pf1N1B4_4867
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 5,383,554 - Pf1N1B4_4865 0.47 -0.1 5,383,557 + Pf1N1B4_4865 0.47 -2.2 5,383,558 - Pf1N1B4_4865 0.47 -3.1 5,383,558 - Pf1N1B4_4865 0.47 -1.5 5,383,689 + Pf1N1B4_4865 0.58 -1.9 5,384,239 - -1.6 5,384,272 + -2.0 5,384,272 + -0.1 5,384,273 - -1.7 5,384,273 - -1.8 5,384,318 + -0.8 5,384,320 + -0.1 5,384,397 + -2.9 5,384,529 + -0.1 5,384,530 - -1.9 5,384,530 - -0.1 5,384,561 + Pf1N1B4_4866 0.26 -3.8 5,384,561 + Pf1N1B4_4866 0.26 -1.7 5,384,599 + Pf1N1B4_4866 0.56 -2.6 5,384,615 - Pf1N1B4_4866 0.69 -0.1 5,384,633 + Pf1N1B4_4866 0.83 -0.1 5,384,633 + Pf1N1B4_4866 0.83 -0.1 5,385,195 + Pf1N1B4_4867 0.49 -0.1 5,385,257 + Pf1N1B4_4867 0.55 -0.1 5,385,257 + Pf1N1B4_4867 0.55 -0.1 5,385,257 + -0.1 5,385,257 + Pf1N1B4_4867 0.55 -0.1 5,385,258 - Pf1N1B4_4867 0.55 -3.1 5,385,258 - Pf1N1B4_4867 0.55 -0.1 5,385,381 + Pf1N1B4_4867 0.67 -1.1 5,385,381 + Pf1N1B4_4867 0.67 -3.1 5,385,382 - Pf1N1B4_4867 0.67 -0.1 5,385,485 - Pf1N1B4_4867 0.78 -0.1 5,385,498 + Pf1N1B4_4867 0.79 -0.1
Or see this region's nucleotide sequence