Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4846 and Pf1N1B4_4847 are separated by 14 nucleotides Pf1N1B4_4847 and Pf1N1B4_4848 are separated by 26 nucleotides Pf1N1B4_4848 and Pf1N1B4_4849 are separated by 10 nucleotides
Pf1N1B4_4846 - Multiple polyol-specific dehydrogenase (EC 1.1.1.-), at 5,354,376 to 5,355,851
_4846
Pf1N1B4_4847 - Various polyols ABC transporter, ATP-binding component, at 5,355,866 to 5,356,969
_4847
Pf1N1B4_4848 - Various polyols ABC transporter, permease component 2, at 5,356,996 to 5,357,826
_4848
Pf1N1B4_4849 - Various polyols ABC transporter, permease component 1, at 5,357,837 to 5,358,736
_4849
Position (kb) 5355
5356
5357 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5354.929 kb on + strand at 5354.930 kb on - strand, within Pf1N1B4_4846 at 5355.610 kb on + strand, within Pf1N1B4_4846 at 5355.610 kb on + strand, within Pf1N1B4_4846 at 5355.718 kb on + strand at 5355.719 kb on - strand at 5355.794 kb on - strand at 5356.404 kb on + strand, within Pf1N1B4_4847 at 5356.404 kb on + strand, within Pf1N1B4_4847 at 5356.404 kb on + strand, within Pf1N1B4_4847 at 5356.404 kb on + strand, within Pf1N1B4_4847 at 5356.405 kb on - strand, within Pf1N1B4_4847 at 5356.720 kb on - strand, within Pf1N1B4_4847 at 5356.720 kb on - strand, within Pf1N1B4_4847 at 5356.994 kb on - strand at 5357.342 kb on + strand, within Pf1N1B4_4848 at 5357.343 kb on - strand, within Pf1N1B4_4848 at 5357.343 kb on - strand at 5357.348 kb on + strand, within Pf1N1B4_4848 at 5357.349 kb on - strand, within Pf1N1B4_4848 at 5357.387 kb on + strand, within Pf1N1B4_4848 at 5357.445 kb on - strand, within Pf1N1B4_4848 at 5357.528 kb on + strand, within Pf1N1B4_4848 at 5357.529 kb on - strand, within Pf1N1B4_4848 at 5357.625 kb on - strand at 5357.651 kb on + strand, within Pf1N1B4_4848 at 5357.651 kb on + strand, within Pf1N1B4_4848 at 5357.651 kb on + strand at 5357.652 kb on - strand, within Pf1N1B4_4848
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 5,354,929 + -0.6 5,354,930 - Pf1N1B4_4846 0.38 -0.0 5,355,610 + Pf1N1B4_4846 0.84 -1.1 5,355,610 + Pf1N1B4_4846 0.84 -0.0 5,355,718 + -0.0 5,355,719 - -1.9 5,355,794 - -4.0 5,356,404 + Pf1N1B4_4847 0.49 -2.1 5,356,404 + Pf1N1B4_4847 0.49 -0.0 5,356,404 + Pf1N1B4_4847 0.49 -2.9 5,356,404 + Pf1N1B4_4847 0.49 -0.0 5,356,405 - Pf1N1B4_4847 0.49 -0.0 5,356,720 - Pf1N1B4_4847 0.77 -0.0 5,356,720 - Pf1N1B4_4847 0.77 -0.0 5,356,994 - -2.7 5,357,342 + Pf1N1B4_4848 0.42 -0.0 5,357,343 - Pf1N1B4_4848 0.42 -1.6 5,357,343 - -1.8 5,357,348 + Pf1N1B4_4848 0.42 -0.0 5,357,349 - Pf1N1B4_4848 0.42 -1.5 5,357,387 + Pf1N1B4_4848 0.47 -1.8 5,357,445 - Pf1N1B4_4848 0.54 -1.8 5,357,528 + Pf1N1B4_4848 0.64 -0.0 5,357,529 - Pf1N1B4_4848 0.64 -2.9 5,357,625 - -3.6 5,357,651 + Pf1N1B4_4848 0.79 -2.0 5,357,651 + Pf1N1B4_4848 0.79 -0.0 5,357,651 + -0.0 5,357,652 - Pf1N1B4_4848 0.79 -0.0
Or see this region's nucleotide sequence