Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4812

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4810 and Pf1N1B4_4811 overlap by 4 nucleotidesPf1N1B4_4811 and Pf1N1B4_4812 are separated by 575 nucleotidesPf1N1B4_4812 and Pf1N1B4_4813 are separated by 9 nucleotidesPf1N1B4_4813 and Pf1N1B4_4814 overlap by 7 nucleotides Pf1N1B4_4810 - Polymyxin resistance protein PmrM, at 5,313,391 to 5,313,804 _4810 Pf1N1B4_4811 - Polymyxin resistance protein PmrL, sucrose-6 phosphate hydrolase, at 5,313,801 to 5,314,145 _4811 Pf1N1B4_4812 - Site-specific recombinase, phage integrase family, at 5,314,721 to 5,315,692 _4812 Pf1N1B4_4813 - Iron(III) dicitrate transport ATP-binding protein FecE (TC 3.A.1.14.1), at 5,315,702 to 5,316,457 _4813 Pf1N1B4_4814 - Iron(III) dicitrate transport system permease protein FecD (TC 3.A.1.14.1), at 5,316,451 to 5,317,512 _4814 Position (kb) 5314 5315 5316Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 5314.129 kb on - strandat 5314.356 kb on - strandat 5314.379 kb on + strandat 5314.379 kb on + strandat 5314.380 kb on - strandat 5314.380 kb on - strandat 5314.663 kb on - strandat 5314.691 kb on - strandat 5314.693 kb on - strandat 5314.700 kb on - strandat 5314.765 kb on - strandat 5314.812 kb on - strandat 5314.812 kb on - strandat 5314.853 kb on + strand, within Pf1N1B4_4812at 5314.858 kb on - strand, within Pf1N1B4_4812at 5314.858 kb on - strand, within Pf1N1B4_4812at 5314.976 kb on + strand, within Pf1N1B4_4812at 5315.001 kb on - strand, within Pf1N1B4_4812at 5315.391 kb on + strand, within Pf1N1B4_4812at 5315.483 kb on + strand, within Pf1N1B4_4812at 5315.486 kb on + strand, within Pf1N1B4_4812at 5315.486 kb on + strand, within Pf1N1B4_4812at 5315.486 kb on + strandat 5315.487 kb on - strandat 5315.636 kb on + strandat 5315.636 kb on + strandat 5315.637 kb on - strandat 5315.637 kb on - strandat 5315.637 kb on - strandat 5315.773 kb on - strandat 5315.784 kb on - strand, within Pf1N1B4_4813at 5316.237 kb on + strand, within Pf1N1B4_4813at 5316.536 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
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5,314,129 - -3.9
5,314,356 - -0.0
5,314,379 + -0.0
5,314,379 + -1.5
5,314,380 - -0.0
5,314,380 - -0.0
5,314,663 - -0.0
5,314,691 - -0.0
5,314,693 - -1.6
5,314,700 - -2.0
5,314,765 - -0.0
5,314,812 - -0.0
5,314,812 - -0.0
5,314,853 + Pf1N1B4_4812 0.14 -1.3
5,314,858 - Pf1N1B4_4812 0.14 -2.0
5,314,858 - Pf1N1B4_4812 0.14 -3.2
5,314,976 + Pf1N1B4_4812 0.26 -0.0
5,315,001 - Pf1N1B4_4812 0.29 -2.7
5,315,391 + Pf1N1B4_4812 0.69 -2.7
5,315,483 + Pf1N1B4_4812 0.78 -0.0
5,315,486 + Pf1N1B4_4812 0.79 -0.0
5,315,486 + Pf1N1B4_4812 0.79 -0.0
5,315,486 + -0.0
5,315,487 - -0.1
5,315,636 + -3.7
5,315,636 + -1.2
5,315,637 - -2.7
5,315,637 - -5.0
5,315,637 - -1.6
5,315,773 - -0.8
5,315,784 - Pf1N1B4_4813 0.11 -2.6
5,316,237 + Pf1N1B4_4813 0.71 -1.9
5,316,536 - -0.0

Or see this region's nucleotide sequence