Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4810 and Pf1N1B4_4811 overlap by 4 nucleotides Pf1N1B4_4811 and Pf1N1B4_4812 are separated by 575 nucleotides Pf1N1B4_4812 and Pf1N1B4_4813 are separated by 9 nucleotides Pf1N1B4_4813 and Pf1N1B4_4814 overlap by 7 nucleotides
Pf1N1B4_4810 - Polymyxin resistance protein PmrM, at 5,313,391 to 5,313,804
_4810
Pf1N1B4_4811 - Polymyxin resistance protein PmrL, sucrose-6 phosphate hydrolase, at 5,313,801 to 5,314,145
_4811
Pf1N1B4_4812 - Site-specific recombinase, phage integrase family, at 5,314,721 to 5,315,692
_4812
Pf1N1B4_4813 - Iron(III) dicitrate transport ATP-binding protein FecE (TC 3.A.1.14.1), at 5,315,702 to 5,316,457
_4813
Pf1N1B4_4814 - Iron(III) dicitrate transport system permease protein FecD (TC 3.A.1.14.1), at 5,316,451 to 5,317,512
_4814
Position (kb)
5314
5315
5316 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 5314.129 kb on - strand at 5314.356 kb on - strand at 5314.379 kb on + strand at 5314.379 kb on + strand at 5314.380 kb on - strand at 5314.380 kb on - strand at 5314.663 kb on - strand at 5314.691 kb on - strand at 5314.693 kb on - strand at 5314.700 kb on - strand at 5314.765 kb on - strand at 5314.812 kb on - strand at 5314.812 kb on - strand at 5314.853 kb on + strand, within Pf1N1B4_4812 at 5314.858 kb on - strand, within Pf1N1B4_4812 at 5314.858 kb on - strand, within Pf1N1B4_4812 at 5314.976 kb on + strand, within Pf1N1B4_4812 at 5315.001 kb on - strand, within Pf1N1B4_4812 at 5315.391 kb on + strand, within Pf1N1B4_4812 at 5315.483 kb on + strand, within Pf1N1B4_4812 at 5315.486 kb on + strand, within Pf1N1B4_4812 at 5315.486 kb on + strand, within Pf1N1B4_4812 at 5315.486 kb on + strand at 5315.487 kb on - strand at 5315.636 kb on + strand at 5315.636 kb on + strand at 5315.637 kb on - strand at 5315.637 kb on - strand at 5315.637 kb on - strand at 5315.773 kb on - strand at 5315.784 kb on - strand, within Pf1N1B4_4813 at 5316.237 kb on + strand, within Pf1N1B4_4813 at 5316.536 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 5,314,129 - -3.9 5,314,356 - -0.0 5,314,379 + -0.0 5,314,379 + -1.5 5,314,380 - -0.0 5,314,380 - -0.0 5,314,663 - -0.0 5,314,691 - -0.0 5,314,693 - -1.6 5,314,700 - -2.0 5,314,765 - -0.0 5,314,812 - -0.0 5,314,812 - -0.0 5,314,853 + Pf1N1B4_4812 0.14 -1.3 5,314,858 - Pf1N1B4_4812 0.14 -2.0 5,314,858 - Pf1N1B4_4812 0.14 -3.2 5,314,976 + Pf1N1B4_4812 0.26 -0.0 5,315,001 - Pf1N1B4_4812 0.29 -2.7 5,315,391 + Pf1N1B4_4812 0.69 -2.7 5,315,483 + Pf1N1B4_4812 0.78 -0.0 5,315,486 + Pf1N1B4_4812 0.79 -0.0 5,315,486 + Pf1N1B4_4812 0.79 -0.0 5,315,486 + -0.0 5,315,487 - -0.1 5,315,636 + -3.7 5,315,636 + -1.2 5,315,637 - -2.7 5,315,637 - -5.0 5,315,637 - -1.6 5,315,773 - -0.8 5,315,784 - Pf1N1B4_4813 0.11 -2.6 5,316,237 + Pf1N1B4_4813 0.71 -1.9 5,316,536 - -0.0
Or see this region's nucleotide sequence