Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4735 and Pf1N1B4_4736 are separated by 51 nucleotides Pf1N1B4_4736 and Pf1N1B4_4737 are separated by 1 nucleotides Pf1N1B4_4737 and Pf1N1B4_4738 are separated by 698 nucleotides
Pf1N1B4_4735 - Fap amyloid fibril major component, at 5,232,665 to 5,233,954
_4735
Pf1N1B4_4736 - Fap amyloid fibril minor component, at 5,234,006 to 5,234,578
_4736
Pf1N1B4_4737 - hypothetical protein, at 5,234,580 to 5,234,801
_4737
Pf1N1B4_4738 - Ribosylnicotinamide kinase (EC 2.7.1.22), at 5,235,500 to 5,236,024
_4738
Position (kb) 5234
5235 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5233.035 kb on - strand, within Pf1N1B4_4735 at 5233.035 kb on - strand, within Pf1N1B4_4735 at 5233.349 kb on - strand, within Pf1N1B4_4735 at 5233.815 kb on + strand, within Pf1N1B4_4735 at 5233.847 kb on - strand at 5233.889 kb on + strand at 5233.890 kb on - strand at 5233.968 kb on - strand at 5233.971 kb on + strand at 5233.971 kb on + strand at 5233.971 kb on + strand at 5233.982 kb on - strand at 5233.994 kb on + strand at 5234.021 kb on + strand at 5234.218 kb on + strand, within Pf1N1B4_4736 at 5234.219 kb on - strand, within Pf1N1B4_4736 at 5234.224 kb on + strand, within Pf1N1B4_4736 at 5234.224 kb on + strand, within Pf1N1B4_4736 at 5234.225 kb on - strand, within Pf1N1B4_4736 at 5234.228 kb on + strand, within Pf1N1B4_4736 at 5234.735 kb on + strand at 5235.233 kb on + strand at 5235.233 kb on + strand at 5235.233 kb on + strand at 5235.234 kb on - strand at 5235.244 kb on + strand at 5235.353 kb on + strand at 5235.353 kb on + strand at 5235.439 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 5,233,035 - Pf1N1B4_4735 0.29 -0.0 5,233,035 - Pf1N1B4_4735 0.29 -1.1 5,233,349 - Pf1N1B4_4735 0.53 -0.0 5,233,815 + Pf1N1B4_4735 0.89 -0.0 5,233,847 - -2.3 5,233,889 + -0.0 5,233,890 - -2.0 5,233,968 - -2.0 5,233,971 + -0.0 5,233,971 + -0.0 5,233,971 + -1.7 5,233,982 - -0.0 5,233,994 + -0.0 5,234,021 + -0.0 5,234,218 + Pf1N1B4_4736 0.37 -2.9 5,234,219 - Pf1N1B4_4736 0.37 -1.3 5,234,224 + Pf1N1B4_4736 0.38 -1.9 5,234,224 + Pf1N1B4_4736 0.38 -0.0 5,234,225 - Pf1N1B4_4736 0.38 -0.0 5,234,228 + Pf1N1B4_4736 0.39 -2.9 5,234,735 + -1.6 5,235,233 + -0.0 5,235,233 + -0.0 5,235,233 + -0.0 5,235,234 - -0.0 5,235,244 + -3.1 5,235,353 + -0.0 5,235,353 + -0.0 5,235,439 + -0.0
Or see this region's nucleotide sequence