Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4726 and Pf1N1B4_4727 overlap by 4 nucleotides Pf1N1B4_4727 and Pf1N1B4_4728 are separated by 115 nucleotides
Pf1N1B4_4726 - Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49), at 5,222,158 to 5,223,681
_4726
Pf1N1B4_4727 - HMP-PP hydrolase (pyridoxal phosphatase) Cof, detected in genetic screen for thiamin metabolic genes (PMID:15292217), at 5,223,678 to 5,224,499
_4727
Pf1N1B4_4728 - Na+/H+ antiporter NhaB, at 5,224,615 to 5,226,117
_4728
Position (kb) 5223
5224
5225 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5222.746 kb on + strand, within Pf1N1B4_4726 at 5222.747 kb on - strand, within Pf1N1B4_4726 at 5222.943 kb on - strand, within Pf1N1B4_4726 at 5223.160 kb on + strand, within Pf1N1B4_4726 at 5223.160 kb on + strand, within Pf1N1B4_4726 at 5223.161 kb on - strand, within Pf1N1B4_4726 at 5223.215 kb on - strand, within Pf1N1B4_4726 at 5223.215 kb on - strand at 5223.215 kb on - strand, within Pf1N1B4_4726 at 5223.328 kb on + strand, within Pf1N1B4_4726 at 5223.329 kb on - strand, within Pf1N1B4_4726 at 5223.484 kb on + strand, within Pf1N1B4_4726 at 5223.484 kb on + strand at 5223.485 kb on - strand, within Pf1N1B4_4726 at 5223.485 kb on - strand, within Pf1N1B4_4726 at 5223.485 kb on - strand, within Pf1N1B4_4726 at 5223.485 kb on - strand, within Pf1N1B4_4726 at 5223.485 kb on - strand, within Pf1N1B4_4726 at 5223.910 kb on + strand, within Pf1N1B4_4727 at 5223.942 kb on + strand, within Pf1N1B4_4727 at 5223.942 kb on + strand, within Pf1N1B4_4727 at 5223.942 kb on + strand, within Pf1N1B4_4727 at 5223.942 kb on + strand, within Pf1N1B4_4727 at 5223.943 kb on - strand, within Pf1N1B4_4727 at 5224.119 kb on + strand at 5224.242 kb on + strand, within Pf1N1B4_4727 at 5224.242 kb on + strand, within Pf1N1B4_4727 at 5224.482 kb on + strand at 5224.482 kb on + strand at 5224.482 kb on + strand at 5224.482 kb on + strand at 5224.483 kb on - strand at 5224.483 kb on - strand at 5224.483 kb on - strand at 5224.483 kb on - strand at 5224.483 kb on - strand at 5224.483 kb on - strand at 5224.617 kb on - strand at 5224.617 kb on - strand at 5224.619 kb on - strand at 5224.626 kb on - strand at 5224.638 kb on - strand at 5224.665 kb on + strand at 5224.666 kb on - strand at 5224.666 kb on - strand at 5224.668 kb on - strand at 5224.875 kb on - strand, within Pf1N1B4_4728 at 5224.875 kb on - strand, within Pf1N1B4_4728 at 5224.875 kb on - strand, within Pf1N1B4_4728 at 5224.875 kb on - strand, within Pf1N1B4_4728 at 5224.930 kb on - strand, within Pf1N1B4_4728
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 5,222,746 + Pf1N1B4_4726 0.39 -0.0 5,222,747 - Pf1N1B4_4726 0.39 -1.8 5,222,943 - Pf1N1B4_4726 0.52 -1.2 5,223,160 + Pf1N1B4_4726 0.66 -4.0 5,223,160 + Pf1N1B4_4726 0.66 -0.0 5,223,161 - Pf1N1B4_4726 0.66 -0.0 5,223,215 - Pf1N1B4_4726 0.69 -0.0 5,223,215 - -0.0 5,223,215 - Pf1N1B4_4726 0.69 -0.4 5,223,328 + Pf1N1B4_4726 0.77 -0.0 5,223,329 - Pf1N1B4_4726 0.77 -2.7 5,223,484 + Pf1N1B4_4726 0.87 -1.7 5,223,484 + -0.0 5,223,485 - Pf1N1B4_4726 0.87 -0.0 5,223,485 - Pf1N1B4_4726 0.87 -0.0 5,223,485 - Pf1N1B4_4726 0.87 -0.0 5,223,485 - Pf1N1B4_4726 0.87 -0.0 5,223,485 - Pf1N1B4_4726 0.87 -1.9 5,223,910 + Pf1N1B4_4727 0.28 -0.0 5,223,942 + Pf1N1B4_4727 0.32 -0.0 5,223,942 + Pf1N1B4_4727 0.32 -3.2 5,223,942 + Pf1N1B4_4727 0.32 -0.0 5,223,942 + Pf1N1B4_4727 0.32 -0.0 5,223,943 - Pf1N1B4_4727 0.32 -0.0 5,224,119 + -1.5 5,224,242 + Pf1N1B4_4727 0.69 -0.0 5,224,242 + Pf1N1B4_4727 0.69 -0.6 5,224,482 + -0.0 5,224,482 + -1.3 5,224,482 + -0.0 5,224,482 + -1.7 5,224,483 - -0.0 5,224,483 - -2.3 5,224,483 - -0.0 5,224,483 - -0.0 5,224,483 - -1.1 5,224,483 - -0.0 5,224,617 - -1.3 5,224,617 - -1.6 5,224,619 - -2.4 5,224,626 - -1.0 5,224,638 - -0.0 5,224,665 + -0.5 5,224,666 - -0.0 5,224,666 - -0.0 5,224,668 - -0.0 5,224,875 - Pf1N1B4_4728 0.17 -0.0 5,224,875 - Pf1N1B4_4728 0.17 -2.3 5,224,875 - Pf1N1B4_4728 0.17 -2.3 5,224,875 - Pf1N1B4_4728 0.17 -0.0 5,224,930 - Pf1N1B4_4728 0.21 -1.8
Or see this region's nucleotide sequence