Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4669 and Pf1N1B4_4670 are separated by 258 nucleotides Pf1N1B4_4670 and Pf1N1B4_4671 are separated by 285 nucleotides
Pf1N1B4_4669 - Heavy metal RND efflux outer membrane protein, CzcC family, at 5,148,679 to 5,149,602
_4669
Pf1N1B4_4670 - Sensory box/GGDEF family protein, at 5,149,861 to 5,151,942
_4670
Pf1N1B4_4671 - Short-chain dehydrogenase/reductase SDR, at 5,152,228 to 5,152,974
_4671
Position (kb) 5149
5150
5151
5152 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5148.929 kb on + strand, within Pf1N1B4_4669 at 5149.105 kb on + strand, within Pf1N1B4_4669 at 5149.143 kb on + strand, within Pf1N1B4_4669 at 5149.287 kb on + strand at 5149.334 kb on - strand, within Pf1N1B4_4669 at 5149.334 kb on - strand, within Pf1N1B4_4669 at 5149.876 kb on + strand at 5149.876 kb on + strand at 5149.876 kb on + strand at 5149.884 kb on + strand at 5149.917 kb on - strand at 5149.927 kb on + strand at 5149.927 kb on + strand at 5149.927 kb on + strand at 5149.928 kb on - strand at 5149.929 kb on + strand at 5149.930 kb on - strand at 5149.930 kb on - strand at 5149.930 kb on - strand at 5150.227 kb on + strand, within Pf1N1B4_4670 at 5150.227 kb on + strand at 5150.227 kb on + strand at 5150.228 kb on - strand, within Pf1N1B4_4670 at 5150.228 kb on - strand, within Pf1N1B4_4670 at 5150.429 kb on - strand, within Pf1N1B4_4670 at 5150.429 kb on - strand, within Pf1N1B4_4670 at 5150.688 kb on - strand, within Pf1N1B4_4670 at 5151.088 kb on + strand, within Pf1N1B4_4670 at 5151.089 kb on - strand, within Pf1N1B4_4670 at 5151.217 kb on + strand, within Pf1N1B4_4670 at 5151.221 kb on - strand, within Pf1N1B4_4670 at 5151.221 kb on - strand, within Pf1N1B4_4670 at 5151.406 kb on + strand, within Pf1N1B4_4670 at 5151.407 kb on - strand at 5151.407 kb on - strand, within Pf1N1B4_4670 at 5151.646 kb on + strand, within Pf1N1B4_4670 at 5151.647 kb on - strand, within Pf1N1B4_4670 at 5151.865 kb on + strand at 5151.866 kb on - strand at 5152.113 kb on + strand at 5152.114 kb on - strand at 5152.114 kb on - strand at 5152.324 kb on + strand at 5152.687 kb on + strand, within Pf1N1B4_4671 at 5152.687 kb on + strand, within Pf1N1B4_4671 at 5152.688 kb on - strand, within Pf1N1B4_4671 at 5152.688 kb on - strand, within Pf1N1B4_4671 at 5152.688 kb on - strand, within Pf1N1B4_4671 at 5152.696 kb on + strand, within Pf1N1B4_4671 at 5152.799 kb on - strand, within Pf1N1B4_4671 at 5152.799 kb on - strand, within Pf1N1B4_4671
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 5,148,929 + Pf1N1B4_4669 0.27 -0.0 5,149,105 + Pf1N1B4_4669 0.46 -0.0 5,149,143 + Pf1N1B4_4669 0.50 -2.4 5,149,287 + -1.7 5,149,334 - Pf1N1B4_4669 0.71 -3.7 5,149,334 - Pf1N1B4_4669 0.71 -0.0 5,149,876 + -0.0 5,149,876 + -1.9 5,149,876 + -0.0 5,149,884 + -1.9 5,149,917 - -0.0 5,149,927 + -0.0 5,149,927 + -1.9 5,149,927 + -1.3 5,149,928 - -0.0 5,149,929 + -0.0 5,149,930 - -1.1 5,149,930 - -2.2 5,149,930 - -0.0 5,150,227 + Pf1N1B4_4670 0.18 -2.0 5,150,227 + -0.0 5,150,227 + -2.5 5,150,228 - Pf1N1B4_4670 0.18 -0.0 5,150,228 - Pf1N1B4_4670 0.18 -2.2 5,150,429 - Pf1N1B4_4670 0.27 -0.0 5,150,429 - Pf1N1B4_4670 0.27 -4.3 5,150,688 - Pf1N1B4_4670 0.40 -0.0 5,151,088 + Pf1N1B4_4670 0.59 -0.0 5,151,089 - Pf1N1B4_4670 0.59 -2.9 5,151,217 + Pf1N1B4_4670 0.65 -2.8 5,151,221 - Pf1N1B4_4670 0.65 -2.0 5,151,221 - Pf1N1B4_4670 0.65 -0.0 5,151,406 + Pf1N1B4_4670 0.74 -0.0 5,151,407 - -3.2 5,151,407 - Pf1N1B4_4670 0.74 -0.0 5,151,646 + Pf1N1B4_4670 0.86 -0.0 5,151,647 - Pf1N1B4_4670 0.86 -0.0 5,151,865 + -0.0 5,151,866 - +0.6 5,152,113 + -0.0 5,152,114 - -0.7 5,152,114 - -2.1 5,152,324 + -3.6 5,152,687 + Pf1N1B4_4671 0.61 -0.0 5,152,687 + Pf1N1B4_4671 0.61 -0.0 5,152,688 - Pf1N1B4_4671 0.62 -0.0 5,152,688 - Pf1N1B4_4671 0.62 -2.4 5,152,688 - Pf1N1B4_4671 0.62 -0.0 5,152,696 + Pf1N1B4_4671 0.63 -0.0 5,152,799 - Pf1N1B4_4671 0.76 -1.6 5,152,799 - Pf1N1B4_4671 0.76 -0.0
Or see this region's nucleotide sequence