Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4550 and Pf1N1B4_4551 are separated by 26 nucleotides Pf1N1B4_4551 and Pf1N1B4_4552 overlap by 4 nucleotides
Pf1N1B4_4550 - beta-ketoacyl synthase, putative, at 5,018,349 to 5,019,431
_4550
Pf1N1B4_4551 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASII (EC 2.3.1.179), at 5,019,458 to 5,020,723
_4551
Pf1N1B4_4552 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASII (EC 2.3.1.41), at 5,020,720 to 5,021,940
_4552
Position (kb) 5019
5020
5021 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5018.572 kb on - strand, within Pf1N1B4_4550 at 5018.657 kb on - strand, within Pf1N1B4_4550 at 5018.716 kb on + strand, within Pf1N1B4_4550 at 5018.738 kb on + strand, within Pf1N1B4_4550 at 5018.739 kb on - strand, within Pf1N1B4_4550 at 5018.930 kb on + strand, within Pf1N1B4_4550 at 5018.950 kb on - strand at 5020.076 kb on + strand at 5020.268 kb on + strand, within Pf1N1B4_4551 at 5020.559 kb on - strand, within Pf1N1B4_4551 at 5020.610 kb on + strand at 5020.610 kb on + strand at 5020.706 kb on + strand at 5020.707 kb on - strand at 5020.707 kb on - strand at 5020.708 kb on + strand at 5020.709 kb on - strand at 5021.205 kb on + strand, within Pf1N1B4_4552 at 5021.205 kb on + strand, within Pf1N1B4_4552 at 5021.205 kb on + strand, within Pf1N1B4_4552 at 5021.260 kb on + strand, within Pf1N1B4_4552 at 5021.260 kb on + strand at 5021.260 kb on + strand, within Pf1N1B4_4552 at 5021.260 kb on + strand, within Pf1N1B4_4552 at 5021.260 kb on + strand, within Pf1N1B4_4552 at 5021.261 kb on - strand, within Pf1N1B4_4552 at 5021.340 kb on + strand, within Pf1N1B4_4552 at 5021.341 kb on - strand, within Pf1N1B4_4552 at 5021.600 kb on + strand, within Pf1N1B4_4552 at 5021.679 kb on + strand, within Pf1N1B4_4552 at 5021.679 kb on + strand, within Pf1N1B4_4552 at 5021.680 kb on - strand at 5021.680 kb on - strand, within Pf1N1B4_4552 at 5021.680 kb on - strand, within Pf1N1B4_4552
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 5,018,572 - Pf1N1B4_4550 0.21 -0.0 5,018,657 - Pf1N1B4_4550 0.28 -1.4 5,018,716 + Pf1N1B4_4550 0.34 -0.0 5,018,738 + Pf1N1B4_4550 0.36 -0.0 5,018,739 - Pf1N1B4_4550 0.36 -1.9 5,018,930 + Pf1N1B4_4550 0.54 -0.0 5,018,950 - -1.4 5,020,076 + -0.0 5,020,268 + Pf1N1B4_4551 0.64 -2.4 5,020,559 - Pf1N1B4_4551 0.87 -0.0 5,020,610 + -0.0 5,020,610 + -1.5 5,020,706 + -0.0 5,020,707 - -0.0 5,020,707 - -1.1 5,020,708 + -0.0 5,020,709 - -1.8 5,021,205 + Pf1N1B4_4552 0.40 -1.7 5,021,205 + Pf1N1B4_4552 0.40 -0.0 5,021,205 + Pf1N1B4_4552 0.40 -1.9 5,021,260 + Pf1N1B4_4552 0.44 -0.0 5,021,260 + -2.7 5,021,260 + Pf1N1B4_4552 0.44 -3.1 5,021,260 + Pf1N1B4_4552 0.44 -0.0 5,021,260 + Pf1N1B4_4552 0.44 -0.0 5,021,261 - Pf1N1B4_4552 0.44 -1.4 5,021,340 + Pf1N1B4_4552 0.51 -2.9 5,021,341 - Pf1N1B4_4552 0.51 -2.4 5,021,600 + Pf1N1B4_4552 0.72 -0.7 5,021,679 + Pf1N1B4_4552 0.79 -0.0 5,021,679 + Pf1N1B4_4552 0.79 -0.0 5,021,680 - -2.3 5,021,680 - Pf1N1B4_4552 0.79 -2.4 5,021,680 - Pf1N1B4_4552 0.79 -1.9
Or see this region's nucleotide sequence