Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4544

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4542 and Pf1N1B4_4543 are separated by 2 nucleotidesPf1N1B4_4543 and Pf1N1B4_4544 are separated by 502 nucleotidesPf1N1B4_4544 and Pf1N1B4_4545 are separated by 88 nucleotidesPf1N1B4_4545 and Pf1N1B4_4546 overlap by 2 nucleotides Pf1N1B4_4542 - IcmF-related protein, at 5,009,265 to 5,012,888 _4542 Pf1N1B4_4543 - IcmF-related protein, at 5,012,891 to 5,013,040 _4543 Pf1N1B4_4544 - Uncharacterized protein ImpA, at 5,013,543 to 5,014,181 _4544 Pf1N1B4_4545 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 5,014,270 to 5,014,758 _4545 Pf1N1B4_4546 - hypothetical protein, at 5,014,757 to 5,015,215 _4546 Position (kb) 5013 5014 5015Strain fitness (log2 ratio) -3 -2 -1 0 1at 5012.799 kb on + strandat 5012.821 kb on + strandat 5012.872 kb on - strandat 5013.790 kb on - strand, within Pf1N1B4_4544at 5014.030 kb on - strand, within Pf1N1B4_4544at 5014.065 kb on + strand, within Pf1N1B4_4544at 5014.179 kb on + strandat 5015.115 kb on + strand, within Pf1N1B4_4546

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
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5,012,799 + -2.5
5,012,821 + -0.0
5,012,872 - -2.0
5,013,790 - Pf1N1B4_4544 0.39 -3.3
5,014,030 - Pf1N1B4_4544 0.76 -0.0
5,014,065 + Pf1N1B4_4544 0.82 -3.1
5,014,179 + -1.5
5,015,115 + Pf1N1B4_4546 0.78 -2.6

Or see this region's nucleotide sequence