Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4540 and Pf1N1B4_4541 are separated by 18 nucleotides Pf1N1B4_4541 and Pf1N1B4_4542 overlap by 4 nucleotides Pf1N1B4_4542 and Pf1N1B4_4543 are separated by 2 nucleotides Pf1N1B4_4543 and Pf1N1B4_4544 are separated by 502 nucleotides
Pf1N1B4_4540 - Uncharacterized protein ImpJ/VasE, at 5,007,151 to 5,008,482
_4540
Pf1N1B4_4541 - Inner membrane protein DotU, at 5,008,501 to 5,009,268
_4541
Pf1N1B4_4542 - IcmF-related protein, at 5,009,265 to 5,012,888
_4542
Pf1N1B4_4543 - IcmF-related protein, at 5,012,891 to 5,013,040
_4543
Pf1N1B4_4544 - Uncharacterized protein ImpA, at 5,013,543 to 5,014,181
_4544
Position (kb) 5009
5010
5011
5012
5013 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 5008.500 kb on + strand at 5008.501 kb on - strand at 5008.501 kb on - strand at 5009.796 kb on + strand, within Pf1N1B4_4542 at 5010.021 kb on + strand at 5010.130 kb on - strand, within Pf1N1B4_4542 at 5010.426 kb on + strand, within Pf1N1B4_4542 at 5010.894 kb on + strand, within Pf1N1B4_4542 at 5010.895 kb on - strand, within Pf1N1B4_4542 at 5011.012 kb on - strand, within Pf1N1B4_4542 at 5011.536 kb on + strand, within Pf1N1B4_4542 at 5012.026 kb on - strand, within Pf1N1B4_4542 at 5012.073 kb on + strand, within Pf1N1B4_4542 at 5012.304 kb on + strand, within Pf1N1B4_4542 at 5012.799 kb on + strand at 5012.821 kb on + strand at 5012.872 kb on - strand at 5013.790 kb on - strand, within Pf1N1B4_4544
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 5,008,500 + -0.0 5,008,501 - -1.4 5,008,501 - -5.6 5,009,796 + Pf1N1B4_4542 0.15 -1.8 5,010,021 + -1.8 5,010,130 - Pf1N1B4_4542 0.24 -2.2 5,010,426 + Pf1N1B4_4542 0.32 -0.0 5,010,894 + Pf1N1B4_4542 0.45 -0.0 5,010,895 - Pf1N1B4_4542 0.45 -0.0 5,011,012 - Pf1N1B4_4542 0.48 -2.9 5,011,536 + Pf1N1B4_4542 0.63 -0.0 5,012,026 - Pf1N1B4_4542 0.76 -0.0 5,012,073 + Pf1N1B4_4542 0.77 -1.9 5,012,304 + Pf1N1B4_4542 0.84 -0.7 5,012,799 + -2.5 5,012,821 + -0.0 5,012,872 - -2.0 5,013,790 - Pf1N1B4_4544 0.39 -3.3
Or see this region's nucleotide sequence