Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4538 and Pf1N1B4_4539 are separated by 228 nucleotides Pf1N1B4_4539 and Pf1N1B4_4540 are separated by 136 nucleotides Pf1N1B4_4540 and Pf1N1B4_4541 are separated by 18 nucleotides Pf1N1B4_4541 and Pf1N1B4_4542 overlap by 4 nucleotides
Pf1N1B4_4538 - Uncharacterized protein ImpB, at 5,005,788 to 5,006,336
_4538
Pf1N1B4_4539 - Uncharacterized protein homologous to Paeru. PA2364, at 5,006,565 to 5,007,014
_4539
Pf1N1B4_4540 - Uncharacterized protein ImpJ/VasE, at 5,007,151 to 5,008,482
_4540
Pf1N1B4_4541 - Inner membrane protein DotU, at 5,008,501 to 5,009,268
_4541
Pf1N1B4_4542 - IcmF-related protein, at 5,009,265 to 5,012,888
_4542
Position (kb) 5007
5008
5009 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 5006.275 kb on + strand, within Pf1N1B4_4538 at 5006.403 kb on - strand at 5006.511 kb on - strand at 5006.910 kb on + strand, within Pf1N1B4_4539 at 5006.910 kb on + strand at 5006.910 kb on + strand, within Pf1N1B4_4539 at 5006.910 kb on + strand, within Pf1N1B4_4539 at 5006.910 kb on + strand at 5006.911 kb on - strand, within Pf1N1B4_4539 at 5006.911 kb on - strand, within Pf1N1B4_4539 at 5007.150 kb on + strand at 5007.150 kb on + strand at 5007.151 kb on - strand at 5007.161 kb on + strand at 5007.162 kb on - strand at 5007.268 kb on - strand at 5007.931 kb on + strand at 5007.932 kb on - strand, within Pf1N1B4_4540 at 5007.932 kb on - strand, within Pf1N1B4_4540 at 5008.500 kb on + strand at 5008.501 kb on - strand at 5008.501 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 5,006,275 + Pf1N1B4_4538 0.89 -1.6 5,006,403 - -2.3 5,006,511 - -0.0 5,006,910 + Pf1N1B4_4539 0.77 -0.0 5,006,910 + -0.0 5,006,910 + Pf1N1B4_4539 0.77 -1.5 5,006,910 + Pf1N1B4_4539 0.77 -1.5 5,006,910 + +0.6 5,006,911 - Pf1N1B4_4539 0.77 -0.0 5,006,911 - Pf1N1B4_4539 0.77 -0.0 5,007,150 + -1.1 5,007,150 + -0.0 5,007,151 - -0.0 5,007,161 + -3.2 5,007,162 - -0.0 5,007,268 - -0.0 5,007,931 + -0.0 5,007,932 - Pf1N1B4_4540 0.59 -3.5 5,007,932 - Pf1N1B4_4540 0.59 -3.0 5,008,500 + -0.0 5,008,501 - -1.4 5,008,501 - -5.6
Or see this region's nucleotide sequence