Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4488 and Pf1N1B4_4489 are separated by 127 nucleotides Pf1N1B4_4489 and Pf1N1B4_4490 are separated by 57 nucleotides Pf1N1B4_4490 and Pf1N1B4_4491 are separated by 267 nucleotides
Pf1N1B4_4488 - Sensory box histidine kinase/response regulator, at 4,949,952 to 4,952,546
_4488
Pf1N1B4_4489 - Transcriptional regulator, LuxR family, at 4,952,674 to 4,953,339
_4489
Pf1N1B4_4490 - Rhodanese-related sulfurtransferase, at 4,953,397 to 4,953,954
_4490
Pf1N1B4_4491 - ABC transporter permease protein, at 4,954,222 to 4,955,013
_4491
Position (kb)
4952
4953
4954 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4951.713 kb on + strand, within Pf1N1B4_4488 at 4951.842 kb on + strand, within Pf1N1B4_4488 at 4952.304 kb on + strand at 4952.433 kb on + strand at 4952.538 kb on - strand at 4952.907 kb on + strand, within Pf1N1B4_4489 at 4952.966 kb on + strand, within Pf1N1B4_4489 at 4953.051 kb on - strand, within Pf1N1B4_4489 at 4953.376 kb on + strand at 4953.486 kb on + strand, within Pf1N1B4_4490 at 4953.487 kb on - strand, within Pf1N1B4_4490 at 4953.487 kb on - strand, within Pf1N1B4_4490 at 4953.493 kb on + strand, within Pf1N1B4_4490 at 4953.970 kb on + strand at 4953.970 kb on + strand at 4953.971 kb on - strand at 4953.971 kb on - strand at 4953.971 kb on - strand at 4953.971 kb on - strand at 4953.975 kb on - strand at 4954.028 kb on + strand at 4954.036 kb on + strand at 4954.039 kb on + strand at 4954.039 kb on + strand at 4954.040 kb on - strand at 4954.044 kb on + strand at 4954.099 kb on + strand at 4954.099 kb on + strand at 4954.224 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 4,951,713 + Pf1N1B4_4488 0.68 -1.5 4,951,842 + Pf1N1B4_4488 0.73 -1.4 4,952,304 + -2.0 4,952,433 + -0.0 4,952,538 - -0.0 4,952,907 + Pf1N1B4_4489 0.35 -0.0 4,952,966 + Pf1N1B4_4489 0.44 -2.5 4,953,051 - Pf1N1B4_4489 0.57 -2.3 4,953,376 + -1.1 4,953,486 + Pf1N1B4_4490 0.16 -2.0 4,953,487 - Pf1N1B4_4490 0.16 -0.0 4,953,487 - Pf1N1B4_4490 0.16 -1.4 4,953,493 + Pf1N1B4_4490 0.17 -3.2 4,953,970 + -1.8 4,953,970 + -0.0 4,953,971 - -3.0 4,953,971 - -1.3 4,953,971 - -2.5 4,953,971 - -0.0 4,953,975 - -0.0 4,954,028 + -1.9 4,954,036 + -0.0 4,954,039 + -0.0 4,954,039 + -0.0 4,954,040 - -0.0 4,954,044 + -0.0 4,954,099 + -2.1 4,954,099 + -0.0 4,954,224 - -0.0
Or see this region's nucleotide sequence