Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4456 and Pf1N1B4_4457 overlap by 14 nucleotides Pf1N1B4_4457 and Pf1N1B4_4458 overlap by 4 nucleotides Pf1N1B4_4458 and Pf1N1B4_4459 overlap by 11 nucleotides Pf1N1B4_4459 and Pf1N1B4_4460 are separated by 187 nucleotides
Pf1N1B4_4456 - Short-chain dehydrogenase/reductase SDR, at 4,916,582 to 4,917,391
_4456
Pf1N1B4_4457 - long-chain acyl-CoA synthetase, at 4,917,378 to 4,918,052
_4457
Pf1N1B4_4458 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 4,918,049 to 4,919,530
_4458
Pf1N1B4_4459 - Thermostable hemolysin delta-VPH, at 4,919,520 to 4,920,197
_4459
Pf1N1B4_4460 - Cytochrome b561, at 4,920,385 to 4,920,957
_4460
Position (kb)
4918
4919
4920 Strain fitness (log2 ratio)
-2
-1
0
1 at 4917.185 kb on + strand at 4917.436 kb on + strand at 4917.437 kb on - strand at 4917.446 kb on - strand, within Pf1N1B4_4457 at 4917.562 kb on + strand, within Pf1N1B4_4457 at 4917.563 kb on - strand, within Pf1N1B4_4457 at 4917.812 kb on - strand, within Pf1N1B4_4457 at 4917.826 kb on + strand, within Pf1N1B4_4457 at 4917.826 kb on + strand, within Pf1N1B4_4457 at 4917.827 kb on - strand, within Pf1N1B4_4457 at 4917.919 kb on + strand, within Pf1N1B4_4457 at 4917.920 kb on - strand, within Pf1N1B4_4457 at 4917.920 kb on - strand, within Pf1N1B4_4457 at 4918.429 kb on - strand, within Pf1N1B4_4458 at 4918.662 kb on + strand, within Pf1N1B4_4458 at 4918.662 kb on + strand at 4918.663 kb on - strand, within Pf1N1B4_4458 at 4919.095 kb on + strand, within Pf1N1B4_4458 at 4919.430 kb on + strand at 4919.430 kb on + strand at 4919.431 kb on - strand at 4919.648 kb on - strand, within Pf1N1B4_4459 at 4919.797 kb on + strand, within Pf1N1B4_4459 at 4919.797 kb on + strand at 4919.797 kb on + strand at 4919.798 kb on - strand, within Pf1N1B4_4459 at 4920.235 kb on - strand at 4920.285 kb on - strand at 4920.384 kb on + strand at 4920.384 kb on + strand at 4920.385 kb on - strand at 4920.404 kb on + strand at 4920.404 kb on + strand at 4920.469 kb on + strand, within Pf1N1B4_4460
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 4,917,185 + -1.7 4,917,436 + -0.0 4,917,437 - -0.0 4,917,446 - Pf1N1B4_4457 0.10 -0.0 4,917,562 + Pf1N1B4_4457 0.27 -0.0 4,917,563 - Pf1N1B4_4457 0.27 -2.5 4,917,812 - Pf1N1B4_4457 0.64 -0.0 4,917,826 + Pf1N1B4_4457 0.66 -0.0 4,917,826 + Pf1N1B4_4457 0.66 -0.0 4,917,827 - Pf1N1B4_4457 0.67 -1.7 4,917,919 + Pf1N1B4_4457 0.80 -0.0 4,917,920 - Pf1N1B4_4457 0.80 -2.3 4,917,920 - Pf1N1B4_4457 0.80 -1.5 4,918,429 - Pf1N1B4_4458 0.26 -0.0 4,918,662 + Pf1N1B4_4458 0.41 -2.6 4,918,662 + -0.0 4,918,663 - Pf1N1B4_4458 0.41 -0.0 4,919,095 + Pf1N1B4_4458 0.71 -2.2 4,919,430 + -0.0 4,919,430 + -0.0 4,919,431 - -0.0 4,919,648 - Pf1N1B4_4459 0.19 -1.1 4,919,797 + Pf1N1B4_4459 0.41 -2.5 4,919,797 + -0.0 4,919,797 + -2.0 4,919,798 - Pf1N1B4_4459 0.41 -2.0 4,920,235 - -2.0 4,920,285 - -0.8 4,920,384 + -0.0 4,920,384 + -0.0 4,920,385 - -1.2 4,920,404 + -2.7 4,920,404 + -0.0 4,920,469 + Pf1N1B4_4460 0.15 -1.7
Or see this region's nucleotide sequence