Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4304 and Pf1N1B4_4305 are separated by 37 nucleotides Pf1N1B4_4305 and Pf1N1B4_4306 are separated by 145 nucleotides Pf1N1B4_4306 and Pf1N1B4_4307 are separated by 153 nucleotides Pf1N1B4_4307 and Pf1N1B4_4308 are separated by 13 nucleotides Pf1N1B4_4308 and Pf1N1B4_4309 are separated by 273 nucleotides
Pf1N1B4_4304 - hypothetical protein, at 4,767,302 to 4,767,574
_4304
Pf1N1B4_4305 - PUTATIVE METHYLTRANSFERASE, at 4,767,612 to 4,768,424
_4305
Pf1N1B4_4306 - Universal stress protein family 5, at 4,768,570 to 4,769,496
_4306
Pf1N1B4_4307 - Threonine dehydrogenase and related Zn-dependent dehydrogenases, at 4,769,650 to 4,770,039
_4307
Pf1N1B4_4308 - Threonine dehydrogenase and related Zn-dependent dehydrogenases, at 4,770,053 to 4,770,202
_4308
Pf1N1B4_4309 - Translation elongation factor Tu, at 4,770,476 to 4,771,393
_4309
Position (kb) 4768
4769
4770 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 4767.618 kb on - strand at 4767.692 kb on + strand at 4767.700 kb on + strand, within Pf1N1B4_4305 at 4767.700 kb on + strand, within Pf1N1B4_4305 at 4767.701 kb on - strand, within Pf1N1B4_4305 at 4767.701 kb on - strand, within Pf1N1B4_4305 at 4767.834 kb on + strand, within Pf1N1B4_4305 at 4767.834 kb on + strand, within Pf1N1B4_4305 at 4768.266 kb on + strand, within Pf1N1B4_4305 at 4768.435 kb on + strand at 4768.435 kb on + strand at 4768.435 kb on + strand at 4768.435 kb on + strand at 4768.435 kb on + strand at 4768.435 kb on + strand at 4768.435 kb on + strand at 4768.435 kb on + strand at 4768.435 kb on + strand at 4768.436 kb on - strand at 4768.436 kb on - strand at 4768.436 kb on - strand at 4768.436 kb on - strand at 4768.436 kb on - strand at 4768.436 kb on - strand at 4768.436 kb on - strand at 4768.436 kb on - strand at 4768.436 kb on - strand at 4768.436 kb on - strand at 4768.436 kb on - strand at 4768.436 kb on - strand at 4768.436 kb on - strand at 4768.592 kb on + strand at 4768.593 kb on - strand at 4768.706 kb on + strand, within Pf1N1B4_4306 at 4768.707 kb on - strand, within Pf1N1B4_4306 at 4768.707 kb on - strand, within Pf1N1B4_4306 at 4768.707 kb on - strand, within Pf1N1B4_4306 at 4768.707 kb on - strand, within Pf1N1B4_4306 at 4768.707 kb on - strand, within Pf1N1B4_4306 at 4768.707 kb on - strand, within Pf1N1B4_4306 at 4768.707 kb on - strand, within Pf1N1B4_4306 at 4768.711 kb on - strand, within Pf1N1B4_4306 at 4768.907 kb on + strand, within Pf1N1B4_4306 at 4768.907 kb on + strand at 4768.908 kb on - strand, within Pf1N1B4_4306 at 4768.913 kb on + strand at 4768.913 kb on + strand, within Pf1N1B4_4306 at 4768.991 kb on + strand, within Pf1N1B4_4306 at 4769.307 kb on - strand, within Pf1N1B4_4306 at 4769.388 kb on + strand, within Pf1N1B4_4306 at 4769.556 kb on - strand at 4769.560 kb on - strand at 4769.763 kb on + strand, within Pf1N1B4_4307 at 4769.763 kb on + strand, within Pf1N1B4_4307 at 4769.763 kb on + strand, within Pf1N1B4_4307 at 4769.764 kb on - strand, within Pf1N1B4_4307 at 4769.764 kb on - strand, within Pf1N1B4_4307 at 4769.764 kb on - strand, within Pf1N1B4_4307 at 4769.800 kb on + strand, within Pf1N1B4_4307 at 4769.931 kb on + strand, within Pf1N1B4_4307 at 4769.932 kb on - strand, within Pf1N1B4_4307 at 4769.951 kb on + strand, within Pf1N1B4_4307 at 4769.952 kb on - strand, within Pf1N1B4_4307 at 4769.952 kb on - strand, within Pf1N1B4_4307 at 4770.370 kb on - strand at 4770.436 kb on - strand at 4770.478 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 4,767,618 - -1.4 4,767,692 + -0.0 4,767,700 + Pf1N1B4_4305 0.11 -3.0 4,767,700 + Pf1N1B4_4305 0.11 -0.0 4,767,701 - Pf1N1B4_4305 0.11 -1.4 4,767,701 - Pf1N1B4_4305 0.11 -3.8 4,767,834 + Pf1N1B4_4305 0.27 -0.0 4,767,834 + Pf1N1B4_4305 0.27 -0.0 4,768,266 + Pf1N1B4_4305 0.80 -0.0 4,768,435 + -1.4 4,768,435 + -0.0 4,768,435 + -0.0 4,768,435 + -2.1 4,768,435 + -0.0 4,768,435 + -0.0 4,768,435 + -0.0 4,768,435 + -0.0 4,768,435 + -0.0 4,768,436 - -2.0 4,768,436 - -2.1 4,768,436 - -2.7 4,768,436 - -0.0 4,768,436 - -0.0 4,768,436 - -0.0 4,768,436 - -1.6 4,768,436 - -0.0 4,768,436 - -1.4 4,768,436 - -1.7 4,768,436 - -0.0 4,768,436 - -2.9 4,768,436 - -1.4 4,768,592 + -2.6 4,768,593 - -1.4 4,768,706 + Pf1N1B4_4306 0.15 -1.9 4,768,707 - Pf1N1B4_4306 0.15 -2.1 4,768,707 - Pf1N1B4_4306 0.15 -1.5 4,768,707 - Pf1N1B4_4306 0.15 -0.0 4,768,707 - Pf1N1B4_4306 0.15 -1.2 4,768,707 - Pf1N1B4_4306 0.15 -0.0 4,768,707 - Pf1N1B4_4306 0.15 -2.1 4,768,707 - Pf1N1B4_4306 0.15 -0.4 4,768,711 - Pf1N1B4_4306 0.15 -0.0 4,768,907 + Pf1N1B4_4306 0.36 -1.5 4,768,907 + -2.4 4,768,908 - Pf1N1B4_4306 0.36 -0.0 4,768,913 + -0.2 4,768,913 + Pf1N1B4_4306 0.37 -0.0 4,768,991 + Pf1N1B4_4306 0.45 -0.0 4,769,307 - Pf1N1B4_4306 0.80 -0.0 4,769,388 + Pf1N1B4_4306 0.88 -2.4 4,769,556 - -0.0 4,769,560 - -0.0 4,769,763 + Pf1N1B4_4307 0.29 -0.0 4,769,763 + Pf1N1B4_4307 0.29 -0.0 4,769,763 + Pf1N1B4_4307 0.29 -0.0 4,769,764 - Pf1N1B4_4307 0.29 -1.6 4,769,764 - Pf1N1B4_4307 0.29 -0.0 4,769,764 - Pf1N1B4_4307 0.29 -1.4 4,769,800 + Pf1N1B4_4307 0.38 -1.6 4,769,931 + Pf1N1B4_4307 0.72 -1.0 4,769,932 - Pf1N1B4_4307 0.72 -0.0 4,769,951 + Pf1N1B4_4307 0.77 -2.3 4,769,952 - Pf1N1B4_4307 0.77 -0.0 4,769,952 - Pf1N1B4_4307 0.77 -0.0 4,770,370 - -0.0 4,770,436 - -0.0 4,770,478 - -0.0
Or see this region's nucleotide sequence