Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_429 and Pf1N1B4_430 are separated by 666 nucleotides
Pf1N1B4_429 - FIG00964214: hypothetical protein, at 499,404 to 499,703
_429
Pf1N1B4_430 - hypothetical protein, at 500,370 to 500,486
_430
Position (kb) 500
501 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 499.627 kb on + strand, within Pf1N1B4_429 at 499.628 kb on - strand, within Pf1N1B4_429 at 499.655 kb on + strand, within Pf1N1B4_429 at 499.655 kb on + strand, within Pf1N1B4_429 at 499.655 kb on + strand, within Pf1N1B4_429 at 499.656 kb on - strand, within Pf1N1B4_429 at 499.737 kb on - strand at 499.750 kb on + strand at 499.750 kb on + strand at 499.763 kb on - strand at 499.788 kb on + strand at 499.789 kb on - strand at 499.789 kb on - strand at 499.863 kb on + strand at 499.903 kb on + strand at 499.903 kb on + strand at 499.904 kb on - strand at 499.904 kb on - strand at 499.962 kb on + strand at 499.962 kb on + strand at 499.962 kb on + strand at 499.962 kb on + strand at 499.963 kb on - strand at 499.972 kb on + strand at 500.094 kb on + strand at 500.094 kb on + strand at 500.094 kb on + strand at 500.095 kb on - strand at 500.095 kb on - strand at 500.104 kb on - strand at 500.111 kb on + strand at 500.129 kb on - strand at 500.235 kb on - strand at 500.338 kb on + strand at 500.386 kb on + strand, within Pf1N1B4_430 at 500.387 kb on - strand, within Pf1N1B4_430 at 500.387 kb on - strand, within Pf1N1B4_430 at 500.416 kb on - strand, within Pf1N1B4_430 at 500.416 kb on - strand, within Pf1N1B4_430 at 500.515 kb on + strand at 500.560 kb on + strand at 500.613 kb on + strand at 500.615 kb on + strand at 500.615 kb on + strand at 500.615 kb on + strand at 500.615 kb on + strand at 500.615 kb on + strand at 500.615 kb on + strand at 500.615 kb on + strand at 500.616 kb on - strand at 500.616 kb on - strand at 500.616 kb on - strand at 500.831 kb on + strand at 500.850 kb on - strand at 500.903 kb on - strand at 500.909 kb on - strand at 500.909 kb on - strand at 500.909 kb on - strand at 500.909 kb on - strand at 500.913 kb on - strand at 500.913 kb on - strand at 500.990 kb on - strand at 501.035 kb on - strand at 501.035 kb on - strand at 501.035 kb on - strand at 501.043 kb on + strand at 501.043 kb on + strand at 501.043 kb on + strand at 501.044 kb on - strand at 501.044 kb on - strand at 501.047 kb on + strand at 501.047 kb on + strand at 501.047 kb on + strand at 501.047 kb on + strand at 501.047 kb on + strand at 501.047 kb on + strand at 501.047 kb on + strand at 501.048 kb on - strand at 501.048 kb on - strand at 501.059 kb on + strand at 501.060 kb on - strand at 501.094 kb on + strand at 501.151 kb on + strand at 501.152 kb on - strand at 501.190 kb on - strand at 501.190 kb on - strand at 501.197 kb on - strand at 501.197 kb on - strand at 501.246 kb on + strand at 501.251 kb on + strand at 501.251 kb on + strand at 501.251 kb on + strand at 501.251 kb on + strand at 501.251 kb on + strand at 501.251 kb on + strand at 501.251 kb on + strand at 501.252 kb on - strand at 501.252 kb on - strand at 501.252 kb on - strand at 501.252 kb on - strand at 501.252 kb on - strand at 501.252 kb on - strand at 501.252 kb on - strand at 501.463 kb on - strand at 501.463 kb on - strand at 501.463 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 499,627 + Pf1N1B4_429 0.74 -1.6 499,628 - Pf1N1B4_429 0.75 -0.0 499,655 + Pf1N1B4_429 0.84 -0.0 499,655 + Pf1N1B4_429 0.84 -1.2 499,655 + Pf1N1B4_429 0.84 -0.0 499,656 - Pf1N1B4_429 0.84 -0.0 499,737 - -0.0 499,750 + -0.0 499,750 + -0.0 499,763 - -1.9 499,788 + -0.0 499,789 - -1.1 499,789 - -0.0 499,863 + -2.0 499,903 + -0.0 499,903 + -0.0 499,904 - -2.4 499,904 - -0.0 499,962 + -3.9 499,962 + -0.0 499,962 + -0.0 499,962 + -1.6 499,963 - -0.0 499,972 + -0.0 500,094 + -1.4 500,094 + -1.6 500,094 + -0.0 500,095 - -2.1 500,095 - -0.0 500,104 - -0.0 500,111 + -2.4 500,129 - -3.4 500,235 - -0.0 500,338 + -0.0 500,386 + Pf1N1B4_430 0.14 -0.0 500,387 - Pf1N1B4_430 0.15 -2.2 500,387 - Pf1N1B4_430 0.15 -3.1 500,416 - Pf1N1B4_430 0.39 -0.0 500,416 - Pf1N1B4_430 0.39 -0.0 500,515 + -0.0 500,560 + -1.2 500,613 + -0.0 500,615 + -0.0 500,615 + -0.0 500,615 + -0.0 500,615 + -0.0 500,615 + -2.8 500,615 + -0.0 500,615 + -3.3 500,616 - -0.0 500,616 - -1.7 500,616 - -0.0 500,831 + -0.0 500,850 - -2.6 500,903 - -0.0 500,909 - -0.0 500,909 - -2.0 500,909 - -0.0 500,909 - -1.9 500,913 - -0.0 500,913 - -1.5 500,990 - -1.2 501,035 - -0.0 501,035 - -1.3 501,035 - -0.0 501,043 + -0.0 501,043 + -0.0 501,043 + -0.0 501,044 - -2.9 501,044 - -0.0 501,047 + -0.0 501,047 + -1.0 501,047 + -1.2 501,047 + -0.0 501,047 + -0.0 501,047 + -2.7 501,047 + -0.0 501,048 - -0.0 501,048 - -0.0 501,059 + -1.6 501,060 - -0.0 501,094 + -0.0 501,151 + -2.5 501,152 - -1.8 501,190 - -0.0 501,190 - -1.8 501,197 - -0.0 501,197 - -0.0 501,246 + -1.5 501,251 + -0.0 501,251 + -1.9 501,251 + -0.0 501,251 + -0.0 501,251 + -0.0 501,251 + -0.0 501,251 + -0.0 501,252 - -0.0 501,252 - -1.7 501,252 - -0.0 501,252 - -0.0 501,252 - -1.4 501,252 - -1.3 501,252 - -0.0 501,463 - -1.4 501,463 - -0.0 501,463 - -2.2
Or see this region's nucleotide sequence