Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4253 and Pf1N1B4_4254 are separated by 448 nucleotides Pf1N1B4_4254 and Pf1N1B4_4255 are separated by 75 nucleotides Pf1N1B4_4255 and Pf1N1B4_4256 are separated by 106 nucleotides
Pf1N1B4_4253 - RNA polymerase sigma-70 factor, ECF subfamily, at 4,716,258 to 4,716,791
_4253
Pf1N1B4_4254 - Sensory box histidine kinase/response regulator, at 4,717,240 to 4,717,617
_4254
Pf1N1B4_4255 - Putative phosphatase YfbT, at 4,717,693 to 4,718,361
_4255
Pf1N1B4_4256 - Probable transmembrane protein, at 4,718,468 to 4,719,148
_4256
Position (kb)
4717
4718
4719 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4717.075 kb on + strand at 4717.115 kb on - strand at 4717.149 kb on + strand at 4717.150 kb on - strand at 4717.150 kb on - strand at 4717.150 kb on - strand at 4717.155 kb on + strand at 4717.156 kb on - strand at 4717.651 kb on + strand at 4717.651 kb on + strand at 4717.651 kb on + strand at 4717.651 kb on + strand at 4717.652 kb on - strand at 4717.652 kb on - strand at 4717.652 kb on - strand at 4717.856 kb on + strand, within Pf1N1B4_4255 at 4717.857 kb on - strand, within Pf1N1B4_4255 at 4717.872 kb on + strand, within Pf1N1B4_4255 at 4717.872 kb on + strand, within Pf1N1B4_4255 at 4717.873 kb on - strand, within Pf1N1B4_4255 at 4718.302 kb on + strand at 4718.351 kb on - strand at 4718.351 kb on - strand at 4718.359 kb on + strand at 4718.360 kb on - strand at 4718.360 kb on - strand at 4718.453 kb on + strand at 4718.453 kb on + strand at 4718.454 kb on - strand at 4718.454 kb on - strand at 4718.473 kb on - strand at 4718.497 kb on + strand at 4718.498 kb on - strand at 4718.517 kb on + strand at 4718.518 kb on - strand at 4718.518 kb on - strand at 4718.541 kb on - strand, within Pf1N1B4_4256 at 4718.664 kb on + strand at 4718.664 kb on + strand, within Pf1N1B4_4256 at 4718.861 kb on - strand, within Pf1N1B4_4256 at 4718.862 kb on + strand, within Pf1N1B4_4256 at 4718.863 kb on - strand, within Pf1N1B4_4256 at 4719.076 kb on - strand, within Pf1N1B4_4256 at 4719.150 kb on + strand at 4719.150 kb on + strand at 4719.150 kb on + strand at 4719.150 kb on + strand at 4719.150 kb on + strand at 4719.150 kb on + strand at 4719.150 kb on + strand at 4719.150 kb on + strand at 4719.151 kb on - strand at 4719.151 kb on - strand at 4719.151 kb on - strand at 4719.151 kb on - strand at 4719.151 kb on - strand at 4719.151 kb on - strand at 4719.151 kb on - strand at 4719.225 kb on - strand at 4719.288 kb on + strand at 4719.288 kb on + strand at 4719.288 kb on + strand at 4719.289 kb on - strand at 4719.289 kb on - strand at 4719.289 kb on - strand at 4719.314 kb on + strand at 4719.314 kb on + strand at 4719.314 kb on + strand at 4719.314 kb on + strand at 4719.315 kb on - strand at 4719.315 kb on - strand at 4719.315 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 4,717,075 + -0.0 4,717,115 - -2.1 4,717,149 + -0.0 4,717,150 - -0.0 4,717,150 - -2.3 4,717,150 - -0.0 4,717,155 + -0.0 4,717,156 - -0.0 4,717,651 + -1.8 4,717,651 + -0.6 4,717,651 + -0.0 4,717,651 + -1.6 4,717,652 - -0.0 4,717,652 - -4.4 4,717,652 - -0.0 4,717,856 + Pf1N1B4_4255 0.24 -2.0 4,717,857 - Pf1N1B4_4255 0.25 -2.9 4,717,872 + Pf1N1B4_4255 0.27 -2.5 4,717,872 + Pf1N1B4_4255 0.27 -0.0 4,717,873 - Pf1N1B4_4255 0.27 -0.0 4,718,302 + -0.0 4,718,351 - -0.0 4,718,351 - -0.9 4,718,359 + -1.7 4,718,360 - -0.0 4,718,360 - -0.0 4,718,453 + -0.0 4,718,453 + -2.3 4,718,454 - -0.3 4,718,454 - -1.6 4,718,473 - -0.0 4,718,497 + -2.1 4,718,498 - -1.1 4,718,517 + -0.0 4,718,518 - -0.0 4,718,518 - -0.0 4,718,541 - Pf1N1B4_4256 0.11 -0.0 4,718,664 + -0.0 4,718,664 + Pf1N1B4_4256 0.29 -1.8 4,718,861 - Pf1N1B4_4256 0.58 -0.0 4,718,862 + Pf1N1B4_4256 0.58 -0.9 4,718,863 - Pf1N1B4_4256 0.58 -0.0 4,719,076 - Pf1N1B4_4256 0.89 -2.3 4,719,150 + -0.0 4,719,150 + -1.9 4,719,150 + -1.8 4,719,150 + -0.0 4,719,150 + -1.8 4,719,150 + -1.0 4,719,150 + -0.0 4,719,150 + -0.0 4,719,151 - -0.8 4,719,151 - -0.0 4,719,151 - -0.0 4,719,151 - -1.0 4,719,151 - -0.0 4,719,151 - -0.0 4,719,151 - -0.0 4,719,225 - -0.0 4,719,288 + -1.9 4,719,288 + -3.6 4,719,288 + -2.7 4,719,289 - -2.3 4,719,289 - -0.0 4,719,289 - -2.0 4,719,314 + -3.4 4,719,314 + -2.2 4,719,314 + -1.5 4,719,314 + -0.0 4,719,315 - -0.0 4,719,315 - -0.0 4,719,315 - -0.0
Or see this region's nucleotide sequence