Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4201

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
4,652,215	+		Pf1N1B4_4200	0.34	+0.0
4,652,215	+		Pf1N1B4_4200	0.34	+0.0
4,652,216	-				-2.1
4,652,298	+		Pf1N1B4_4200	0.41	-1.5
4,652,484	+		Pf1N1B4_4200	0.56	+0.0
4,652,485	-		Pf1N1B4_4200	0.56	+0.0
4,652,485	-		Pf1N1B4_4200	0.56	+0.0
4,652,636	+		Pf1N1B4_4200	0.68	-1.7
4,652,643	+				-1.1
4,652,727	+		Pf1N1B4_4200	0.75	-1.1
4,652,728	-		Pf1N1B4_4200	0.75	+0.0
4,652,728	-				-1.8
4,652,740	-		Pf1N1B4_4200	0.76	-2.5
4,652,829	+		Pf1N1B4_4200	0.83	-2.1
4,653,166	+				-1.3
4,653,485	+		Pf1N1B4_4201	0.38	+0.0
4,653,486	-		Pf1N1B4_4201	0.39	+0.0
4,653,486	-		Pf1N1B4_4201	0.39	-3.0
4,653,585	+		Pf1N1B4_4201	0.47	+0.0
4,653,586	-				-1.1
4,654,281	+				-2.0
4,654,501	-		Pf1N1B4_4202	0.14	-2.2
4,654,768	-		Pf1N1B4_4202	0.50	-1.3
4,654,836	+		Pf1N1B4_4202	0.59	+0.0
4,654,837	-		Pf1N1B4_4202	0.59	+0.0
4,654,839	-		Pf1N1B4_4202	0.60	-1.6
4,654,855	+				+0.0
4,654,959	+		Pf1N1B4_4202	0.76	-2.6
4,655,004	+		Pf1N1B4_4202	0.82	-3.3
4,655,004	+		Pf1N1B4_4202	0.82	+0.0
4,655,007	+		Pf1N1B4_4202	0.82	+0.0
4,655,007	+				+0.0
4,655,048	+		Pf1N1B4_4202	0.88	-2.4
4,655,049	-		Pf1N1B4_4202	0.88	-1.3
4,655,082	+				+0.0
4,655,082	+				+0.0
4,655,095	-				+0.0
4,655,173	+				+0.0
4,655,174	-				-0.8