Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4178 and Pf1N1B4_4179 are separated by 154 nucleotides Pf1N1B4_4179 and Pf1N1B4_4180 are separated by 163 nucleotides Pf1N1B4_4180 and Pf1N1B4_4181 are separated by 45 nucleotides
Pf1N1B4_4178 - Cell division protein FtsH (EC 3.4.24.-), at 4,626,588 to 4,628,375
_4178
Pf1N1B4_4179 - phosphoribosyltransferase, at 4,628,530 to 4,629,114
_4179
Pf1N1B4_4180 - Phosphoribosyl transferase domain protein, at 4,629,278 to 4,629,955
_4180
Pf1N1B4_4181 - FIG00957123: hypothetical protein, at 4,630,001 to 4,630,426
_4181
Position (kb)
4628
4629
4630 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4627.531 kb on - strand at 4627.531 kb on - strand, within Pf1N1B4_4178 at 4627.834 kb on + strand, within Pf1N1B4_4178 at 4627.834 kb on + strand, within Pf1N1B4_4178 at 4628.328 kb on - strand at 4628.391 kb on + strand at 4628.515 kb on + strand at 4628.516 kb on - strand at 4628.521 kb on + strand at 4628.711 kb on + strand, within Pf1N1B4_4179 at 4628.711 kb on + strand, within Pf1N1B4_4179 at 4628.712 kb on - strand, within Pf1N1B4_4179 at 4628.741 kb on + strand, within Pf1N1B4_4179 at 4628.806 kb on - strand at 4628.885 kb on + strand, within Pf1N1B4_4179 at 4628.953 kb on + strand, within Pf1N1B4_4179 at 4628.954 kb on - strand at 4629.149 kb on + strand at 4629.149 kb on + strand at 4629.456 kb on + strand, within Pf1N1B4_4180 at 4629.457 kb on - strand, within Pf1N1B4_4180 at 4629.457 kb on - strand, within Pf1N1B4_4180 at 4629.784 kb on + strand, within Pf1N1B4_4180 at 4629.784 kb on + strand, within Pf1N1B4_4180 at 4629.926 kb on - strand at 4629.966 kb on - strand at 4630.003 kb on - strand at 4630.018 kb on - strand at 4630.064 kb on + strand, within Pf1N1B4_4181 at 4630.065 kb on - strand, within Pf1N1B4_4181
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 4,627,531 - -1.6 4,627,531 - Pf1N1B4_4178 0.53 -1.7 4,627,834 + Pf1N1B4_4178 0.70 +0.0 4,627,834 + Pf1N1B4_4178 0.70 -3.1 4,628,328 - -1.4 4,628,391 + +0.9 4,628,515 + -1.1 4,628,516 - +0.0 4,628,521 + +0.0 4,628,711 + Pf1N1B4_4179 0.31 +0.0 4,628,711 + Pf1N1B4_4179 0.31 -3.1 4,628,712 - Pf1N1B4_4179 0.31 +0.0 4,628,741 + Pf1N1B4_4179 0.36 -2.4 4,628,806 - +0.7 4,628,885 + Pf1N1B4_4179 0.61 -2.9 4,628,953 + Pf1N1B4_4179 0.72 +0.0 4,628,954 - -2.3 4,629,149 + +0.0 4,629,149 + +0.0 4,629,456 + Pf1N1B4_4180 0.26 +0.0 4,629,457 - Pf1N1B4_4180 0.26 +0.0 4,629,457 - Pf1N1B4_4180 0.26 -2.8 4,629,784 + Pf1N1B4_4180 0.75 -1.6 4,629,784 + Pf1N1B4_4180 0.75 +0.0 4,629,926 - +0.0 4,629,966 - +0.0 4,630,003 - +0.0 4,630,018 - +0.0 4,630,064 + Pf1N1B4_4181 0.15 +0.0 4,630,065 - Pf1N1B4_4181 0.15 +0.0
Or see this region's nucleotide sequence