Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_412

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_411 and Pf1N1B4_412 are separated by 122 nucleotidesPf1N1B4_412 and Pf1N1B4_413 are separated by 270 nucleotides Pf1N1B4_411 - ABC transporter for D-galactose/L-arabinose, substrate-binding component (from data), at 480,413 to 481,417 _411 Pf1N1B4_412 - L-arabinose 1-dehydrogenase / D-galactose 1-dehydrogenase (EC 1.1.1.46; EC 1.1.1.48) (from data), at 481,540 to 482,358 _412 Pf1N1B4_413 - L-arabinolactonase (EC 3.1.1.15) (from data), at 482,629 to 483,573 _413 Position (kb) 481 482 483Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 480.549 kb on + strand, within Pf1N1B4_411at 480.549 kb on + strand, within Pf1N1B4_411at 480.886 kb on + strand, within Pf1N1B4_411at 480.906 kb on + strand, within Pf1N1B4_411at 480.907 kb on - strand, within Pf1N1B4_411at 480.907 kb on - strand, within Pf1N1B4_411at 480.924 kb on + strand, within Pf1N1B4_411at 480.924 kb on + strand, within Pf1N1B4_411at 480.925 kb on - strandat 480.925 kb on - strand, within Pf1N1B4_411at 480.925 kb on - strand, within Pf1N1B4_411at 480.925 kb on - strand, within Pf1N1B4_411at 480.925 kb on - strand, within Pf1N1B4_411at 481.345 kb on + strandat 481.345 kb on + strandat 481.345 kb on + strandat 481.346 kb on - strandat 481.346 kb on - strandat 481.346 kb on - strandat 481.346 kb on - strandat 481.346 kb on - strandat 481.346 kb on - strandat 481.346 kb on - strandat 481.417 kb on + strandat 481.418 kb on - strandat 481.418 kb on - strandat 481.450 kb on + strandat 481.537 kb on + strandat 481.550 kb on + strandat 481.550 kb on + strandat 481.550 kb on + strandat 481.550 kb on + strandat 481.551 kb on - strandat 481.593 kb on + strandat 481.796 kb on + strand, within Pf1N1B4_412at 481.796 kb on + strand, within Pf1N1B4_412at 482.087 kb on - strand, within Pf1N1B4_412at 482.123 kb on - strand, within Pf1N1B4_412at 482.505 kb on - strandat 482.538 kb on - strandat 482.538 kb on - strandat 482.570 kb on + strandat 482.572 kb on + strandat 482.573 kb on - strandat 482.600 kb on + strandat 482.600 kb on + strandat 482.601 kb on - strandat 482.668 kb on + strandat 482.668 kb on + strandat 482.668 kb on + strandat 482.668 kb on + strandat 482.668 kb on + strandat 482.668 kb on + strandat 482.668 kb on + strandat 482.669 kb on - strandat 482.669 kb on - strandat 482.732 kb on + strand, within Pf1N1B4_413at 482.732 kb on + strand, within Pf1N1B4_413at 482.732 kb on + strand, within Pf1N1B4_413at 482.732 kb on + strand, within Pf1N1B4_413at 482.733 kb on - strand, within Pf1N1B4_413at 482.810 kb on + strand, within Pf1N1B4_413at 482.811 kb on - strand, within Pf1N1B4_413at 482.863 kb on + strand, within Pf1N1B4_413at 482.863 kb on + strand, within Pf1N1B4_413at 482.863 kb on + strand, within Pf1N1B4_413at 482.863 kb on + strand, within Pf1N1B4_413at 482.863 kb on + strand, within Pf1N1B4_413at 482.863 kb on + strand, within Pf1N1B4_413at 482.863 kb on + strandat 482.863 kb on + strand, within Pf1N1B4_413at 482.864 kb on - strand, within Pf1N1B4_413at 482.864 kb on - strand, within Pf1N1B4_413at 482.864 kb on - strand, within Pf1N1B4_413at 482.864 kb on - strand, within Pf1N1B4_413at 482.864 kb on - strand, within Pf1N1B4_413at 482.864 kb on - strand, within Pf1N1B4_413at 482.864 kb on - strand, within Pf1N1B4_413at 482.864 kb on - strand, within Pf1N1B4_413at 482.864 kb on - strand, within Pf1N1B4_413at 482.867 kb on + strand, within Pf1N1B4_413at 482.867 kb on + strand, within Pf1N1B4_413at 482.867 kb on + strand, within Pf1N1B4_413at 482.867 kb on + strand, within Pf1N1B4_413at 482.868 kb on - strand, within Pf1N1B4_413at 482.868 kb on - strand, within Pf1N1B4_413at 482.868 kb on - strand, within Pf1N1B4_413at 482.868 kb on - strand, within Pf1N1B4_413at 482.868 kb on - strand, within Pf1N1B4_413at 482.868 kb on - strand, within Pf1N1B4_413at 482.868 kb on - strand, within Pf1N1B4_413at 482.930 kb on + strand, within Pf1N1B4_413at 482.930 kb on + strand, within Pf1N1B4_413at 482.930 kb on + strand, within Pf1N1B4_413at 482.931 kb on - strand, within Pf1N1B4_413at 482.931 kb on - strand, within Pf1N1B4_413at 482.931 kb on - strand, within Pf1N1B4_413at 482.931 kb on - strand, within Pf1N1B4_413at 482.931 kb on - strand, within Pf1N1B4_413at 482.931 kb on - strand, within Pf1N1B4_413at 483.002 kb on + strand, within Pf1N1B4_413at 483.003 kb on - strand, within Pf1N1B4_413at 483.049 kb on + strand, within Pf1N1B4_413at 483.049 kb on + strand, within Pf1N1B4_413at 483.050 kb on - strand, within Pf1N1B4_413at 483.058 kb on + strand, within Pf1N1B4_413at 483.058 kb on + strand, within Pf1N1B4_413at 483.059 kb on - strand, within Pf1N1B4_413at 483.059 kb on - strand, within Pf1N1B4_413at 483.059 kb on - strand, within Pf1N1B4_413at 483.061 kb on + strand, within Pf1N1B4_413at 483.124 kb on + strand, within Pf1N1B4_413

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
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480,549 + Pf1N1B4_411 0.14 -0.0
480,549 + Pf1N1B4_411 0.14 -3.0
480,886 + Pf1N1B4_411 0.47 -0.0
480,906 + Pf1N1B4_411 0.49 -1.9
480,907 - Pf1N1B4_411 0.49 -0.0
480,907 - Pf1N1B4_411 0.49 -0.0
480,924 + Pf1N1B4_411 0.51 -0.0
480,924 + Pf1N1B4_411 0.51 -0.0
480,925 - -0.0
480,925 - Pf1N1B4_411 0.51 -3.0
480,925 - Pf1N1B4_411 0.51 -0.0
480,925 - Pf1N1B4_411 0.51 -0.0
480,925 - Pf1N1B4_411 0.51 -0.0
481,345 + -0.0
481,345 + -3.4
481,345 + -2.1
481,346 - -0.0
481,346 - -0.0
481,346 - -3.5
481,346 - -0.2
481,346 - -0.0
481,346 - -0.0
481,346 - -0.0
481,417 + -2.6
481,418 - -1.4
481,418 - -0.0
481,450 + -2.3
481,537 + -0.0
481,550 + -0.0
481,550 + -1.9
481,550 + -0.0
481,550 + -2.3
481,551 - -0.0
481,593 + -1.0
481,796 + Pf1N1B4_412 0.31 -0.0
481,796 + Pf1N1B4_412 0.31 -0.0
482,087 - Pf1N1B4_412 0.67 -2.2
482,123 - Pf1N1B4_412 0.71 -2.0
482,505 - -5.0
482,538 - -0.0
482,538 - -0.0
482,570 + -0.0
482,572 + -0.0
482,573 - -0.0
482,600 + -0.0
482,600 + -1.0
482,601 - -0.0
482,668 + -2.4
482,668 + -1.8
482,668 + -0.0
482,668 + -2.5
482,668 + -1.0
482,668 + -2.7
482,668 + -3.4
482,669 - -0.0
482,669 - -1.0
482,732 + Pf1N1B4_413 0.11 -0.0
482,732 + Pf1N1B4_413 0.11 -0.0
482,732 + Pf1N1B4_413 0.11 -0.0
482,732 + Pf1N1B4_413 0.11 -0.0
482,733 - Pf1N1B4_413 0.11 -0.0
482,810 + Pf1N1B4_413 0.19 -0.0
482,811 - Pf1N1B4_413 0.19 -1.8
482,863 + Pf1N1B4_413 0.25 -2.6
482,863 + Pf1N1B4_413 0.25 -2.0
482,863 + Pf1N1B4_413 0.25 -0.0
482,863 + Pf1N1B4_413 0.25 -2.2
482,863 + Pf1N1B4_413 0.25 -0.0
482,863 + Pf1N1B4_413 0.25 -1.8
482,863 + -0.4
482,863 + Pf1N1B4_413 0.25 -1.6
482,864 - Pf1N1B4_413 0.25 -0.0
482,864 - Pf1N1B4_413 0.25 -2.2
482,864 - Pf1N1B4_413 0.25 -1.9
482,864 - Pf1N1B4_413 0.25 -0.0
482,864 - Pf1N1B4_413 0.25 -1.5
482,864 - Pf1N1B4_413 0.25 -0.0
482,864 - Pf1N1B4_413 0.25 -1.7
482,864 - Pf1N1B4_413 0.25 -1.5
482,864 - Pf1N1B4_413 0.25 -2.6
482,867 + Pf1N1B4_413 0.25 -0.0
482,867 + Pf1N1B4_413 0.25 -0.0
482,867 + Pf1N1B4_413 0.25 -0.0
482,867 + Pf1N1B4_413 0.25 -0.0
482,868 - Pf1N1B4_413 0.25 -0.0
482,868 - Pf1N1B4_413 0.25 -0.0
482,868 - Pf1N1B4_413 0.25 -2.3
482,868 - Pf1N1B4_413 0.25 -0.0
482,868 - Pf1N1B4_413 0.25 -0.0
482,868 - Pf1N1B4_413 0.25 -1.7
482,868 - Pf1N1B4_413 0.25 -0.0
482,930 + Pf1N1B4_413 0.32 -1.3
482,930 + Pf1N1B4_413 0.32 -0.0
482,930 + Pf1N1B4_413 0.32 -0.0
482,931 - Pf1N1B4_413 0.32 -2.4
482,931 - Pf1N1B4_413 0.32 -2.0
482,931 - Pf1N1B4_413 0.32 -2.2
482,931 - Pf1N1B4_413 0.32 -2.4
482,931 - Pf1N1B4_413 0.32 -1.5
482,931 - Pf1N1B4_413 0.32 -1.9
483,002 + Pf1N1B4_413 0.39 -0.0
483,003 - Pf1N1B4_413 0.40 -0.0
483,049 + Pf1N1B4_413 0.44 -0.0
483,049 + Pf1N1B4_413 0.44 -0.0
483,050 - Pf1N1B4_413 0.45 -0.0
483,058 + Pf1N1B4_413 0.45 -2.6
483,058 + Pf1N1B4_413 0.45 -0.0
483,059 - Pf1N1B4_413 0.46 -0.0
483,059 - Pf1N1B4_413 0.46 -2.0
483,059 - Pf1N1B4_413 0.46 -1.7
483,061 + Pf1N1B4_413 0.46 -0.0
483,124 + Pf1N1B4_413 0.52 -0.0

Or see this region's nucleotide sequence