Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4049

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4048 and Pf1N1B4_4049 are separated by 59 nucleotidesPf1N1B4_4049 and Pf1N1B4_4050 are separated by 213 nucleotidesPf1N1B4_4050 and Pf1N1B4_4051 are separated by 38 nucleotides Pf1N1B4_4048 - tRNA-specific 2-thiouridylase MnmA, at 4,500,456 to 4,501,589 _4048 Pf1N1B4_4049 - Nudix-like NDP and NTP phosphohydrolase YmfB, at 4,501,649 to 4,502,095 _4049 Pf1N1B4_4050 - Isocitrate dehydrogenase [NADP] (EC 1.1.1.42); Monomeric isocitrate dehydrogenase [NADP] (EC 1.1.1.42), at 4,502,309 to 4,502,422 _4050 Pf1N1B4_4051 - Isocitrate dehydrogenase [NADP] (EC 1.1.1.42); Monomeric isocitrate dehydrogenase [NADP] (EC 1.1.1.42), at 4,502,461 to 4,504,533 _4051 Position (kb) 4501 4502 4503Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 4501.709 kb on - strandat 4501.709 kb on - strand, within Pf1N1B4_4049at 4501.806 kb on + strand, within Pf1N1B4_4049at 4501.854 kb on + strand, within Pf1N1B4_4049at 4501.854 kb on + strand, within Pf1N1B4_4049at 4501.855 kb on - strand, within Pf1N1B4_4049at 4501.881 kb on + strand, within Pf1N1B4_4049at 4501.881 kb on + strand, within Pf1N1B4_4049at 4501.881 kb on + strand, within Pf1N1B4_4049at 4501.887 kb on + strandat 4501.887 kb on + strand, within Pf1N1B4_4049at 4501.887 kb on + strand, within Pf1N1B4_4049at 4501.887 kb on + strand, within Pf1N1B4_4049at 4501.887 kb on + strand, within Pf1N1B4_4049at 4502.239 kb on + strandat 4502.239 kb on + strandat 4502.240 kb on - strandat 4502.240 kb on - strandat 4502.240 kb on - strandat 4502.240 kb on - strandat 4502.240 kb on - strandat 4502.240 kb on - strandat 4502.240 kb on - strandat 4502.240 kb on - strandat 4502.240 kb on - strandat 4502.240 kb on - strandat 4502.240 kb on - strandat 4502.243 kb on + strandat 4502.243 kb on + strandat 4502.243 kb on + strandat 4502.243 kb on + strandat 4502.243 kb on + strandat 4502.243 kb on + strandat 4502.243 kb on + strandat 4502.243 kb on + strandat 4502.243 kb on + strandat 4502.243 kb on + strandat 4502.243 kb on + strandat 4502.243 kb on + strandat 4502.243 kb on + strandat 4502.243 kb on + strandat 4502.244 kb on - strandat 4502.244 kb on - strandat 4502.244 kb on - strandat 4502.244 kb on - strandat 4502.244 kb on - strandat 4502.247 kb on + strandat 4502.247 kb on + strandat 4502.247 kb on + strandat 4502.247 kb on + strandat 4502.248 kb on - strandat 4502.248 kb on - strandat 4502.302 kb on + strandat 4502.310 kb on + strandat 4502.551 kb on - strandat 4502.663 kb on - strandat 4502.673 kb on + strand, within Pf1N1B4_4051at 4502.674 kb on - strand, within Pf1N1B4_4051at 4502.674 kb on - strand, within Pf1N1B4_4051at 4502.678 kb on - strand, within Pf1N1B4_4051at 4503.004 kb on - strand, within Pf1N1B4_4051at 4503.020 kb on + strand, within Pf1N1B4_4051at 4503.020 kb on + strand, within Pf1N1B4_4051

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
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4,501,709 - -2.3
4,501,709 - Pf1N1B4_4049 0.13 -1.7
4,501,806 + Pf1N1B4_4049 0.35 +0.0
4,501,854 + Pf1N1B4_4049 0.46 -2.3
4,501,854 + Pf1N1B4_4049 0.46 +0.0
4,501,855 - Pf1N1B4_4049 0.46 -2.5
4,501,881 + Pf1N1B4_4049 0.52 +0.0
4,501,881 + Pf1N1B4_4049 0.52 +0.0
4,501,881 + Pf1N1B4_4049 0.52 -3.3
4,501,887 + -3.8
4,501,887 + Pf1N1B4_4049 0.53 +0.0
4,501,887 + Pf1N1B4_4049 0.53 -1.0
4,501,887 + Pf1N1B4_4049 0.53 +0.0
4,501,887 + Pf1N1B4_4049 0.53 -3.2
4,502,239 + -2.9
4,502,239 + -1.7
4,502,240 - -1.8
4,502,240 - -2.4
4,502,240 - -1.0
4,502,240 - -1.2
4,502,240 - -2.3
4,502,240 - +0.0
4,502,240 - +0.0
4,502,240 - +0.0
4,502,240 - -1.7
4,502,240 - +0.0
4,502,240 - +0.0
4,502,243 + +0.0
4,502,243 + +0.0
4,502,243 + -1.0
4,502,243 + -4.3
4,502,243 + +0.0
4,502,243 + +0.0
4,502,243 + +0.0
4,502,243 + +0.0
4,502,243 + +0.0
4,502,243 + +0.0
4,502,243 + +0.0
4,502,243 + +0.0
4,502,243 + -1.7
4,502,243 + +0.0
4,502,244 - +0.0
4,502,244 - -1.7
4,502,244 - +0.0
4,502,244 - +0.0
4,502,244 - -2.2
4,502,247 + +0.0
4,502,247 + +0.0
4,502,247 + -3.1
4,502,247 + +0.0
4,502,248 - -0.3
4,502,248 - -1.8
4,502,302 + +0.0
4,502,310 + -1.8
4,502,551 - +0.0
4,502,663 - +0.0
4,502,673 + Pf1N1B4_4051 0.10 -1.6
4,502,674 - Pf1N1B4_4051 0.10 -2.0
4,502,674 - Pf1N1B4_4051 0.10 +0.0
4,502,678 - Pf1N1B4_4051 0.10 +0.0
4,503,004 - Pf1N1B4_4051 0.26 +0.0
4,503,020 + Pf1N1B4_4051 0.27 +0.0
4,503,020 + Pf1N1B4_4051 0.27 +0.0

Or see this region's nucleotide sequence