Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4043

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4042 and Pf1N1B4_4043 are separated by 498 nucleotidesPf1N1B4_4043 and Pf1N1B4_4044 are separated by 236 nucleotidesPf1N1B4_4044 and Pf1N1B4_4045 overlap by 8 nucleotides Pf1N1B4_4042 - Isocitrate lyase (EC 4.1.3.1) (from data), at 4,493,842 to 4,495,167 _4042 Pf1N1B4_4043 - Type II and III secretion system family protein, at 4,495,666 to 4,496,427 _4043 Pf1N1B4_4044 - GNAT family acetyltransferase YjcF, at 4,496,664 to 4,497,089 _4044 Pf1N1B4_4045 - FIG002776: hypothetical protein, at 4,497,082 to 4,498,251 _4045 Position (kb) 4495 4496 4497Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 4494.705 kb on - strand, within Pf1N1B4_4042at 4494.705 kb on - strand, within Pf1N1B4_4042at 4494.859 kb on + strand, within Pf1N1B4_4042at 4494.860 kb on - strand, within Pf1N1B4_4042at 4494.860 kb on - strand, within Pf1N1B4_4042at 4494.878 kb on + strand, within Pf1N1B4_4042at 4494.878 kb on + strand, within Pf1N1B4_4042at 4494.879 kb on - strand, within Pf1N1B4_4042at 4495.083 kb on - strandat 4495.187 kb on + strandat 4495.188 kb on - strandat 4495.188 kb on - strandat 4495.267 kb on + strandat 4495.267 kb on + strandat 4495.267 kb on + strandat 4495.268 kb on - strandat 4495.268 kb on - strandat 4495.268 kb on - strandat 4495.268 kb on - strandat 4495.337 kb on + strandat 4495.409 kb on + strandat 4495.409 kb on + strandat 4495.410 kb on - strandat 4495.414 kb on - strandat 4495.623 kb on - strandat 4495.635 kb on + strandat 4495.636 kb on - strandat 4495.638 kb on + strandat 4495.638 kb on + strandat 4495.638 kb on + strandat 4495.668 kb on - strandat 4495.678 kb on - strandat 4495.724 kb on + strandat 4495.724 kb on + strandat 4495.724 kb on + strandat 4495.725 kb on - strandat 4495.837 kb on + strand, within Pf1N1B4_4043at 4495.837 kb on + strandat 4495.838 kb on - strand, within Pf1N1B4_4043at 4495.875 kb on + strand, within Pf1N1B4_4043at 4495.922 kb on + strand, within Pf1N1B4_4043at 4495.974 kb on - strand, within Pf1N1B4_4043at 4496.154 kb on + strand, within Pf1N1B4_4043at 4496.155 kb on - strand, within Pf1N1B4_4043at 4496.428 kb on - strandat 4496.657 kb on - strandat 4496.750 kb on - strand, within Pf1N1B4_4044at 4496.750 kb on - strand, within Pf1N1B4_4044at 4496.750 kb on - strand, within Pf1N1B4_4044at 4497.084 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
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4,494,705 - Pf1N1B4_4042 0.65 +0.0
4,494,705 - Pf1N1B4_4042 0.65 -2.7
4,494,859 + Pf1N1B4_4042 0.77 +0.0
4,494,860 - Pf1N1B4_4042 0.77 -1.4
4,494,860 - Pf1N1B4_4042 0.77 +0.0
4,494,878 + Pf1N1B4_4042 0.78 +0.0
4,494,878 + Pf1N1B4_4042 0.78 +0.0
4,494,879 - Pf1N1B4_4042 0.78 +0.0
4,495,083 - +0.0
4,495,187 + +0.0
4,495,188 - +0.0
4,495,188 - -1.7
4,495,267 + -2.1
4,495,267 + +0.0
4,495,267 + +0.0
4,495,268 - +0.0
4,495,268 - -2.1
4,495,268 - -1.3
4,495,268 - +0.0
4,495,337 + +0.0
4,495,409 + +0.0
4,495,409 + -3.2
4,495,410 - +0.0
4,495,414 - +0.0
4,495,623 - -1.4
4,495,635 + -0.5
4,495,636 - +0.0
4,495,638 + +0.0
4,495,638 + -4.0
4,495,638 + -1.9
4,495,668 - +0.0
4,495,678 - -1.3
4,495,724 + +0.0
4,495,724 + -0.8
4,495,724 + +0.0
4,495,725 - -1.5
4,495,837 + Pf1N1B4_4043 0.22 +0.0
4,495,837 + +0.0
4,495,838 - Pf1N1B4_4043 0.23 -2.3
4,495,875 + Pf1N1B4_4043 0.27 -5.4
4,495,922 + Pf1N1B4_4043 0.34 -1.3
4,495,974 - Pf1N1B4_4043 0.40 +0.0
4,496,154 + Pf1N1B4_4043 0.64 -1.7
4,496,155 - Pf1N1B4_4043 0.64 -3.4
4,496,428 - +0.0
4,496,657 - +0.0
4,496,750 - Pf1N1B4_4044 0.20 -1.2
4,496,750 - Pf1N1B4_4044 0.20 -1.5
4,496,750 - Pf1N1B4_4044 0.20 +0.0
4,497,084 - -2.2

Or see this region's nucleotide sequence