Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4026

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4024 and Pf1N1B4_4025 are separated by 207 nucleotidesPf1N1B4_4025 and Pf1N1B4_4026 overlap by 4 nucleotidesPf1N1B4_4026 and Pf1N1B4_4027 overlap by 2 nucleotidesPf1N1B4_4027 and Pf1N1B4_4028 are separated by 200 nucleotides Pf1N1B4_4024 - Tryptophan synthase beta chain like (EC 4.2.1.20), at 4,476,045 to 4,476,425 _4024 Pf1N1B4_4025 - Outer membrane porin, OprD family, at 4,476,633 to 4,477,394 _4025 Pf1N1B4_4026 - Outer membrane porin, OprD family, at 4,477,391 to 4,477,894 _4026 Pf1N1B4_4027 - hypothetical protein, at 4,477,893 to 4,478,051 _4027 Pf1N1B4_4028 - NADH-ubiquinone oxidoreductase chain N (EC 1.6.5.3), at 4,478,252 to 4,479,715 _4028 Position (kb) 4477 4478Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 4476.423 kb on + strandat 4476.423 kb on + strandat 4476.423 kb on + strandat 4476.424 kb on - strandat 4476.543 kb on + strandat 4476.544 kb on - strandat 4476.584 kb on - strandat 4476.584 kb on - strandat 4476.606 kb on - strandat 4476.653 kb on - strandat 4476.669 kb on + strandat 4476.763 kb on + strandat 4476.764 kb on - strand, within Pf1N1B4_4025at 4476.764 kb on - strand, within Pf1N1B4_4025at 4476.764 kb on - strand, within Pf1N1B4_4025at 4476.764 kb on - strand, within Pf1N1B4_4025at 4476.764 kb on - strand, within Pf1N1B4_4025at 4476.764 kb on - strand, within Pf1N1B4_4025at 4476.835 kb on + strand, within Pf1N1B4_4025at 4476.835 kb on + strand, within Pf1N1B4_4025at 4476.835 kb on + strandat 4476.836 kb on - strand, within Pf1N1B4_4025at 4476.836 kb on - strand, within Pf1N1B4_4025at 4476.836 kb on - strand, within Pf1N1B4_4025at 4476.836 kb on - strand, within Pf1N1B4_4025at 4476.836 kb on - strandat 4476.836 kb on - strand, within Pf1N1B4_4025at 4476.836 kb on - strand, within Pf1N1B4_4025at 4476.836 kb on - strand, within Pf1N1B4_4025at 4476.836 kb on - strand, within Pf1N1B4_4025at 4476.836 kb on - strand, within Pf1N1B4_4025at 4476.836 kb on - strand, within Pf1N1B4_4025at 4476.836 kb on - strand, within Pf1N1B4_4025at 4476.857 kb on - strand, within Pf1N1B4_4025at 4476.913 kb on + strand, within Pf1N1B4_4025at 4476.913 kb on + strand, within Pf1N1B4_4025at 4476.913 kb on + strand, within Pf1N1B4_4025at 4476.914 kb on - strand, within Pf1N1B4_4025at 4476.979 kb on + strand, within Pf1N1B4_4025at 4476.980 kb on - strand, within Pf1N1B4_4025at 4477.162 kb on + strand, within Pf1N1B4_4025at 4477.163 kb on - strand, within Pf1N1B4_4025at 4477.163 kb on - strand, within Pf1N1B4_4025at 4477.163 kb on - strand, within Pf1N1B4_4025at 4477.163 kb on - strand, within Pf1N1B4_4025at 4477.163 kb on - strand, within Pf1N1B4_4025at 4477.163 kb on - strand, within Pf1N1B4_4025at 4477.163 kb on - strand, within Pf1N1B4_4025at 4477.163 kb on - strand, within Pf1N1B4_4025at 4477.163 kb on - strand, within Pf1N1B4_4025at 4477.163 kb on - strand, within Pf1N1B4_4025at 4477.163 kb on - strand, within Pf1N1B4_4025at 4477.166 kb on - strand, within Pf1N1B4_4025at 4477.166 kb on - strand, within Pf1N1B4_4025at 4477.166 kb on - strand, within Pf1N1B4_4025at 4477.166 kb on - strand, within Pf1N1B4_4025at 4477.228 kb on + strand, within Pf1N1B4_4025at 4477.228 kb on + strand, within Pf1N1B4_4025at 4477.229 kb on - strand, within Pf1N1B4_4025at 4477.229 kb on - strand, within Pf1N1B4_4025at 4477.229 kb on - strand, within Pf1N1B4_4025at 4477.232 kb on - strand, within Pf1N1B4_4025at 4477.232 kb on - strand, within Pf1N1B4_4025at 4477.232 kb on - strand, within Pf1N1B4_4025at 4477.265 kb on - strand, within Pf1N1B4_4025at 4477.298 kb on - strand, within Pf1N1B4_4025at 4477.345 kb on + strandat 4477.346 kb on - strandat 4477.541 kb on - strand, within Pf1N1B4_4026at 4477.581 kb on + strand, within Pf1N1B4_4026at 4477.582 kb on - strand, within Pf1N1B4_4026at 4477.582 kb on - strand, within Pf1N1B4_4026at 4477.582 kb on - strand, within Pf1N1B4_4026at 4477.821 kb on + strand, within Pf1N1B4_4026at 4477.822 kb on - strand, within Pf1N1B4_4026at 4477.823 kb on + strandat 4477.823 kb on + strand, within Pf1N1B4_4026at 4477.823 kb on + strand, within Pf1N1B4_4026at 4477.823 kb on + strand, within Pf1N1B4_4026at 4477.823 kb on + strand, within Pf1N1B4_4026at 4477.824 kb on - strand, within Pf1N1B4_4026at 4477.824 kb on - strand, within Pf1N1B4_4026at 4477.824 kb on - strand, within Pf1N1B4_4026at 4477.876 kb on + strandat 4477.946 kb on - strand, within Pf1N1B4_4027at 4477.946 kb on - strand, within Pf1N1B4_4027at 4477.951 kb on + strand, within Pf1N1B4_4027at 4478.095 kb on - strandat 4478.134 kb on + strandat 4478.134 kb on + strandat 4478.134 kb on + strandat 4478.134 kb on + strandat 4478.134 kb on + strandat 4478.134 kb on + strandat 4478.134 kb on + strandat 4478.134 kb on + strandat 4478.134 kb on + strandat 4478.134 kb on + strandat 4478.134 kb on + strandat 4478.134 kb on + strandat 4478.135 kb on - strandat 4478.135 kb on - strandat 4478.135 kb on - strandat 4478.135 kb on - strandat 4478.135 kb on - strandat 4478.135 kb on - strandat 4478.135 kb on - strandat 4478.135 kb on - strandat 4478.135 kb on - strandat 4478.135 kb on - strandat 4478.135 kb on - strandat 4478.135 kb on - strandat 4478.135 kb on - strandat 4478.135 kb on - strandat 4478.135 kb on - strandat 4478.135 kb on - strandat 4478.149 kb on + strandat 4478.150 kb on - strandat 4478.150 kb on - strandat 4478.150 kb on - strandat 4478.150 kb on - strandat 4478.150 kb on - strandat 4478.150 kb on - strandat 4478.150 kb on - strandat 4478.225 kb on - strandat 4478.413 kb on - strand, within Pf1N1B4_4028at 4478.413 kb on - strand, within Pf1N1B4_4028at 4478.413 kb on - strand, within Pf1N1B4_4028at 4478.413 kb on - strand, within Pf1N1B4_4028at 4478.712 kb on + strand, within Pf1N1B4_4028at 4478.713 kb on - strand, within Pf1N1B4_4028

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
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4,476,423 + +0.0
4,476,423 + +0.0
4,476,423 + +0.0
4,476,424 - -3.3
4,476,543 + -2.9
4,476,544 - +1.0
4,476,584 - +0.0
4,476,584 - -2.0
4,476,606 - +0.0
4,476,653 - +0.0
4,476,669 + -4.5
4,476,763 + +0.0
4,476,764 - Pf1N1B4_4025 0.17 +0.0
4,476,764 - Pf1N1B4_4025 0.17 -0.2
4,476,764 - Pf1N1B4_4025 0.17 -3.5
4,476,764 - Pf1N1B4_4025 0.17 +0.0
4,476,764 - Pf1N1B4_4025 0.17 +0.0
4,476,764 - Pf1N1B4_4025 0.17 -1.8
4,476,835 + Pf1N1B4_4025 0.27 -0.3
4,476,835 + Pf1N1B4_4025 0.27 +0.0
4,476,835 + -2.3
4,476,836 - Pf1N1B4_4025 0.27 +0.0
4,476,836 - Pf1N1B4_4025 0.27 -1.7
4,476,836 - Pf1N1B4_4025 0.27 +0.0
4,476,836 - Pf1N1B4_4025 0.27 -1.6
4,476,836 - +0.0
4,476,836 - Pf1N1B4_4025 0.27 -2.1
4,476,836 - Pf1N1B4_4025 0.27 +0.0
4,476,836 - Pf1N1B4_4025 0.27 +0.0
4,476,836 - Pf1N1B4_4025 0.27 -2.1
4,476,836 - Pf1N1B4_4025 0.27 -0.4
4,476,836 - Pf1N1B4_4025 0.27 +0.0
4,476,836 - Pf1N1B4_4025 0.27 +0.0
4,476,857 - Pf1N1B4_4025 0.29 +0.0
4,476,913 + Pf1N1B4_4025 0.37 +0.0
4,476,913 + Pf1N1B4_4025 0.37 +0.0
4,476,913 + Pf1N1B4_4025 0.37 -2.0
4,476,914 - Pf1N1B4_4025 0.37 +0.0
4,476,979 + Pf1N1B4_4025 0.45 -2.7
4,476,980 - Pf1N1B4_4025 0.46 -1.3
4,477,162 + Pf1N1B4_4025 0.69 -2.9
4,477,163 - Pf1N1B4_4025 0.70 -1.2
4,477,163 - Pf1N1B4_4025 0.70 -1.6
4,477,163 - Pf1N1B4_4025 0.70 -1.6
4,477,163 - Pf1N1B4_4025 0.70 -2.0
4,477,163 - Pf1N1B4_4025 0.70 +0.0
4,477,163 - Pf1N1B4_4025 0.70 +0.0
4,477,163 - Pf1N1B4_4025 0.70 -2.3
4,477,163 - Pf1N1B4_4025 0.70 +0.0
4,477,163 - Pf1N1B4_4025 0.70 +0.0
4,477,163 - Pf1N1B4_4025 0.70 -1.3
4,477,163 - Pf1N1B4_4025 0.70 +0.0
4,477,166 - Pf1N1B4_4025 0.70 +0.0
4,477,166 - Pf1N1B4_4025 0.70 +0.0
4,477,166 - Pf1N1B4_4025 0.70 -1.0
4,477,166 - Pf1N1B4_4025 0.70 -1.7
4,477,228 + Pf1N1B4_4025 0.78 +0.0
4,477,228 + Pf1N1B4_4025 0.78 -0.3
4,477,229 - Pf1N1B4_4025 0.78 -1.5
4,477,229 - Pf1N1B4_4025 0.78 -0.8
4,477,229 - Pf1N1B4_4025 0.78 +0.0
4,477,232 - Pf1N1B4_4025 0.79 +0.0
4,477,232 - Pf1N1B4_4025 0.79 +0.0
4,477,232 - Pf1N1B4_4025 0.79 -1.6
4,477,265 - Pf1N1B4_4025 0.83 +0.0
4,477,298 - Pf1N1B4_4025 0.87 -2.4
4,477,345 + +0.0
4,477,346 - +0.0
4,477,541 - Pf1N1B4_4026 0.30 -2.9
4,477,581 + Pf1N1B4_4026 0.38 +0.0
4,477,582 - Pf1N1B4_4026 0.38 -1.6
4,477,582 - Pf1N1B4_4026 0.38 +0.0
4,477,582 - Pf1N1B4_4026 0.38 -2.5
4,477,821 + Pf1N1B4_4026 0.85 +0.0
4,477,822 - Pf1N1B4_4026 0.86 +0.0
4,477,823 + +1.1
4,477,823 + Pf1N1B4_4026 0.86 -2.4
4,477,823 + Pf1N1B4_4026 0.86 +0.0
4,477,823 + Pf1N1B4_4026 0.86 +0.0
4,477,823 + Pf1N1B4_4026 0.86 +0.0
4,477,824 - Pf1N1B4_4026 0.86 +0.0
4,477,824 - Pf1N1B4_4026 0.86 +0.0
4,477,824 - Pf1N1B4_4026 0.86 -2.5
4,477,876 + -1.1
4,477,946 - Pf1N1B4_4027 0.33 -0.8
4,477,946 - Pf1N1B4_4027 0.33 +0.0
4,477,951 + Pf1N1B4_4027 0.36 -2.0
4,478,095 - -1.7
4,478,134 + +0.0
4,478,134 + -2.4
4,478,134 + -2.4
4,478,134 + +0.0
4,478,134 + -2.0
4,478,134 + +0.0
4,478,134 + +0.0
4,478,134 + -1.8
4,478,134 + +0.0
4,478,134 + -1.8
4,478,134 + +0.0
4,478,134 + -1.8
4,478,135 - +0.0
4,478,135 - +1.6
4,478,135 - +0.0
4,478,135 - -1.4
4,478,135 - +0.0
4,478,135 - -2.3
4,478,135 - -2.3
4,478,135 - +0.0
4,478,135 - -2.1
4,478,135 - +0.0
4,478,135 - -1.4
4,478,135 - +0.0
4,478,135 - -4.5
4,478,135 - +0.0
4,478,135 - +0.0
4,478,135 - +0.0
4,478,149 + -2.2
4,478,150 - +0.0
4,478,150 - -1.0
4,478,150 - -1.0
4,478,150 - -0.5
4,478,150 - -2.6
4,478,150 - +0.0
4,478,150 - +0.0
4,478,225 - +0.0
4,478,413 - Pf1N1B4_4028 0.11 +0.0
4,478,413 - Pf1N1B4_4028 0.11 -2.7
4,478,413 - Pf1N1B4_4028 0.11 +0.0
4,478,413 - Pf1N1B4_4028 0.11 +0.0
4,478,712 + Pf1N1B4_4028 0.31 -1.5
4,478,713 - Pf1N1B4_4028 0.31 +0.0

Or see this region's nucleotide sequence