Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_381

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_380 and Pf1N1B4_381 are separated by 293 nucleotidesPf1N1B4_381 and Pf1N1B4_382 are separated by 60 nucleotides Pf1N1B4_380 - Oxidoreductase, short chain dehydrogenase/reductase family, at 449,774 to 450,589 _380 Pf1N1B4_381 - hypothetical protein, at 450,883 to 451,035 _381 Pf1N1B4_382 - Phosphoglycerol transferase and related proteins, alkaline phosphatase superfamily, at 451,096 to 453,189 _382 Position (kb) 450 451 452Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 449.928 kb on + strand, within Pf1N1B4_380at 449.934 kb on + strand, within Pf1N1B4_380at 449.934 kb on + strand, within Pf1N1B4_380at 449.934 kb on + strand, within Pf1N1B4_380at 449.935 kb on - strand, within Pf1N1B4_380at 449.935 kb on - strand, within Pf1N1B4_380at 449.935 kb on - strandat 450.141 kb on + strand, within Pf1N1B4_380at 450.141 kb on + strand, within Pf1N1B4_380at 450.141 kb on + strand, within Pf1N1B4_380at 450.141 kb on + strand, within Pf1N1B4_380at 450.142 kb on - strand, within Pf1N1B4_380at 450.142 kb on - strand, within Pf1N1B4_380at 450.142 kb on - strandat 450.142 kb on - strand, within Pf1N1B4_380at 450.142 kb on - strand, within Pf1N1B4_380at 450.142 kb on - strand, within Pf1N1B4_380at 450.641 kb on - strandat 450.727 kb on - strandat 450.735 kb on + strandat 450.752 kb on + strandat 450.753 kb on - strandat 450.757 kb on + strandat 450.803 kb on - strandat 450.810 kb on + strandat 450.810 kb on + strandat 450.816 kb on + strandat 450.817 kb on - strandat 450.819 kb on + strandat 450.819 kb on + strandat 450.819 kb on + strandat 450.819 kb on + strandat 450.819 kb on + strandat 450.819 kb on + strandat 450.820 kb on - strandat 450.820 kb on - strandat 450.820 kb on - strandat 450.846 kb on + strandat 450.897 kb on + strandat 450.910 kb on - strandat 450.923 kb on + strand, within Pf1N1B4_381at 450.923 kb on + strand, within Pf1N1B4_381at 450.923 kb on + strand, within Pf1N1B4_381at 450.923 kb on + strand, within Pf1N1B4_381at 450.923 kb on + strand, within Pf1N1B4_381at 450.924 kb on - strand, within Pf1N1B4_381at 451.004 kb on + strand, within Pf1N1B4_381at 451.020 kb on - strandat 451.031 kb on + strandat 451.032 kb on - strandat 451.032 kb on - strandat 451.033 kb on + strandat 451.033 kb on + strandat 451.033 kb on + strandat 451.083 kb on - strandat 451.250 kb on + strandat 451.250 kb on + strandat 451.251 kb on - strandat 451.251 kb on - strandat 451.251 kb on - strandat 451.461 kb on + strand, within Pf1N1B4_382at 451.462 kb on - strand, within Pf1N1B4_382at 451.608 kb on - strand, within Pf1N1B4_382at 451.608 kb on - strand, within Pf1N1B4_382at 451.608 kb on - strand, within Pf1N1B4_382at 451.608 kb on - strand, within Pf1N1B4_382at 451.772 kb on + strand, within Pf1N1B4_382at 451.772 kb on + strand, within Pf1N1B4_382at 451.772 kb on + strand, within Pf1N1B4_382at 451.772 kb on + strand, within Pf1N1B4_382at 451.773 kb on - strand, within Pf1N1B4_382at 451.773 kb on - strand, within Pf1N1B4_382at 451.890 kb on + strand, within Pf1N1B4_382at 451.890 kb on + strand, within Pf1N1B4_382at 451.891 kb on - strand, within Pf1N1B4_382at 451.891 kb on - strandat 451.964 kb on + strand, within Pf1N1B4_382at 451.964 kb on + strand, within Pf1N1B4_382at 451.964 kb on + strand, within Pf1N1B4_382at 451.965 kb on - strand, within Pf1N1B4_382at 451.965 kb on - strand, within Pf1N1B4_382at 451.965 kb on - strand, within Pf1N1B4_382at 451.965 kb on - strand, within Pf1N1B4_382at 451.965 kb on - strand, within Pf1N1B4_382at 451.970 kb on + strand, within Pf1N1B4_382at 451.970 kb on + strandat 451.970 kb on + strand, within Pf1N1B4_382at 451.970 kb on + strand, within Pf1N1B4_382at 451.970 kb on + strand, within Pf1N1B4_382at 451.970 kb on + strand, within Pf1N1B4_382at 451.970 kb on + strand, within Pf1N1B4_382at 451.970 kb on - strand, within Pf1N1B4_382at 451.971 kb on - strand, within Pf1N1B4_382at 451.971 kb on - strand, within Pf1N1B4_382at 451.971 kb on - strand, within Pf1N1B4_382at 451.971 kb on - strand, within Pf1N1B4_382

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
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449,928 + Pf1N1B4_380 0.19 -0.0
449,934 + Pf1N1B4_380 0.20 -1.5
449,934 + Pf1N1B4_380 0.20 -1.2
449,934 + Pf1N1B4_380 0.20 -0.0
449,935 - Pf1N1B4_380 0.20 -1.1
449,935 - Pf1N1B4_380 0.20 -0.0
449,935 - -1.2
450,141 + Pf1N1B4_380 0.45 -0.0
450,141 + Pf1N1B4_380 0.45 -0.0
450,141 + Pf1N1B4_380 0.45 -1.4
450,141 + Pf1N1B4_380 0.45 -0.0
450,142 - Pf1N1B4_380 0.45 -0.0
450,142 - Pf1N1B4_380 0.45 -0.0
450,142 - -1.2
450,142 - Pf1N1B4_380 0.45 -6.0
450,142 - Pf1N1B4_380 0.45 -0.0
450,142 - Pf1N1B4_380 0.45 -1.5
450,641 - -0.0
450,727 - -0.0
450,735 + -2.9
450,752 + -2.1
450,753 - -0.0
450,757 + -1.1
450,803 - -0.0
450,810 + -1.7
450,810 + -3.3
450,816 + -0.0
450,817 - -0.0
450,819 + -0.0
450,819 + -1.5
450,819 + -1.4
450,819 + -1.6
450,819 + -2.4
450,819 + -1.9
450,820 - -2.3
450,820 - -0.0
450,820 - -0.6
450,846 + -2.2
450,897 + -0.0
450,910 - -1.0
450,923 + Pf1N1B4_381 0.26 -0.0
450,923 + Pf1N1B4_381 0.26 -0.0
450,923 + Pf1N1B4_381 0.26 -4.6
450,923 + Pf1N1B4_381 0.26 -0.0
450,923 + Pf1N1B4_381 0.26 -0.0
450,924 - Pf1N1B4_381 0.27 -2.0
451,004 + Pf1N1B4_381 0.79 -0.0
451,020 - +1.0
451,031 + -2.2
451,032 - -1.6
451,032 - -0.0
451,033 + -1.8
451,033 + -0.0
451,033 + -0.0
451,083 - -1.5
451,250 + -0.0
451,250 + -0.0
451,251 - -0.0
451,251 - -0.0
451,251 - -3.5
451,461 + Pf1N1B4_382 0.17 -0.0
451,462 - Pf1N1B4_382 0.17 -0.0
451,608 - Pf1N1B4_382 0.24 -0.0
451,608 - Pf1N1B4_382 0.24 -0.0
451,608 - Pf1N1B4_382 0.24 -1.5
451,608 - Pf1N1B4_382 0.24 -2.1
451,772 + Pf1N1B4_382 0.32 -0.0
451,772 + Pf1N1B4_382 0.32 -2.4
451,772 + Pf1N1B4_382 0.32 -3.0
451,772 + Pf1N1B4_382 0.32 -0.0
451,773 - Pf1N1B4_382 0.32 -0.0
451,773 - Pf1N1B4_382 0.32 -2.3
451,890 + Pf1N1B4_382 0.38 -0.0
451,890 + Pf1N1B4_382 0.38 -0.0
451,891 - Pf1N1B4_382 0.38 -0.0
451,891 - -3.2
451,964 + Pf1N1B4_382 0.41 -1.8
451,964 + Pf1N1B4_382 0.41 -1.0
451,964 + Pf1N1B4_382 0.41 -2.0
451,965 - Pf1N1B4_382 0.41 -2.2
451,965 - Pf1N1B4_382 0.41 -5.8
451,965 - Pf1N1B4_382 0.41 -0.0
451,965 - Pf1N1B4_382 0.41 -0.0
451,965 - Pf1N1B4_382 0.41 -0.0
451,970 + Pf1N1B4_382 0.42 -0.0
451,970 + +1.0
451,970 + Pf1N1B4_382 0.42 -1.5
451,970 + Pf1N1B4_382 0.42 -0.0
451,970 + Pf1N1B4_382 0.42 -0.0
451,970 + Pf1N1B4_382 0.42 -1.6
451,970 + Pf1N1B4_382 0.42 -0.0
451,970 - Pf1N1B4_382 0.42 -0.0
451,971 - Pf1N1B4_382 0.42 -1.0
451,971 - Pf1N1B4_382 0.42 -0.0
451,971 - Pf1N1B4_382 0.42 -0.0
451,971 - Pf1N1B4_382 0.42 -2.5

Or see this region's nucleotide sequence