Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_372 and Pf1N1B4_373 are separated by 323 nucleotides Pf1N1B4_373 and Pf1N1B4_374 are separated by 123 nucleotides
Pf1N1B4_372 - UDP-galactose-lipid carrier transferase (EC 2.-.-.-), at 439,678 to 441,201
_372
Pf1N1B4_373 - 5-methylaminomethyl-2-thiouridine-forming enzyme mnmC, at 441,525 to 443,507
_373
Pf1N1B4_374 - Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4), at 443,631 to 445,403
_374
Position (kb) 441
442
443
444 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 440.547 kb on + strand, within Pf1N1B4_372 at 440.549 kb on + strand, within Pf1N1B4_372 at 440.550 kb on - strand, within Pf1N1B4_372 at 440.624 kb on + strand, within Pf1N1B4_372 at 440.624 kb on + strand at 440.625 kb on - strand, within Pf1N1B4_372 at 440.643 kb on - strand, within Pf1N1B4_372 at 441.155 kb on + strand at 441.155 kb on + strand at 441.155 kb on + strand at 441.156 kb on - strand at 441.156 kb on - strand at 441.156 kb on - strand at 441.201 kb on + strand at 441.201 kb on + strand at 441.202 kb on - strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.251 kb on - strand at 441.251 kb on - strand at 441.251 kb on - strand at 441.251 kb on - strand at 441.251 kb on - strand at 441.254 kb on + strand at 441.254 kb on + strand at 441.254 kb on + strand at 441.255 kb on - strand at 441.255 kb on - strand at 441.255 kb on - strand at 441.255 kb on - strand at 441.360 kb on + strand at 441.360 kb on + strand at 441.361 kb on - strand at 441.361 kb on - strand at 441.361 kb on - strand at 441.361 kb on - strand at 441.364 kb on + strand at 441.364 kb on + strand at 441.364 kb on + strand at 441.364 kb on + strand at 441.364 kb on + strand at 441.364 kb on + strand at 441.365 kb on - strand at 441.365 kb on - strand at 441.365 kb on - strand at 441.365 kb on - strand at 441.365 kb on - strand at 441.365 kb on - strand at 441.603 kb on + strand at 441.603 kb on + strand at 441.603 kb on + strand at 441.603 kb on + strand at 441.603 kb on + strand at 441.900 kb on + strand, within Pf1N1B4_373 at 442.203 kb on - strand, within Pf1N1B4_373 at 442.449 kb on + strand at 442.449 kb on + strand, within Pf1N1B4_373 at 442.711 kb on - strand, within Pf1N1B4_373 at 443.211 kb on - strand, within Pf1N1B4_373 at 443.211 kb on - strand, within Pf1N1B4_373 at 443.275 kb on - strand, within Pf1N1B4_373 at 443.518 kb on + strand at 443.518 kb on + strand at 443.518 kb on + strand at 443.519 kb on - strand at 443.519 kb on - strand at 443.520 kb on + strand at 443.520 kb on + strand at 443.520 kb on + strand at 443.521 kb on - strand at 443.590 kb on + strand at 443.630 kb on + strand at 443.630 kb on + strand at 443.630 kb on + strand at 443.630 kb on + strand at 443.630 kb on + strand at 443.631 kb on - strand at 443.631 kb on - strand at 443.631 kb on - strand at 443.631 kb on - strand at 443.631 kb on - strand at 443.631 kb on - strand at 443.631 kb on - strand at 443.641 kb on + strand at 443.654 kb on - strand at 443.654 kb on - strand at 443.865 kb on + strand, within Pf1N1B4_374 at 443.866 kb on - strand, within Pf1N1B4_374 at 443.866 kb on - strand, within Pf1N1B4_374 at 443.916 kb on + strand at 443.917 kb on - strand, within Pf1N1B4_374 at 443.917 kb on - strand, within Pf1N1B4_374 at 443.953 kb on - strand, within Pf1N1B4_374 at 444.072 kb on + strand, within Pf1N1B4_374 at 444.072 kb on + strand, within Pf1N1B4_374 at 444.072 kb on + strand, within Pf1N1B4_374 at 444.073 kb on - strand, within Pf1N1B4_374 at 444.073 kb on - strand, within Pf1N1B4_374 at 444.073 kb on - strand, within Pf1N1B4_374 at 444.177 kb on + strand, within Pf1N1B4_374 at 444.460 kb on + strand, within Pf1N1B4_374 at 444.460 kb on + strand, within Pf1N1B4_374
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 440,547 + Pf1N1B4_372 0.57 -0.0 440,549 + Pf1N1B4_372 0.57 -0.0 440,550 - Pf1N1B4_372 0.57 -1.3 440,624 + Pf1N1B4_372 0.62 -0.0 440,624 + -0.9 440,625 - Pf1N1B4_372 0.62 -0.0 440,643 - Pf1N1B4_372 0.63 -0.0 441,155 + -1.9 441,155 + -0.0 441,155 + -0.0 441,156 - -0.0 441,156 - -0.0 441,156 - -0.0 441,201 + -0.6 441,201 + -1.5 441,202 - -0.6 441,250 + -0.0 441,250 + -0.8 441,250 + -0.5 441,250 + -0.0 441,250 + +2.6 441,250 + -0.0 441,250 + -0.0 441,250 + -0.0 441,250 + -0.0 441,251 - -0.0 441,251 - -0.0 441,251 - -0.0 441,251 - -0.0 441,251 - -0.0 441,254 + -0.0 441,254 + -0.0 441,254 + -1.9 441,255 - -0.0 441,255 - -0.0 441,255 - -0.0 441,255 - -3.0 441,360 + -0.0 441,360 + -1.0 441,361 - -3.4 441,361 - -0.0 441,361 - -0.0 441,361 - -2.0 441,364 + -0.0 441,364 + -0.0 441,364 + -0.0 441,364 + -2.3 441,364 + -1.4 441,364 + -0.0 441,365 - -0.0 441,365 - -0.0 441,365 - -0.0 441,365 - -0.0 441,365 - -0.0 441,365 - -2.8 441,603 + -0.0 441,603 + -0.0 441,603 + -2.0 441,603 + -2.1 441,603 + -2.4 441,900 + Pf1N1B4_373 0.19 -0.0 442,203 - Pf1N1B4_373 0.34 -2.0 442,449 + -0.0 442,449 + Pf1N1B4_373 0.47 -0.0 442,711 - Pf1N1B4_373 0.60 -3.8 443,211 - Pf1N1B4_373 0.85 -0.0 443,211 - Pf1N1B4_373 0.85 -0.0 443,275 - Pf1N1B4_373 0.88 -0.0 443,518 + -0.0 443,518 + -0.0 443,518 + -1.7 443,519 - -0.0 443,519 - +1.0 443,520 + -0.0 443,520 + -0.8 443,520 + -0.0 443,521 - -0.0 443,590 + -1.1 443,630 + -0.0 443,630 + -2.9 443,630 + -0.0 443,630 + -0.0 443,630 + -0.0 443,631 - -0.0 443,631 - -0.0 443,631 - -0.0 443,631 - -0.0 443,631 - -2.4 443,631 - -0.0 443,631 - -0.0 443,641 + -0.0 443,654 - -3.8 443,654 - -0.0 443,865 + Pf1N1B4_374 0.13 -2.2 443,866 - Pf1N1B4_374 0.13 -3.8 443,866 - Pf1N1B4_374 0.13 -1.2 443,916 + -0.0 443,917 - Pf1N1B4_374 0.16 -0.0 443,917 - Pf1N1B4_374 0.16 -0.0 443,953 - Pf1N1B4_374 0.18 -0.0 444,072 + Pf1N1B4_374 0.25 -0.0 444,072 + Pf1N1B4_374 0.25 -2.2 444,072 + Pf1N1B4_374 0.25 -2.6 444,073 - Pf1N1B4_374 0.25 -0.0 444,073 - Pf1N1B4_374 0.25 -0.0 444,073 - Pf1N1B4_374 0.25 -2.1 444,177 + Pf1N1B4_374 0.31 -1.7 444,460 + Pf1N1B4_374 0.47 -0.0 444,460 + Pf1N1B4_374 0.47 -0.0
Or see this region's nucleotide sequence