Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3547

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3545 and Pf1N1B4_3546 are separated by 175 nucleotidesPf1N1B4_3546 and Pf1N1B4_3547 are separated by 363 nucleotidesPf1N1B4_3547 and Pf1N1B4_3548 are separated by 7 nucleotidesPf1N1B4_3548 and Pf1N1B4_3549 are separated by 110 nucleotidesPf1N1B4_3549 and Pf1N1B4_3550 are separated by 1 nucleotides Pf1N1B4_3545 - Putative two-component system sensor kinase, at 3,951,256 to 3,952,728 _3545 Pf1N1B4_3546 - P pilus assembly/Cpx signaling pathway, periplasmic inhibitor/zinc-resistance associated protein, at 3,952,904 to 3,953,353 _3546 Pf1N1B4_3547 - DNA-binding response regulator, at 3,953,717 to 3,954,394 _3547 Pf1N1B4_3548 - Translation initiation factor 2 (IF-2; GTPase), at 3,954,402 to 3,954,815 _3548 Pf1N1B4_3549 - YciL protein, at 3,954,926 to 3,955,225 _3549 Pf1N1B4_3550 - Intracellular septation protein IspA, at 3,955,227 to 3,955,823 _3550 Position (kb) 3953 3954 3955Strain fitness (log2 ratio) -3 -2 -1 0 1at 3952.808 kb on - strandat 3952.848 kb on + strandat 3952.848 kb on + strandat 3952.875 kb on + strandat 3952.875 kb on + strandat 3952.876 kb on - strandat 3952.876 kb on - strandat 3952.906 kb on - strandat 3953.112 kb on + strand, within Pf1N1B4_3546at 3953.113 kb on - strand, within Pf1N1B4_3546at 3953.113 kb on - strandat 3953.358 kb on + strandat 3953.358 kb on + strandat 3953.359 kb on - strandat 3953.359 kb on - strandat 3953.359 kb on - strandat 3953.359 kb on - strandat 3953.437 kb on + strandat 3953.437 kb on + strandat 3953.438 kb on - strandat 3953.438 kb on - strandat 3953.438 kb on - strandat 3953.520 kb on + strandat 3953.521 kb on - strandat 3953.521 kb on - strandat 3953.614 kb on - strandat 3953.660 kb on + strandat 3953.701 kb on - strandat 3953.727 kb on + strandat 3953.727 kb on + strandat 3953.728 kb on - strandat 3953.730 kb on + strandat 3953.730 kb on + strandat 3953.730 kb on + strandat 3953.730 kb on + strandat 3953.731 kb on - strandat 3953.731 kb on - strandat 3953.731 kb on - strandat 3953.731 kb on - strandat 3953.731 kb on - strandat 3953.733 kb on + strandat 3953.832 kb on + strand, within Pf1N1B4_3547at 3953.833 kb on - strand, within Pf1N1B4_3547at 3954.100 kb on - strand, within Pf1N1B4_3547at 3954.340 kb on + strandat 3954.340 kb on + strandat 3954.341 kb on - strandat 3954.377 kb on + strandat 3954.377 kb on + strandat 3954.377 kb on + strandat 3954.378 kb on - strandat 3954.378 kb on - strandat 3954.378 kb on - strandat 3954.380 kb on + strandat 3954.381 kb on - strandat 3954.474 kb on + strand, within Pf1N1B4_3548at 3954.622 kb on + strand, within Pf1N1B4_3548at 3954.624 kb on - strand, within Pf1N1B4_3548at 3954.834 kb on + strandat 3954.873 kb on - strandat 3954.987 kb on + strand, within Pf1N1B4_3549at 3954.987 kb on + strand, within Pf1N1B4_3549at 3954.988 kb on - strand, within Pf1N1B4_3549at 3954.988 kb on - strandat 3954.988 kb on - strand, within Pf1N1B4_3549at 3955.112 kb on + strand, within Pf1N1B4_3549at 3955.113 kb on - strand, within Pf1N1B4_3549at 3955.133 kb on - strandat 3955.133 kb on - strand, within Pf1N1B4_3549at 3955.219 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
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3,952,808 - -0.0
3,952,848 + -0.0
3,952,848 + -0.0
3,952,875 + -0.0
3,952,875 + -1.0
3,952,876 - -0.0
3,952,876 - -0.0
3,952,906 - -0.8
3,953,112 + Pf1N1B4_3546 0.46 -1.9
3,953,113 - Pf1N1B4_3546 0.46 -0.0
3,953,113 - -2.1
3,953,358 + -0.2
3,953,358 + -0.0
3,953,359 - -0.0
3,953,359 - -0.0
3,953,359 - -2.2
3,953,359 - -1.6
3,953,437 + -0.0
3,953,437 + -1.7
3,953,438 - -0.0
3,953,438 - -0.9
3,953,438 - -0.0
3,953,520 + -0.0
3,953,521 - -0.0
3,953,521 - -0.0
3,953,614 - -0.0
3,953,660 + -0.0
3,953,701 - -0.0
3,953,727 + -0.0
3,953,727 + -0.0
3,953,728 - -1.7
3,953,730 + -0.0
3,953,730 + -2.7
3,953,730 + -0.0
3,953,730 + -1.4
3,953,731 - -0.0
3,953,731 - +1.0
3,953,731 - -1.8
3,953,731 - -0.0
3,953,731 - -1.3
3,953,733 + -0.0
3,953,832 + Pf1N1B4_3547 0.17 -2.6
3,953,833 - Pf1N1B4_3547 0.17 -0.0
3,954,100 - Pf1N1B4_3547 0.56 -0.0
3,954,340 + -0.0
3,954,340 + +1.0
3,954,341 - -0.0
3,954,377 + -0.0
3,954,377 + -3.0
3,954,377 + -0.0
3,954,378 - -0.0
3,954,378 - -0.0
3,954,378 - -0.0
3,954,380 + -0.0
3,954,381 - -1.2
3,954,474 + Pf1N1B4_3548 0.17 -0.0
3,954,622 + Pf1N1B4_3548 0.53 -0.0
3,954,624 - Pf1N1B4_3548 0.54 -0.0
3,954,834 + -0.0
3,954,873 - -0.0
3,954,987 + Pf1N1B4_3549 0.20 -0.0
3,954,987 + Pf1N1B4_3549 0.20 -0.0
3,954,988 - Pf1N1B4_3549 0.21 -0.0
3,954,988 - -0.0
3,954,988 - Pf1N1B4_3549 0.21 -2.2
3,955,112 + Pf1N1B4_3549 0.62 -1.8
3,955,113 - Pf1N1B4_3549 0.62 -0.0
3,955,133 - -0.0
3,955,133 - Pf1N1B4_3549 0.69 -0.0
3,955,219 - -1.8

Or see this region's nucleotide sequence