Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_349 and Pf1N1B4_350 are separated by 12 nucleotides Pf1N1B4_350 and Pf1N1B4_351 overlap by 1 nucleotides Pf1N1B4_351 and Pf1N1B4_352 are separated by 1 nucleotides
Pf1N1B4_349 - Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57), at 414,116 to 415,228
_349
Pf1N1B4_350 - Chorismate mutase I (EC 5.4.99.5) / Prephenate dehydratase (EC 4.2.1.51), at 415,241 to 416,335
_350
Pf1N1B4_351 - Phosphoserine aminotransferase (EC 2.6.1.52), at 416,335 to 417,213
_351
Pf1N1B4_352 - Phosphoserine aminotransferase (EC 2.6.1.52), at 417,215 to 417,427
_352
Position (kb) 415
416
417 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 414.396 kb on + strand, within Pf1N1B4_349 at 414.396 kb on + strand, within Pf1N1B4_349 at 414.397 kb on - strand, within Pf1N1B4_349 at 414.397 kb on - strand, within Pf1N1B4_349 at 414.535 kb on - strand, within Pf1N1B4_349 at 414.535 kb on - strand, within Pf1N1B4_349 at 414.585 kb on + strand, within Pf1N1B4_349 at 414.586 kb on - strand, within Pf1N1B4_349 at 414.622 kb on - strand, within Pf1N1B4_349 at 414.630 kb on + strand, within Pf1N1B4_349 at 414.631 kb on - strand, within Pf1N1B4_349 at 414.631 kb on - strand, within Pf1N1B4_349 at 414.631 kb on - strand, within Pf1N1B4_349 at 414.631 kb on - strand, within Pf1N1B4_349 at 414.852 kb on + strand, within Pf1N1B4_349 at 414.904 kb on - strand, within Pf1N1B4_349 at 415.174 kb on - strand at 415.174 kb on - strand at 415.284 kb on - strand at 415.284 kb on - strand at 415.284 kb on - strand at 415.311 kb on - strand at 415.365 kb on + strand, within Pf1N1B4_350 at 415.365 kb on + strand, within Pf1N1B4_350 at 415.365 kb on + strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.376 kb on - strand, within Pf1N1B4_350 at 415.376 kb on - strand, within Pf1N1B4_350 at 415.376 kb on - strand, within Pf1N1B4_350 at 415.755 kb on + strand, within Pf1N1B4_350 at 415.756 kb on - strand, within Pf1N1B4_350 at 415.756 kb on - strand at 415.756 kb on - strand, within Pf1N1B4_350 at 415.756 kb on - strand, within Pf1N1B4_350 at 415.756 kb on - strand, within Pf1N1B4_350 at 415.756 kb on - strand, within Pf1N1B4_350 at 415.756 kb on - strand, within Pf1N1B4_350 at 416.043 kb on + strand, within Pf1N1B4_350 at 416.226 kb on - strand at 416.226 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 414,396 + Pf1N1B4_349 0.25 -0.0 414,396 + Pf1N1B4_349 0.25 -1.6 414,397 - Pf1N1B4_349 0.25 -2.6 414,397 - Pf1N1B4_349 0.25 -0.0 414,535 - Pf1N1B4_349 0.38 -0.0 414,535 - Pf1N1B4_349 0.38 -0.0 414,585 + Pf1N1B4_349 0.42 -0.0 414,586 - Pf1N1B4_349 0.42 -2.0 414,622 - Pf1N1B4_349 0.45 -0.0 414,630 + Pf1N1B4_349 0.46 -0.0 414,631 - Pf1N1B4_349 0.46 -0.0 414,631 - Pf1N1B4_349 0.46 -0.0 414,631 - Pf1N1B4_349 0.46 -1.9 414,631 - Pf1N1B4_349 0.46 -0.0 414,852 + Pf1N1B4_349 0.66 -0.0 414,904 - Pf1N1B4_349 0.71 -0.9 415,174 - -0.0 415,174 - -0.0 415,284 - -0.0 415,284 - -0.0 415,284 - -2.0 415,311 - -2.0 415,365 + Pf1N1B4_350 0.11 -3.0 415,365 + Pf1N1B4_350 0.11 -1.0 415,365 + Pf1N1B4_350 0.11 -2.4 415,366 - Pf1N1B4_350 0.11 -3.5 415,366 - Pf1N1B4_350 0.11 -0.0 415,366 - Pf1N1B4_350 0.11 -2.6 415,366 - Pf1N1B4_350 0.11 -0.0 415,366 - Pf1N1B4_350 0.11 -0.0 415,366 - Pf1N1B4_350 0.11 -0.0 415,366 - Pf1N1B4_350 0.11 -3.2 415,366 - Pf1N1B4_350 0.11 -2.0 415,366 - Pf1N1B4_350 0.11 -0.0 415,366 - Pf1N1B4_350 0.11 -1.0 415,366 - Pf1N1B4_350 0.11 -1.0 415,366 - Pf1N1B4_350 0.11 -1.0 415,366 - Pf1N1B4_350 0.11 -0.0 415,366 - Pf1N1B4_350 0.11 -0.0 415,366 - Pf1N1B4_350 0.11 -0.0 415,366 - Pf1N1B4_350 0.11 -0.0 415,366 - Pf1N1B4_350 0.11 -0.0 415,366 - Pf1N1B4_350 0.11 -1.6 415,366 - Pf1N1B4_350 0.11 -0.0 415,376 - Pf1N1B4_350 0.12 -2.4 415,376 - Pf1N1B4_350 0.12 -0.0 415,376 - Pf1N1B4_350 0.12 -0.0 415,755 + Pf1N1B4_350 0.47 -0.0 415,756 - Pf1N1B4_350 0.47 -2.8 415,756 - -0.0 415,756 - Pf1N1B4_350 0.47 -0.0 415,756 - Pf1N1B4_350 0.47 -1.0 415,756 - Pf1N1B4_350 0.47 -3.7 415,756 - Pf1N1B4_350 0.47 +1.0 415,756 - Pf1N1B4_350 0.47 -0.0 416,043 + Pf1N1B4_350 0.73 -0.0 416,226 - +0.7 416,226 - -0.0
Or see this region's nucleotide sequence