Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3370 and Pf1N1B4_3371 are separated by 244 nucleotides Pf1N1B4_3371 and Pf1N1B4_3372 overlap by 13 nucleotides Pf1N1B4_3372 and Pf1N1B4_3373 overlap by 4 nucleotides
Pf1N1B4_3370 - ATPase associated with various cellular activities, AAA_5, at 3,758,058 to 3,758,903
_3370
Pf1N1B4_3371 - FIG00954979: hypothetical protein, at 3,759,148 to 3,759,336
_3371
Pf1N1B4_3372 - FIG00962464: hypothetical protein, at 3,759,324 to 3,759,827
_3372
Pf1N1B4_3373 - FIG00954689: hypothetical protein, at 3,759,824 to 3,760,900
_3373
Position (kb)
3759
3760 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3758.377 kb on + strand, within Pf1N1B4_3370 at 3758.377 kb on + strand, within Pf1N1B4_3370 at 3758.377 kb on + strand at 3758.377 kb on + strand, within Pf1N1B4_3370 at 3758.377 kb on + strand, within Pf1N1B4_3370 at 3758.378 kb on - strand, within Pf1N1B4_3370 at 3758.378 kb on - strand, within Pf1N1B4_3370 at 3758.435 kb on + strand, within Pf1N1B4_3370 at 3758.435 kb on + strand, within Pf1N1B4_3370 at 3758.436 kb on - strand, within Pf1N1B4_3370 at 3758.436 kb on - strand, within Pf1N1B4_3370 at 3758.491 kb on + strand, within Pf1N1B4_3370 at 3758.491 kb on + strand, within Pf1N1B4_3370 at 3758.491 kb on + strand, within Pf1N1B4_3370 at 3758.492 kb on - strand, within Pf1N1B4_3370 at 3758.492 kb on - strand, within Pf1N1B4_3370 at 3758.492 kb on - strand, within Pf1N1B4_3370 at 3758.623 kb on + strand, within Pf1N1B4_3370 at 3758.738 kb on + strand, within Pf1N1B4_3370 at 3758.878 kb on + strand at 3758.879 kb on - strand at 3758.879 kb on - strand at 3758.957 kb on + strand at 3758.957 kb on + strand at 3758.958 kb on - strand at 3758.958 kb on - strand at 3758.958 kb on - strand at 3758.963 kb on + strand at 3758.963 kb on + strand at 3758.964 kb on - strand at 3758.964 kb on - strand at 3758.964 kb on - strand at 3758.967 kb on - strand at 3758.974 kb on + strand at 3758.974 kb on + strand at 3758.975 kb on - strand at 3758.975 kb on - strand at 3758.975 kb on - strand at 3758.975 kb on - strand at 3759.013 kb on + strand at 3759.014 kb on - strand at 3759.014 kb on - strand at 3759.068 kb on + strand at 3759.111 kb on + strand at 3759.111 kb on + strand at 3759.111 kb on + strand at 3759.111 kb on + strand at 3759.112 kb on - strand at 3759.112 kb on - strand at 3759.112 kb on - strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.119 kb on - strand at 3759.133 kb on - strand at 3759.328 kb on + strand at 3759.561 kb on + strand, within Pf1N1B4_3372 at 3759.561 kb on + strand, within Pf1N1B4_3372 at 3759.562 kb on - strand, within Pf1N1B4_3372 at 3759.589 kb on + strand, within Pf1N1B4_3372 at 3759.606 kb on - strand, within Pf1N1B4_3372 at 3759.683 kb on + strand at 3759.920 kb on - strand at 3760.108 kb on - strand, within Pf1N1B4_3373 at 3760.377 kb on + strand, within Pf1N1B4_3373 at 3760.377 kb on + strand, within Pf1N1B4_3373 at 3760.378 kb on - strand, within Pf1N1B4_3373 at 3760.378 kb on - strand, within Pf1N1B4_3373 at 3760.617 kb on + strand, within Pf1N1B4_3373 at 3760.748 kb on + strand at 3760.748 kb on + strand, within Pf1N1B4_3373 at 3760.748 kb on + strand, within Pf1N1B4_3373 at 3760.748 kb on + strand, within Pf1N1B4_3373 at 3760.818 kb on + strand at 3760.819 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 3,758,377 + Pf1N1B4_3370 0.38 -0.0 3,758,377 + Pf1N1B4_3370 0.38 -0.0 3,758,377 + -0.8 3,758,377 + Pf1N1B4_3370 0.38 -0.0 3,758,377 + Pf1N1B4_3370 0.38 -0.0 3,758,378 - Pf1N1B4_3370 0.38 -2.5 3,758,378 - Pf1N1B4_3370 0.38 -0.0 3,758,435 + Pf1N1B4_3370 0.45 -0.0 3,758,435 + Pf1N1B4_3370 0.45 -1.0 3,758,436 - Pf1N1B4_3370 0.45 -0.0 3,758,436 - Pf1N1B4_3370 0.45 -0.0 3,758,491 + Pf1N1B4_3370 0.51 -0.0 3,758,491 + Pf1N1B4_3370 0.51 -0.0 3,758,491 + Pf1N1B4_3370 0.51 -0.0 3,758,492 - Pf1N1B4_3370 0.51 -0.0 3,758,492 - Pf1N1B4_3370 0.51 -2.1 3,758,492 - Pf1N1B4_3370 0.51 -1.8 3,758,623 + Pf1N1B4_3370 0.67 -0.0 3,758,738 + Pf1N1B4_3370 0.80 -0.0 3,758,878 + -0.9 3,758,879 - -0.0 3,758,879 - -1.3 3,758,957 + -0.0 3,758,957 + -0.8 3,758,958 - -0.0 3,758,958 - -0.0 3,758,958 - -0.0 3,758,963 + -0.0 3,758,963 + -0.0 3,758,964 - -0.0 3,758,964 - -1.1 3,758,964 - -2.2 3,758,967 - -1.7 3,758,974 + -0.0 3,758,974 + -1.9 3,758,975 - -0.0 3,758,975 - -2.7 3,758,975 - -1.7 3,758,975 - -2.2 3,759,013 + -4.3 3,759,014 - -1.7 3,759,014 - -0.0 3,759,068 + -0.0 3,759,111 + -0.9 3,759,111 + -1.6 3,759,111 + -2.4 3,759,111 + -0.0 3,759,112 - -0.0 3,759,112 - -0.0 3,759,112 - -2.1 3,759,114 + -1.4 3,759,114 + -0.0 3,759,114 + -0.0 3,759,114 + -2.6 3,759,114 + -2.2 3,759,114 + -0.0 3,759,114 + -0.0 3,759,114 + -1.3 3,759,114 + -0.0 3,759,114 + -0.0 3,759,114 + -0.0 3,759,115 - -0.9 3,759,115 - -0.0 3,759,115 - -1.7 3,759,115 - -0.0 3,759,115 - -0.0 3,759,115 - -0.0 3,759,115 - -2.0 3,759,115 - -0.0 3,759,115 - -3.0 3,759,115 - -3.1 3,759,115 - -1.5 3,759,119 - -3.3 3,759,133 - -2.7 3,759,328 + -1.9 3,759,561 + Pf1N1B4_3372 0.47 -0.0 3,759,561 + Pf1N1B4_3372 0.47 -0.0 3,759,562 - Pf1N1B4_3372 0.47 -2.7 3,759,589 + Pf1N1B4_3372 0.53 -2.6 3,759,606 - Pf1N1B4_3372 0.56 -0.0 3,759,683 + -0.0 3,759,920 - -0.0 3,760,108 - Pf1N1B4_3373 0.26 -0.0 3,760,377 + Pf1N1B4_3373 0.51 -2.3 3,760,377 + Pf1N1B4_3373 0.51 -0.0 3,760,378 - Pf1N1B4_3373 0.51 -0.2 3,760,378 - Pf1N1B4_3373 0.51 -0.0 3,760,617 + Pf1N1B4_3373 0.74 -0.0 3,760,748 + -1.4 3,760,748 + Pf1N1B4_3373 0.86 -3.9 3,760,748 + Pf1N1B4_3373 0.86 -0.0 3,760,748 + Pf1N1B4_3373 0.86 -0.0 3,760,818 + -1.7 3,760,819 - -0.0
Or see this region's nucleotide sequence