Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3186 and Pf1N1B4_3187 are separated by 114 nucleotides Pf1N1B4_3187 and Pf1N1B4_3188 are separated by 4 nucleotides Pf1N1B4_3188 and Pf1N1B4_3189 are separated by 202 nucleotides
Pf1N1B4_3186 - Conserved secreted protein, at 3,569,331 to 3,569,816
_3186
Pf1N1B4_3187 - UPF0225 protein YchJ, at 3,569,931 to 3,570,407
_3187
Pf1N1B4_3188 - FIG00953202: hypothetical protein, at 3,570,412 to 3,570,909
_3188
Pf1N1B4_3189 - Outer membrane protein A precursor, at 3,571,112 to 3,571,807
_3189
Position (kb) 3570
3571 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 3569.513 kb on + strand, within Pf1N1B4_3186 at 3569.513 kb on + strand, within Pf1N1B4_3186 at 3569.514 kb on - strand, within Pf1N1B4_3186 at 3569.519 kb on - strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.676 kb on + strand, within Pf1N1B4_3186 at 3569.676 kb on + strand, within Pf1N1B4_3186 at 3569.793 kb on + strand at 3569.793 kb on + strand at 3569.793 kb on + strand at 3569.793 kb on + strand at 3569.794 kb on - strand at 3569.824 kb on + strand at 3569.825 kb on - strand at 3569.845 kb on + strand at 3569.845 kb on + strand at 3569.845 kb on + strand at 3569.879 kb on + strand at 3569.879 kb on + strand at 3569.880 kb on - strand at 3569.944 kb on + strand at 3570.000 kb on - strand, within Pf1N1B4_3187 at 3570.000 kb on - strand, within Pf1N1B4_3187 at 3570.000 kb on - strand, within Pf1N1B4_3187 at 3570.037 kb on + strand, within Pf1N1B4_3187 at 3570.037 kb on + strand, within Pf1N1B4_3187 at 3570.038 kb on - strand, within Pf1N1B4_3187 at 3570.038 kb on - strand, within Pf1N1B4_3187 at 3570.058 kb on - strand, within Pf1N1B4_3187 at 3570.261 kb on + strand, within Pf1N1B4_3187 at 3570.261 kb on + strand, within Pf1N1B4_3187 at 3570.261 kb on + strand, within Pf1N1B4_3187 at 3570.261 kb on + strand, within Pf1N1B4_3187 at 3570.262 kb on - strand, within Pf1N1B4_3187 at 3570.262 kb on - strand, within Pf1N1B4_3187 at 3570.262 kb on - strand, within Pf1N1B4_3187 at 3570.262 kb on - strand, within Pf1N1B4_3187 at 3570.265 kb on + strand, within Pf1N1B4_3187 at 3570.266 kb on - strand, within Pf1N1B4_3187 at 3570.282 kb on + strand, within Pf1N1B4_3187 at 3570.282 kb on + strand, within Pf1N1B4_3187 at 3570.282 kb on + strand, within Pf1N1B4_3187 at 3570.282 kb on + strand, within Pf1N1B4_3187 at 3570.282 kb on + strand, within Pf1N1B4_3187 at 3570.282 kb on + strand, within Pf1N1B4_3187 at 3570.282 kb on + strand, within Pf1N1B4_3187 at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.283 kb on - strand at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.286 kb on + strand, within Pf1N1B4_3187 at 3570.286 kb on + strand, within Pf1N1B4_3187 at 3570.287 kb on - strand, within Pf1N1B4_3187 at 3570.287 kb on - strand, within Pf1N1B4_3187 at 3570.287 kb on - strand, within Pf1N1B4_3187 at 3570.287 kb on - strand, within Pf1N1B4_3187 at 3570.346 kb on + strand, within Pf1N1B4_3187 at 3570.401 kb on + strand at 3570.401 kb on + strand at 3570.402 kb on - strand at 3570.402 kb on - strand at 3570.402 kb on - strand at 3570.402 kb on - strand at 3570.402 kb on - strand at 3570.402 kb on - strand at 3570.402 kb on - strand at 3570.402 kb on - strand at 3570.402 kb on - strand at 3570.422 kb on + strand at 3570.422 kb on + strand at 3570.423 kb on - strand at 3570.423 kb on - strand at 3570.483 kb on + strand, within Pf1N1B4_3188 at 3570.483 kb on + strand, within Pf1N1B4_3188 at 3570.484 kb on - strand, within Pf1N1B4_3188 at 3570.484 kb on - strand, within Pf1N1B4_3188 at 3570.484 kb on - strand, within Pf1N1B4_3188 at 3570.485 kb on + strand at 3570.485 kb on + strand, within Pf1N1B4_3188 at 3570.485 kb on + strand, within Pf1N1B4_3188 at 3570.485 kb on + strand, within Pf1N1B4_3188 at 3570.486 kb on - strand, within Pf1N1B4_3188 at 3570.486 kb on - strand, within Pf1N1B4_3188 at 3570.500 kb on + strand, within Pf1N1B4_3188 at 3570.500 kb on + strand, within Pf1N1B4_3188 at 3570.500 kb on + strand, within Pf1N1B4_3188 at 3570.500 kb on + strand, within Pf1N1B4_3188 at 3570.500 kb on + strand, within Pf1N1B4_3188 at 3570.501 kb on - strand, within Pf1N1B4_3188 at 3570.504 kb on + strand, within Pf1N1B4_3188 at 3570.504 kb on + strand, within Pf1N1B4_3188 at 3570.505 kb on - strand, within Pf1N1B4_3188 at 3570.517 kb on + strand, within Pf1N1B4_3188 at 3570.517 kb on + strand, within Pf1N1B4_3188 at 3570.517 kb on + strand, within Pf1N1B4_3188 at 3570.518 kb on - strand, within Pf1N1B4_3188 at 3570.518 kb on - strand, within Pf1N1B4_3188 at 3570.518 kb on - strand, within Pf1N1B4_3188 at 3570.518 kb on - strand, within Pf1N1B4_3188 at 3570.898 kb on - strand at 3570.898 kb on - strand at 3570.898 kb on - strand at 3570.898 kb on - strand at 3570.938 kb on + strand at 3570.961 kb on + strand at 3570.961 kb on + strand at 3570.961 kb on + strand at 3570.961 kb on + strand at 3570.961 kb on + strand at 3570.962 kb on - strand at 3571.085 kb on - strand at 3571.109 kb on + strand at 3571.109 kb on + strand at 3571.109 kb on + strand at 3571.109 kb on + strand at 3571.109 kb on + strand at 3571.109 kb on + strand at 3571.110 kb on - strand at 3571.110 kb on - strand at 3571.110 kb on - strand at 3571.110 kb on - strand at 3571.110 kb on - strand at 3571.110 kb on - strand at 3571.110 kb on - strand at 3571.110 kb on - strand at 3571.119 kb on + strand at 3571.119 kb on + strand at 3571.119 kb on + strand at 3571.119 kb on + strand at 3571.119 kb on + strand at 3571.119 kb on + strand at 3571.119 kb on + strand at 3571.119 kb on + strand at 3571.135 kb on - strand at 3571.135 kb on - strand at 3571.381 kb on + strand, within Pf1N1B4_3189 at 3571.381 kb on + strand, within Pf1N1B4_3189 at 3571.381 kb on + strand, within Pf1N1B4_3189 at 3571.382 kb on - strand, within Pf1N1B4_3189 at 3571.382 kb on - strand at 3571.382 kb on - strand, within Pf1N1B4_3189 at 3571.533 kb on + strand, within Pf1N1B4_3189 at 3571.533 kb on + strand, within Pf1N1B4_3189 at 3571.533 kb on + strand, within Pf1N1B4_3189 at 3571.533 kb on + strand, within Pf1N1B4_3189 at 3571.533 kb on + strand, within Pf1N1B4_3189 at 3571.533 kb on + strand at 3571.533 kb on - strand, within Pf1N1B4_3189 at 3571.534 kb on - strand, within Pf1N1B4_3189 at 3571.625 kb on + strand, within Pf1N1B4_3189 at 3571.625 kb on + strand, within Pf1N1B4_3189 at 3571.625 kb on + strand, within Pf1N1B4_3189 at 3571.625 kb on + strand, within Pf1N1B4_3189 at 3571.625 kb on + strand, within Pf1N1B4_3189 at 3571.625 kb on + strand, within Pf1N1B4_3189 at 3571.626 kb on - strand at 3571.626 kb on - strand, within Pf1N1B4_3189 at 3571.670 kb on + strand, within Pf1N1B4_3189 at 3571.670 kb on + strand, within Pf1N1B4_3189 at 3571.670 kb on + strand, within Pf1N1B4_3189 at 3571.671 kb on - strand, within Pf1N1B4_3189 at 3571.671 kb on - strand at 3571.671 kb on - strand, within Pf1N1B4_3189 at 3571.699 kb on + strand, within Pf1N1B4_3189 at 3571.699 kb on + strand, within Pf1N1B4_3189 at 3571.699 kb on + strand, within Pf1N1B4_3189 at 3571.700 kb on - strand, within Pf1N1B4_3189 at 3571.700 kb on - strand, within Pf1N1B4_3189 at 3571.700 kb on - strand, within Pf1N1B4_3189 at 3571.700 kb on - strand, within Pf1N1B4_3189 at 3571.805 kb on + strand at 3571.830 kb on + strand at 3571.830 kb on + strand at 3571.830 kb on + strand at 3571.830 kb on + strand at 3571.830 kb on + strand at 3571.830 kb on + strand at 3571.830 kb on + strand at 3571.831 kb on - strand at 3571.831 kb on - strand at 3571.831 kb on - strand at 3571.831 kb on - strand at 3571.852 kb on + strand at 3571.852 kb on + strand at 3571.852 kb on + strand at 3571.909 kb on + strand at 3571.909 kb on + strand at 3571.909 kb on + strand at 3571.909 kb on + strand at 3571.909 kb on + strand at 3571.909 kb on + strand at 3571.909 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 3,569,513 + Pf1N1B4_3186 0.37 -2.4 3,569,513 + Pf1N1B4_3186 0.37 +0.0 3,569,514 - Pf1N1B4_3186 0.38 -3.0 3,569,519 - Pf1N1B4_3186 0.39 -1.0 3,569,577 + Pf1N1B4_3186 0.51 +0.0 3,569,577 + Pf1N1B4_3186 0.51 +0.0 3,569,577 + Pf1N1B4_3186 0.51 +0.0 3,569,577 + Pf1N1B4_3186 0.51 -1.0 3,569,577 + Pf1N1B4_3186 0.51 -2.0 3,569,577 + Pf1N1B4_3186 0.51 -0.8 3,569,577 + Pf1N1B4_3186 0.51 +0.0 3,569,577 + Pf1N1B4_3186 0.51 +0.0 3,569,577 + Pf1N1B4_3186 0.51 -2.2 3,569,577 + Pf1N1B4_3186 0.51 -2.1 3,569,577 + Pf1N1B4_3186 0.51 +0.0 3,569,577 + Pf1N1B4_3186 0.51 +0.0 3,569,577 + Pf1N1B4_3186 0.51 +0.0 3,569,577 + Pf1N1B4_3186 0.51 -1.5 3,569,577 + Pf1N1B4_3186 0.51 -1.2 3,569,578 - Pf1N1B4_3186 0.51 +0.0 3,569,578 - Pf1N1B4_3186 0.51 -2.6 3,569,578 - Pf1N1B4_3186 0.51 -1.5 3,569,578 - Pf1N1B4_3186 0.51 +0.0 3,569,578 - Pf1N1B4_3186 0.51 +0.0 3,569,578 - Pf1N1B4_3186 0.51 -1.6 3,569,578 - Pf1N1B4_3186 0.51 +0.0 3,569,578 - Pf1N1B4_3186 0.51 -2.4 3,569,676 + Pf1N1B4_3186 0.71 -1.3 3,569,676 + Pf1N1B4_3186 0.71 -1.9 3,569,793 + -1.7 3,569,793 + -0.7 3,569,793 + -2.1 3,569,793 + +0.0 3,569,794 - +0.0 3,569,824 + +0.0 3,569,825 - +0.0 3,569,845 + -2.0 3,569,845 + -0.6 3,569,845 + +0.0 3,569,879 + -2.1 3,569,879 + +0.0 3,569,880 - -1.3 3,569,944 + +0.0 3,570,000 - Pf1N1B4_3187 0.14 -1.2 3,570,000 - Pf1N1B4_3187 0.14 -2.4 3,570,000 - Pf1N1B4_3187 0.14 +0.0 3,570,037 + Pf1N1B4_3187 0.22 +0.0 3,570,037 + Pf1N1B4_3187 0.22 +0.0 3,570,038 - Pf1N1B4_3187 0.22 -1.5 3,570,038 - Pf1N1B4_3187 0.22 +0.0 3,570,058 - Pf1N1B4_3187 0.27 +0.0 3,570,261 + Pf1N1B4_3187 0.69 -1.7 3,570,261 + Pf1N1B4_3187 0.69 -0.8 3,570,261 + Pf1N1B4_3187 0.69 -0.3 3,570,261 + Pf1N1B4_3187 0.69 -1.7 3,570,262 - Pf1N1B4_3187 0.69 +0.0 3,570,262 - Pf1N1B4_3187 0.69 -1.2 3,570,262 - Pf1N1B4_3187 0.69 +0.0 3,570,262 - Pf1N1B4_3187 0.69 +0.0 3,570,265 + Pf1N1B4_3187 0.70 +0.0 3,570,266 - Pf1N1B4_3187 0.70 -1.6 3,570,282 + Pf1N1B4_3187 0.74 +0.0 3,570,282 + Pf1N1B4_3187 0.74 -2.6 3,570,282 + Pf1N1B4_3187 0.74 +0.0 3,570,282 + Pf1N1B4_3187 0.74 +0.0 3,570,282 + Pf1N1B4_3187 0.74 -2.1 3,570,282 + Pf1N1B4_3187 0.74 +0.0 3,570,282 + Pf1N1B4_3187 0.74 +0.0 3,570,283 - Pf1N1B4_3187 0.74 +0.0 3,570,283 - +0.0 3,570,283 - Pf1N1B4_3187 0.74 +0.0 3,570,283 - Pf1N1B4_3187 0.74 +0.0 3,570,283 - Pf1N1B4_3187 0.74 +0.0 3,570,283 - Pf1N1B4_3187 0.74 -0.9 3,570,283 - Pf1N1B4_3187 0.74 +0.0 3,570,283 - Pf1N1B4_3187 0.74 +0.0 3,570,283 - Pf1N1B4_3187 0.74 -2.4 3,570,286 + Pf1N1B4_3187 0.74 -0.6 3,570,286 + Pf1N1B4_3187 0.74 +0.0 3,570,287 - Pf1N1B4_3187 0.75 +0.0 3,570,287 - Pf1N1B4_3187 0.75 +0.0 3,570,287 - Pf1N1B4_3187 0.75 -2.2 3,570,287 - Pf1N1B4_3187 0.75 +0.0 3,570,346 + Pf1N1B4_3187 0.87 +0.0 3,570,401 + +0.0 3,570,401 + -2.3 3,570,402 - +0.0 3,570,402 - +0.0 3,570,402 - +0.0 3,570,402 - +0.0 3,570,402 - -0.9 3,570,402 - -2.0 3,570,402 - +0.0 3,570,402 - +0.0 3,570,402 - +0.0 3,570,422 + +0.0 3,570,422 + -2.9 3,570,423 - -0.6 3,570,423 - +0.0 3,570,483 + Pf1N1B4_3188 0.14 -4.2 3,570,483 + Pf1N1B4_3188 0.14 -1.4 3,570,484 - Pf1N1B4_3188 0.14 +0.0 3,570,484 - Pf1N1B4_3188 0.14 -1.6 3,570,484 - Pf1N1B4_3188 0.14 -2.4 3,570,485 + +0.0 3,570,485 + Pf1N1B4_3188 0.15 +0.0 3,570,485 + Pf1N1B4_3188 0.15 -1.2 3,570,485 + Pf1N1B4_3188 0.15 +0.0 3,570,486 - Pf1N1B4_3188 0.15 +0.0 3,570,486 - Pf1N1B4_3188 0.15 -2.3 3,570,500 + Pf1N1B4_3188 0.18 +0.0 3,570,500 + Pf1N1B4_3188 0.18 +0.0 3,570,500 + Pf1N1B4_3188 0.18 +0.0 3,570,500 + Pf1N1B4_3188 0.18 +0.0 3,570,500 + Pf1N1B4_3188 0.18 -1.5 3,570,501 - Pf1N1B4_3188 0.18 +0.0 3,570,504 + Pf1N1B4_3188 0.18 -1.7 3,570,504 + Pf1N1B4_3188 0.18 -0.2 3,570,505 - Pf1N1B4_3188 0.19 +0.0 3,570,517 + Pf1N1B4_3188 0.21 +0.0 3,570,517 + Pf1N1B4_3188 0.21 +0.0 3,570,517 + Pf1N1B4_3188 0.21 -1.6 3,570,518 - Pf1N1B4_3188 0.21 +0.0 3,570,518 - Pf1N1B4_3188 0.21 -1.0 3,570,518 - Pf1N1B4_3188 0.21 +0.0 3,570,518 - Pf1N1B4_3188 0.21 +0.0 3,570,898 - +0.0 3,570,898 - +0.0 3,570,898 - +0.0 3,570,898 - -1.6 3,570,938 + +0.0 3,570,961 + -1.4 3,570,961 + +0.0 3,570,961 + -1.8 3,570,961 + -1.9 3,570,961 + +0.0 3,570,962 - -0.7 3,571,085 - -1.3 3,571,109 + +0.0 3,571,109 + +0.0 3,571,109 + -1.3 3,571,109 + -1.7 3,571,109 + -1.4 3,571,109 + +0.0 3,571,110 - -2.2 3,571,110 - -2.4 3,571,110 - +0.0 3,571,110 - -1.0 3,571,110 - -2.7 3,571,110 - +0.0 3,571,110 - +0.0 3,571,110 - +0.0 3,571,119 + -1.7 3,571,119 + +0.0 3,571,119 + -1.9 3,571,119 + +0.0 3,571,119 + +0.0 3,571,119 + -1.6 3,571,119 + +0.0 3,571,119 + +0.0 3,571,135 - -2.5 3,571,135 - +0.0 3,571,381 + Pf1N1B4_3189 0.39 +0.0 3,571,381 + Pf1N1B4_3189 0.39 +0.0 3,571,381 + Pf1N1B4_3189 0.39 +0.0 3,571,382 - Pf1N1B4_3189 0.39 +0.0 3,571,382 - +0.0 3,571,382 - Pf1N1B4_3189 0.39 -0.9 3,571,533 + Pf1N1B4_3189 0.60 +0.0 3,571,533 + Pf1N1B4_3189 0.60 +0.0 3,571,533 + Pf1N1B4_3189 0.60 +0.0 3,571,533 + Pf1N1B4_3189 0.60 +0.0 3,571,533 + Pf1N1B4_3189 0.60 +0.0 3,571,533 + +0.0 3,571,533 - Pf1N1B4_3189 0.60 -1.3 3,571,534 - Pf1N1B4_3189 0.61 +0.0 3,571,625 + Pf1N1B4_3189 0.74 +0.0 3,571,625 + Pf1N1B4_3189 0.74 +0.0 3,571,625 + Pf1N1B4_3189 0.74 +0.0 3,571,625 + Pf1N1B4_3189 0.74 +0.0 3,571,625 + Pf1N1B4_3189 0.74 -1.8 3,571,625 + Pf1N1B4_3189 0.74 +0.0 3,571,626 - +0.0 3,571,626 - Pf1N1B4_3189 0.74 -2.3 3,571,670 + Pf1N1B4_3189 0.80 +0.0 3,571,670 + Pf1N1B4_3189 0.80 +0.0 3,571,670 + Pf1N1B4_3189 0.80 +0.0 3,571,671 - Pf1N1B4_3189 0.80 -2.1 3,571,671 - +0.0 3,571,671 - Pf1N1B4_3189 0.80 +0.0 3,571,699 + Pf1N1B4_3189 0.84 +0.0 3,571,699 + Pf1N1B4_3189 0.84 -1.6 3,571,699 + Pf1N1B4_3189 0.84 -2.1 3,571,700 - Pf1N1B4_3189 0.84 +0.0 3,571,700 - Pf1N1B4_3189 0.84 +0.0 3,571,700 - Pf1N1B4_3189 0.84 +0.0 3,571,700 - Pf1N1B4_3189 0.84 +0.0 3,571,805 + +0.0 3,571,830 + +0.0 3,571,830 + +0.0 3,571,830 + +0.0 3,571,830 + -3.1 3,571,830 + +0.0 3,571,830 + +0.0 3,571,830 + +0.0 3,571,831 - -1.1 3,571,831 - +0.0 3,571,831 - -2.9 3,571,831 - -1.6 3,571,852 + +0.0 3,571,852 + -1.4 3,571,852 + -1.6 3,571,909 + -1.8 3,571,909 + +0.0 3,571,909 + +0.0 3,571,909 + -1.5 3,571,909 + +0.0 3,571,909 + -2.1 3,571,909 + +0.0
Or see this region's nucleotide sequence