Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3133 and Pf1N1B4_3134 overlap by 4 nucleotides Pf1N1B4_3134 and Pf1N1B4_3135 are separated by 137 nucleotides
Pf1N1B4_3133 - Prophage tail fiber protein, at 3,517,639 to 3,518,787
_3133
Pf1N1B4_3134 - probable tail fiber assembly-like protein, at 3,518,784 to 3,519,299
_3134
Pf1N1B4_3135 - Prophage tail fiber protein, at 3,519,437 to 3,520,477
_3135
Position (kb)
3518
3519
3520 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3517.813 kb on + strand at 3517.813 kb on + strand, within Pf1N1B4_3133 at 3517.813 kb on + strand, within Pf1N1B4_3133 at 3517.813 kb on + strand, within Pf1N1B4_3133 at 3517.814 kb on - strand, within Pf1N1B4_3133 at 3518.090 kb on - strand at 3518.131 kb on + strand, within Pf1N1B4_3133 at 3518.131 kb on + strand, within Pf1N1B4_3133 at 3518.131 kb on + strand, within Pf1N1B4_3133 at 3518.131 kb on + strand, within Pf1N1B4_3133 at 3518.131 kb on + strand, within Pf1N1B4_3133 at 3518.131 kb on + strand, within Pf1N1B4_3133 at 3518.131 kb on + strand, within Pf1N1B4_3133 at 3518.132 kb on - strand, within Pf1N1B4_3133 at 3518.152 kb on + strand, within Pf1N1B4_3133 at 3518.152 kb on + strand, within Pf1N1B4_3133 at 3518.152 kb on + strand, within Pf1N1B4_3133 at 3518.152 kb on + strand, within Pf1N1B4_3133 at 3518.152 kb on + strand, within Pf1N1B4_3133 at 3518.152 kb on + strand, within Pf1N1B4_3133 at 3518.152 kb on + strand, within Pf1N1B4_3133 at 3518.153 kb on - strand, within Pf1N1B4_3133 at 3518.290 kb on + strand at 3518.291 kb on - strand, within Pf1N1B4_3133 at 3518.291 kb on - strand, within Pf1N1B4_3133 at 3518.344 kb on + strand, within Pf1N1B4_3133 at 3518.344 kb on + strand, within Pf1N1B4_3133 at 3518.345 kb on - strand, within Pf1N1B4_3133 at 3518.345 kb on - strand, within Pf1N1B4_3133 at 3518.461 kb on + strand, within Pf1N1B4_3133 at 3518.461 kb on + strand, within Pf1N1B4_3133 at 3518.541 kb on + strand, within Pf1N1B4_3133 at 3518.548 kb on - strand, within Pf1N1B4_3133 at 3518.627 kb on - strand, within Pf1N1B4_3133 at 3518.685 kb on + strand at 3518.716 kb on + strand at 3518.717 kb on - strand at 3518.717 kb on - strand at 3518.792 kb on + strand at 3518.824 kb on + strand at 3518.824 kb on + strand at 3518.824 kb on + strand at 3518.824 kb on + strand at 3518.824 kb on + strand at 3518.825 kb on - strand at 3518.825 kb on - strand at 3519.000 kb on + strand, within Pf1N1B4_3134 at 3519.000 kb on + strand, within Pf1N1B4_3134 at 3519.000 kb on + strand, within Pf1N1B4_3134 at 3519.326 kb on + strand at 3519.326 kb on + strand at 3519.326 kb on + strand at 3519.326 kb on + strand at 3519.327 kb on - strand at 3519.414 kb on + strand at 3519.415 kb on - strand at 3519.436 kb on + strand at 3519.436 kb on + strand at 3519.436 kb on + strand at 3519.437 kb on - strand at 3519.577 kb on + strand, within Pf1N1B4_3135 at 3519.578 kb on - strand at 3519.684 kb on + strand, within Pf1N1B4_3135 at 3519.685 kb on - strand, within Pf1N1B4_3135 at 3519.685 kb on - strand, within Pf1N1B4_3135 at 3519.733 kb on + strand, within Pf1N1B4_3135 at 3519.840 kb on + strand, within Pf1N1B4_3135 at 3519.840 kb on + strand, within Pf1N1B4_3135 at 3519.843 kb on + strand, within Pf1N1B4_3135 at 3519.843 kb on + strand, within Pf1N1B4_3135 at 3519.844 kb on - strand, within Pf1N1B4_3135 at 3519.942 kb on + strand, within Pf1N1B4_3135 at 3519.942 kb on + strand, within Pf1N1B4_3135 at 3519.942 kb on + strand, within Pf1N1B4_3135 at 3519.943 kb on - strand, within Pf1N1B4_3135
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 3,517,813 + -2.7 3,517,813 + Pf1N1B4_3133 0.15 -2.4 3,517,813 + Pf1N1B4_3133 0.15 +0.0 3,517,813 + Pf1N1B4_3133 0.15 +0.0 3,517,814 - Pf1N1B4_3133 0.15 +0.0 3,518,090 - -1.5 3,518,131 + Pf1N1B4_3133 0.43 +0.0 3,518,131 + Pf1N1B4_3133 0.43 -2.2 3,518,131 + Pf1N1B4_3133 0.43 +0.0 3,518,131 + Pf1N1B4_3133 0.43 +0.0 3,518,131 + Pf1N1B4_3133 0.43 -1.2 3,518,131 + Pf1N1B4_3133 0.43 +0.0 3,518,131 + Pf1N1B4_3133 0.43 -1.1 3,518,132 - Pf1N1B4_3133 0.43 +0.0 3,518,152 + Pf1N1B4_3133 0.45 -1.6 3,518,152 + Pf1N1B4_3133 0.45 -1.6 3,518,152 + Pf1N1B4_3133 0.45 +0.0 3,518,152 + Pf1N1B4_3133 0.45 -1.8 3,518,152 + Pf1N1B4_3133 0.45 +0.0 3,518,152 + Pf1N1B4_3133 0.45 +0.0 3,518,152 + Pf1N1B4_3133 0.45 +0.0 3,518,153 - Pf1N1B4_3133 0.45 +0.0 3,518,290 + +0.0 3,518,291 - Pf1N1B4_3133 0.57 +0.0 3,518,291 - Pf1N1B4_3133 0.57 +0.0 3,518,344 + Pf1N1B4_3133 0.61 +0.0 3,518,344 + Pf1N1B4_3133 0.61 -2.5 3,518,345 - Pf1N1B4_3133 0.61 +0.0 3,518,345 - Pf1N1B4_3133 0.61 +0.0 3,518,461 + Pf1N1B4_3133 0.72 +0.0 3,518,461 + Pf1N1B4_3133 0.72 -2.3 3,518,541 + Pf1N1B4_3133 0.79 +0.0 3,518,548 - Pf1N1B4_3133 0.79 +0.0 3,518,627 - Pf1N1B4_3133 0.86 -2.1 3,518,685 + +0.0 3,518,716 + -1.3 3,518,717 - +0.0 3,518,717 - -2.7 3,518,792 + -1.0 3,518,824 + +0.0 3,518,824 + -2.0 3,518,824 + +0.1 3,518,824 + -2.4 3,518,824 + +0.0 3,518,825 - -0.9 3,518,825 - -1.2 3,519,000 + Pf1N1B4_3134 0.42 -2.6 3,519,000 + Pf1N1B4_3134 0.42 +0.0 3,519,000 + Pf1N1B4_3134 0.42 +0.0 3,519,326 + +0.0 3,519,326 + -1.6 3,519,326 + -1.0 3,519,326 + +0.0 3,519,327 - +0.0 3,519,414 + +0.0 3,519,415 - -2.4 3,519,436 + +0.0 3,519,436 + +0.0 3,519,436 + +0.0 3,519,437 - -1.5 3,519,577 + Pf1N1B4_3135 0.13 +0.0 3,519,578 - +0.0 3,519,684 + Pf1N1B4_3135 0.24 +0.0 3,519,685 - Pf1N1B4_3135 0.24 +0.0 3,519,685 - Pf1N1B4_3135 0.24 +0.0 3,519,733 + Pf1N1B4_3135 0.28 +0.0 3,519,840 + Pf1N1B4_3135 0.39 -3.5 3,519,840 + Pf1N1B4_3135 0.39 +0.0 3,519,843 + Pf1N1B4_3135 0.39 -2.4 3,519,843 + Pf1N1B4_3135 0.39 -1.0 3,519,844 - Pf1N1B4_3135 0.39 +0.0 3,519,942 + Pf1N1B4_3135 0.49 -1.9 3,519,942 + Pf1N1B4_3135 0.49 -1.6 3,519,942 + Pf1N1B4_3135 0.49 -1.4 3,519,943 - Pf1N1B4_3135 0.49 +0.0
Or see this region's nucleotide sequence