Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3090 and Pf1N1B4_3091 are separated by 67 nucleotides Pf1N1B4_3091 and Pf1N1B4_3092 are separated by 72 nucleotides
Pf1N1B4_3090 - 1-deoxy-D-xylulose 5-phosphate reductoisomerase (EC 1.1.1.267), at 3,472,618 to 3,473,808
_3090
Pf1N1B4_3091 - Membrane-associated zinc metalloprotease, at 3,473,876 to 3,475,213
_3091
Pf1N1B4_3092 - Outer membrane protein assembly factor YaeT precursor, at 3,475,286 to 3,477,676
_3092
Position (kb)
3473
3474
3475
3476 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3473.806 kb on + strand at 3473.818 kb on + strand at 3473.818 kb on + strand at 3473.818 kb on + strand at 3473.818 kb on + strand at 3473.818 kb on + strand at 3473.818 kb on + strand at 3473.818 kb on + strand at 3473.818 kb on + strand at 3473.818 kb on + strand at 3473.818 kb on + strand at 3473.841 kb on + strand at 3473.842 kb on - strand at 3473.858 kb on + strand at 3473.858 kb on + strand at 3473.858 kb on + strand at 3473.859 kb on - strand at 3473.859 kb on - strand at 3473.859 kb on - strand at 3473.873 kb on + strand at 3473.873 kb on + strand at 3473.873 kb on + strand at 3473.873 kb on + strand at 3473.873 kb on + strand at 3473.873 kb on + strand at 3473.873 kb on + strand at 3473.873 kb on + strand at 3473.874 kb on - strand at 3473.874 kb on - strand at 3473.874 kb on - strand at 3473.874 kb on - strand at 3473.874 kb on - strand at 3473.874 kb on - strand at 3473.875 kb on + strand at 3473.875 kb on + strand at 3473.875 kb on + strand at 3473.875 kb on + strand at 3473.875 kb on + strand at 3473.875 kb on + strand at 3473.875 kb on + strand at 3473.875 kb on + strand at 3473.876 kb on - strand at 3473.876 kb on - strand at 3473.876 kb on - strand at 3473.876 kb on - strand at 3473.876 kb on - strand at 3474.125 kb on - strand, within Pf1N1B4_3091 at 3474.186 kb on + strand, within Pf1N1B4_3091 at 3474.640 kb on - strand, within Pf1N1B4_3091 at 3474.656 kb on - strand, within Pf1N1B4_3091 at 3474.876 kb on + strand, within Pf1N1B4_3091 at 3474.876 kb on + strand, within Pf1N1B4_3091 at 3475.013 kb on + strand, within Pf1N1B4_3091 at 3475.013 kb on + strand, within Pf1N1B4_3091 at 3475.013 kb on + strand, within Pf1N1B4_3091 at 3475.021 kb on + strand at 3475.021 kb on + strand, within Pf1N1B4_3091 at 3475.085 kb on + strand at 3475.085 kb on + strand at 3475.179 kb on + strand at 3475.179 kb on + strand at 3475.179 kb on + strand at 3475.179 kb on + strand at 3475.179 kb on + strand at 3475.179 kb on + strand at 3475.179 kb on + strand at 3475.179 kb on + strand at 3475.179 kb on + strand at 3475.179 kb on + strand at 3475.179 kb on + strand at 3475.179 kb on + strand at 3475.211 kb on + strand at 3475.211 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 3,473,806 + +0.0 3,473,818 + +0.0 3,473,818 + +0.0 3,473,818 + +0.0 3,473,818 + -2.9 3,473,818 + -1.4 3,473,818 + +0.0 3,473,818 + +0.0 3,473,818 + +0.0 3,473,818 + -0.9 3,473,818 + +0.0 3,473,841 + +0.0 3,473,842 - -2.3 3,473,858 + +0.0 3,473,858 + -1.2 3,473,858 + +0.0 3,473,859 - -2.5 3,473,859 - -1.9 3,473,859 - +0.0 3,473,873 + -1.2 3,473,873 + +0.0 3,473,873 + +0.0 3,473,873 + -1.7 3,473,873 + -1.2 3,473,873 + -2.5 3,473,873 + +0.0 3,473,873 + +0.0 3,473,874 - +0.0 3,473,874 - -1.7 3,473,874 - +0.0 3,473,874 - +1.2 3,473,874 - -3.6 3,473,874 - +0.0 3,473,875 + +0.0 3,473,875 + +0.0 3,473,875 + -2.6 3,473,875 + +0.0 3,473,875 + +0.0 3,473,875 + +0.0 3,473,875 + +0.0 3,473,875 + +0.0 3,473,876 - -1.8 3,473,876 - -3.3 3,473,876 - +0.0 3,473,876 - -3.2 3,473,876 - -2.7 3,474,125 - Pf1N1B4_3091 0.19 +0.0 3,474,186 + Pf1N1B4_3091 0.23 +0.0 3,474,640 - Pf1N1B4_3091 0.57 -4.2 3,474,656 - Pf1N1B4_3091 0.58 -2.6 3,474,876 + Pf1N1B4_3091 0.75 -2.2 3,474,876 + Pf1N1B4_3091 0.75 +0.0 3,475,013 + Pf1N1B4_3091 0.85 -2.6 3,475,013 + Pf1N1B4_3091 0.85 +0.0 3,475,013 + Pf1N1B4_3091 0.85 +0.0 3,475,021 + +0.8 3,475,021 + Pf1N1B4_3091 0.86 -2.1 3,475,085 + +0.0 3,475,085 + +0.0 3,475,179 + -2.2 3,475,179 + +0.0 3,475,179 + +0.0 3,475,179 + -2.9 3,475,179 + +0.0 3,475,179 + +0.0 3,475,179 + +0.0 3,475,179 + +0.0 3,475,179 + -2.9 3,475,179 + -4.6 3,475,179 + +0.0 3,475,179 + +0.0 3,475,211 + +0.0 3,475,211 + -1.0
Or see this region's nucleotide sequence