Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3067 and Pf1N1B4_3068 are separated by 165 nucleotides Pf1N1B4_3068 and Pf1N1B4_3069 are separated by 116 nucleotides Pf1N1B4_3069 and Pf1N1B4_3070 are separated by 84 nucleotides Pf1N1B4_3070 and Pf1N1B4_3071 overlap by 1 nucleotides
Pf1N1B4_3067 - Glycerol-3-phosphate acyltransferase (EC 2.3.1.15), at 3,448,727 to 3,451,210
_3067
Pf1N1B4_3068 - Cold shock protein CspA, at 3,451,376 to 3,451,585
_3068
Pf1N1B4_3069 - FIG00954614: hypothetical protein, at 3,451,702 to 3,452,079
_3069
Pf1N1B4_3070 - Ribosomal RNA large subunit methyltransferase A (EC 2.1.1.51), at 3,452,164 to 3,452,973
_3070
Pf1N1B4_3071 - N-succinyl-L,L-diaminopimelate desuccinylase (EC 3.5.1.18), at 3,452,973 to 3,454,124
_3071
Position (kb)
3452
3453 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3451.260 kb on + strand at 3451.261 kb on - strand at 3451.299 kb on + strand at 3451.303 kb on + strand at 3451.303 kb on + strand at 3451.303 kb on + strand at 3451.304 kb on - strand at 3451.304 kb on - strand at 3451.335 kb on + strand at 3451.336 kb on - strand at 3451.337 kb on + strand at 3451.337 kb on + strand at 3451.337 kb on + strand at 3451.337 kb on + strand at 3451.338 kb on - strand at 3451.354 kb on + strand at 3451.367 kb on + strand at 3451.368 kb on - strand at 3451.371 kb on + strand at 3451.371 kb on + strand at 3451.371 kb on + strand at 3451.371 kb on + strand at 3451.371 kb on + strand at 3451.371 kb on + strand at 3451.371 kb on + strand at 3451.372 kb on - strand at 3451.372 kb on - strand at 3451.372 kb on - strand at 3451.372 kb on - strand at 3451.372 kb on - strand at 3451.372 kb on - strand at 3451.372 kb on - strand at 3451.372 kb on - strand at 3451.372 kb on - strand at 3451.376 kb on - strand at 3451.427 kb on + strand, within Pf1N1B4_3068 at 3451.472 kb on + strand, within Pf1N1B4_3068 at 3451.529 kb on + strand, within Pf1N1B4_3068 at 3451.529 kb on + strand, within Pf1N1B4_3068 at 3451.575 kb on + strand at 3451.575 kb on + strand at 3451.575 kb on + strand at 3451.575 kb on + strand at 3451.576 kb on - strand at 3451.576 kb on - strand at 3451.576 kb on - strand at 3451.576 kb on - strand at 3451.576 kb on - strand at 3451.576 kb on - strand at 3451.958 kb on + strand, within Pf1N1B4_3069 at 3451.958 kb on + strand, within Pf1N1B4_3069 at 3451.959 kb on - strand, within Pf1N1B4_3069 at 3451.959 kb on - strand, within Pf1N1B4_3069 at 3452.030 kb on + strand, within Pf1N1B4_3069 at 3452.030 kb on + strand, within Pf1N1B4_3069 at 3452.030 kb on + strand, within Pf1N1B4_3069 at 3452.031 kb on - strand, within Pf1N1B4_3069 at 3452.054 kb on + strand at 3452.181 kb on - strand at 3452.411 kb on + strand, within Pf1N1B4_3070 at 3452.412 kb on - strand, within Pf1N1B4_3070 at 3452.412 kb on - strand, within Pf1N1B4_3070 at 3452.481 kb on + strand, within Pf1N1B4_3070 at 3452.482 kb on - strand, within Pf1N1B4_3070 at 3452.690 kb on + strand, within Pf1N1B4_3070 at 3452.690 kb on + strand, within Pf1N1B4_3070 at 3452.690 kb on + strand, within Pf1N1B4_3070 at 3452.690 kb on + strand, within Pf1N1B4_3070 at 3452.690 kb on + strand, within Pf1N1B4_3070 at 3452.691 kb on - strand, within Pf1N1B4_3070 at 3452.691 kb on - strand, within Pf1N1B4_3070 at 3452.693 kb on + strand, within Pf1N1B4_3070 at 3452.693 kb on + strand, within Pf1N1B4_3070 at 3452.694 kb on - strand, within Pf1N1B4_3070 at 3452.694 kb on - strand, within Pf1N1B4_3070 at 3452.736 kb on - strand, within Pf1N1B4_3070 at 3452.778 kb on - strand, within Pf1N1B4_3070 at 3452.850 kb on + strand, within Pf1N1B4_3070 at 3452.850 kb on + strand, within Pf1N1B4_3070 at 3452.850 kb on + strand at 3452.851 kb on - strand, within Pf1N1B4_3070 at 3452.851 kb on - strand, within Pf1N1B4_3070 at 3452.851 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 3,451,260 + -1.8 3,451,261 - +0.0 3,451,299 + +0.0 3,451,303 + -1.8 3,451,303 + +0.0 3,451,303 + -0.4 3,451,304 - +0.0 3,451,304 - -2.6 3,451,335 + -1.1 3,451,336 - +0.0 3,451,337 + +0.0 3,451,337 + +0.0 3,451,337 + -1.1 3,451,337 + -2.0 3,451,338 - +0.0 3,451,354 + +0.0 3,451,367 + +0.0 3,451,368 - +0.0 3,451,371 + +0.0 3,451,371 + -1.8 3,451,371 + +0.0 3,451,371 + -2.0 3,451,371 + -0.5 3,451,371 + +0.0 3,451,371 + +0.0 3,451,372 - +0.0 3,451,372 - +0.0 3,451,372 - +0.0 3,451,372 - +0.0 3,451,372 - -0.4 3,451,372 - -0.7 3,451,372 - +0.0 3,451,372 - -1.2 3,451,372 - -0.5 3,451,376 - +0.0 3,451,427 + Pf1N1B4_3068 0.24 -1.5 3,451,472 + Pf1N1B4_3068 0.46 +0.0 3,451,529 + Pf1N1B4_3068 0.73 +0.0 3,451,529 + Pf1N1B4_3068 0.73 -0.4 3,451,575 + -1.9 3,451,575 + +0.0 3,451,575 + -1.4 3,451,575 + -1.3 3,451,576 - +0.0 3,451,576 - +0.0 3,451,576 - -2.9 3,451,576 - +0.0 3,451,576 - +0.0 3,451,576 - +0.0 3,451,958 + Pf1N1B4_3069 0.68 +1.0 3,451,958 + Pf1N1B4_3069 0.68 +0.0 3,451,959 - Pf1N1B4_3069 0.68 -2.7 3,451,959 - Pf1N1B4_3069 0.68 +0.0 3,452,030 + Pf1N1B4_3069 0.87 +0.0 3,452,030 + Pf1N1B4_3069 0.87 +0.0 3,452,030 + Pf1N1B4_3069 0.87 -1.3 3,452,031 - Pf1N1B4_3069 0.87 +0.0 3,452,054 + -0.7 3,452,181 - +0.0 3,452,411 + Pf1N1B4_3070 0.30 +0.0 3,452,412 - Pf1N1B4_3070 0.31 -2.2 3,452,412 - Pf1N1B4_3070 0.31 +0.0 3,452,481 + Pf1N1B4_3070 0.39 +0.0 3,452,482 - Pf1N1B4_3070 0.39 +0.0 3,452,690 + Pf1N1B4_3070 0.65 +0.0 3,452,690 + Pf1N1B4_3070 0.65 +0.0 3,452,690 + Pf1N1B4_3070 0.65 -2.7 3,452,690 + Pf1N1B4_3070 0.65 -3.2 3,452,690 + Pf1N1B4_3070 0.65 +0.0 3,452,691 - Pf1N1B4_3070 0.65 +0.0 3,452,691 - Pf1N1B4_3070 0.65 +0.0 3,452,693 + Pf1N1B4_3070 0.65 +0.0 3,452,693 + Pf1N1B4_3070 0.65 +0.0 3,452,694 - Pf1N1B4_3070 0.65 -1.0 3,452,694 - Pf1N1B4_3070 0.65 +0.0 3,452,736 - Pf1N1B4_3070 0.71 +0.0 3,452,778 - Pf1N1B4_3070 0.76 +0.0 3,452,850 + Pf1N1B4_3070 0.85 +0.0 3,452,850 + Pf1N1B4_3070 0.85 -2.0 3,452,850 + +0.0 3,452,851 - Pf1N1B4_3070 0.85 +0.0 3,452,851 - Pf1N1B4_3070 0.85 -2.0 3,452,851 - +0.0
Or see this region's nucleotide sequence