Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3022 and Pf1N1B4_3023 are separated by 411 nucleotides Pf1N1B4_3023 and Pf1N1B4_3024 are separated by 133 nucleotides
Pf1N1B4_3022 - Glucans biosynthesis protein D precursor, at 3,398,598 to 3,400,223
_3022
Pf1N1B4_3023 - hypothetical protein, at 3,400,635 to 3,400,820
_3023
Pf1N1B4_3024 - 4-aminobutyraldehyde dehydrogenase (EC 1.2.1.19), at 3,400,954 to 3,402,378
_3024
Position (kb)
3400
3401 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3399.694 kb on + strand, within Pf1N1B4_3022 at 3399.694 kb on + strand, within Pf1N1B4_3022 at 3399.695 kb on - strand, within Pf1N1B4_3022 at 3399.695 kb on - strand, within Pf1N1B4_3022 at 3399.695 kb on - strand, within Pf1N1B4_3022 at 3399.791 kb on + strand, within Pf1N1B4_3022 at 3399.792 kb on - strand at 3399.814 kb on + strand, within Pf1N1B4_3022 at 3399.877 kb on + strand, within Pf1N1B4_3022 at 3399.877 kb on + strand, within Pf1N1B4_3022 at 3399.877 kb on + strand, within Pf1N1B4_3022 at 3399.877 kb on + strand, within Pf1N1B4_3022 at 3399.877 kb on + strand, within Pf1N1B4_3022 at 3399.878 kb on - strand, within Pf1N1B4_3022 at 3399.878 kb on - strand, within Pf1N1B4_3022 at 3399.878 kb on - strand, within Pf1N1B4_3022 at 3399.878 kb on - strand, within Pf1N1B4_3022 at 3399.878 kb on - strand, within Pf1N1B4_3022 at 3399.878 kb on - strand at 3400.152 kb on + strand at 3400.152 kb on + strand at 3400.152 kb on + strand at 3400.177 kb on + strand at 3400.177 kb on + strand at 3400.178 kb on - strand at 3400.178 kb on - strand at 3400.222 kb on + strand at 3400.223 kb on + strand at 3400.223 kb on + strand at 3400.223 kb on + strand at 3400.223 kb on + strand at 3400.223 kb on + strand at 3400.223 kb on + strand at 3400.223 kb on + strand at 3400.223 kb on + strand at 3400.223 kb on + strand at 3400.223 kb on + strand at 3400.223 kb on + strand at 3400.223 kb on + strand at 3400.224 kb on - strand at 3400.224 kb on - strand at 3400.224 kb on - strand at 3400.224 kb on - strand at 3400.224 kb on - strand at 3400.224 kb on - strand at 3400.224 kb on - strand at 3400.224 kb on - strand at 3400.224 kb on - strand at 3400.224 kb on - strand at 3400.227 kb on + strand at 3400.227 kb on + strand at 3400.227 kb on + strand at 3400.227 kb on + strand at 3400.227 kb on + strand at 3400.227 kb on + strand at 3400.228 kb on - strand at 3400.228 kb on - strand at 3400.228 kb on - strand at 3400.320 kb on + strand at 3400.320 kb on + strand at 3400.320 kb on + strand at 3400.320 kb on + strand at 3400.321 kb on - strand at 3400.321 kb on - strand at 3400.321 kb on - strand at 3400.335 kb on + strand at 3400.336 kb on - strand at 3400.336 kb on - strand at 3400.336 kb on - strand at 3400.336 kb on - strand at 3400.341 kb on + strand at 3400.341 kb on + strand at 3400.342 kb on - strand at 3400.342 kb on - strand at 3400.342 kb on - strand at 3400.442 kb on + strand at 3400.443 kb on - strand at 3400.451 kb on + strand at 3400.451 kb on + strand at 3400.451 kb on + strand at 3400.451 kb on + strand at 3400.452 kb on - strand at 3400.468 kb on + strand at 3400.472 kb on + strand at 3400.472 kb on + strand at 3400.472 kb on + strand at 3400.509 kb on + strand at 3400.510 kb on - strand at 3400.510 kb on - strand at 3400.510 kb on - strand at 3400.510 kb on - strand at 3400.514 kb on + strand at 3400.584 kb on + strand at 3400.617 kb on + strand at 3400.617 kb on + strand at 3400.617 kb on + strand at 3400.617 kb on + strand at 3400.617 kb on + strand at 3400.617 kb on + strand at 3400.617 kb on + strand at 3400.617 kb on + strand at 3400.617 kb on + strand at 3400.617 kb on + strand at 3400.618 kb on - strand at 3400.618 kb on - strand at 3400.618 kb on - strand at 3400.618 kb on - strand at 3400.618 kb on - strand at 3400.618 kb on - strand at 3400.619 kb on + strand at 3400.619 kb on + strand at 3400.619 kb on + strand at 3400.619 kb on + strand at 3400.619 kb on + strand at 3400.619 kb on + strand at 3400.619 kb on + strand at 3400.619 kb on + strand at 3400.619 kb on + strand at 3400.619 kb on + strand at 3400.619 kb on + strand at 3400.619 kb on + strand at 3400.619 kb on + strand at 3400.619 kb on + strand at 3400.619 kb on + strand at 3400.620 kb on - strand at 3400.620 kb on - strand at 3400.620 kb on - strand at 3400.645 kb on + strand at 3400.645 kb on + strand at 3400.645 kb on + strand at 3400.645 kb on + strand at 3400.646 kb on - strand at 3400.646 kb on - strand at 3400.666 kb on + strand, within Pf1N1B4_3023 at 3400.667 kb on - strand, within Pf1N1B4_3023 at 3400.667 kb on - strand, within Pf1N1B4_3023 at 3400.667 kb on - strand, within Pf1N1B4_3023 at 3400.832 kb on + strand at 3400.955 kb on + strand at 3400.955 kb on + strand at 3400.955 kb on + strand at 3400.956 kb on - strand at 3400.956 kb on - strand at 3400.956 kb on - strand at 3400.988 kb on + strand at 3400.988 kb on + strand at 3400.988 kb on + strand at 3400.989 kb on - strand at 3400.989 kb on - strand at 3401.003 kb on + strand at 3401.003 kb on + strand at 3401.003 kb on + strand at 3401.004 kb on - strand at 3401.168 kb on + strand, within Pf1N1B4_3024 at 3401.169 kb on - strand, within Pf1N1B4_3024 at 3401.453 kb on + strand, within Pf1N1B4_3024 at 3401.454 kb on - strand, within Pf1N1B4_3024 at 3401.461 kb on - strand, within Pf1N1B4_3024 at 3401.501 kb on + strand, within Pf1N1B4_3024 at 3401.501 kb on + strand, within Pf1N1B4_3024 at 3401.502 kb on - strand, within Pf1N1B4_3024 at 3401.502 kb on - strand, within Pf1N1B4_3024 at 3401.502 kb on - strand at 3401.502 kb on - strand, within Pf1N1B4_3024 at 3401.502 kb on - strand, within Pf1N1B4_3024 at 3401.502 kb on - strand, within Pf1N1B4_3024 at 3401.519 kb on + strand at 3401.520 kb on - strand, within Pf1N1B4_3024 at 3401.520 kb on - strand, within Pf1N1B4_3024 at 3401.558 kb on + strand, within Pf1N1B4_3024 at 3401.558 kb on + strand, within Pf1N1B4_3024 at 3401.558 kb on + strand, within Pf1N1B4_3024 at 3401.559 kb on - strand, within Pf1N1B4_3024 at 3401.559 kb on - strand, within Pf1N1B4_3024 at 3401.559 kb on - strand, within Pf1N1B4_3024 at 3401.577 kb on + strand, within Pf1N1B4_3024 at 3401.577 kb on + strand, within Pf1N1B4_3024 at 3401.577 kb on + strand, within Pf1N1B4_3024 at 3401.578 kb on - strand, within Pf1N1B4_3024 at 3401.578 kb on - strand, within Pf1N1B4_3024 at 3401.584 kb on + strand, within Pf1N1B4_3024 at 3401.585 kb on - strand, within Pf1N1B4_3024 at 3401.728 kb on + strand, within Pf1N1B4_3024 at 3401.729 kb on - strand, within Pf1N1B4_3024 at 3401.778 kb on - strand, within Pf1N1B4_3024 at 3401.787 kb on + strand, within Pf1N1B4_3024 at 3401.787 kb on + strand at 3401.787 kb on + strand, within Pf1N1B4_3024 at 3401.787 kb on + strand, within Pf1N1B4_3024 at 3401.787 kb on + strand, within Pf1N1B4_3024 at 3401.788 kb on - strand, within Pf1N1B4_3024 at 3401.788 kb on - strand, within Pf1N1B4_3024 at 3401.788 kb on - strand, within Pf1N1B4_3024 at 3401.788 kb on - strand, within Pf1N1B4_3024 at 3401.791 kb on + strand, within Pf1N1B4_3024 at 3401.791 kb on + strand, within Pf1N1B4_3024 at 3401.791 kb on + strand, within Pf1N1B4_3024 at 3401.791 kb on + strand, within Pf1N1B4_3024 at 3401.791 kb on + strand, within Pf1N1B4_3024 at 3401.792 kb on - strand, within Pf1N1B4_3024 at 3401.792 kb on - strand, within Pf1N1B4_3024 at 3401.792 kb on - strand, within Pf1N1B4_3024 at 3401.792 kb on - strand, within Pf1N1B4_3024
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 3,399,694 + Pf1N1B4_3022 0.67 +0.0 3,399,694 + Pf1N1B4_3022 0.67 +0.0 3,399,695 - Pf1N1B4_3022 0.67 +0.0 3,399,695 - Pf1N1B4_3022 0.67 +0.0 3,399,695 - Pf1N1B4_3022 0.67 +0.0 3,399,791 + Pf1N1B4_3022 0.73 +0.0 3,399,792 - +0.0 3,399,814 + Pf1N1B4_3022 0.75 -2.3 3,399,877 + Pf1N1B4_3022 0.79 +0.0 3,399,877 + Pf1N1B4_3022 0.79 +0.0 3,399,877 + Pf1N1B4_3022 0.79 +0.0 3,399,877 + Pf1N1B4_3022 0.79 +0.0 3,399,877 + Pf1N1B4_3022 0.79 +0.0 3,399,878 - Pf1N1B4_3022 0.79 -2.0 3,399,878 - Pf1N1B4_3022 0.79 +0.0 3,399,878 - Pf1N1B4_3022 0.79 -1.0 3,399,878 - Pf1N1B4_3022 0.79 +0.0 3,399,878 - Pf1N1B4_3022 0.79 +0.0 3,399,878 - -0.2 3,400,152 + +0.0 3,400,152 + +0.0 3,400,152 + -1.4 3,400,177 + -2.1 3,400,177 + +0.0 3,400,178 - -2.3 3,400,178 - -1.4 3,400,222 + -1.5 3,400,223 + -1.3 3,400,223 + +0.0 3,400,223 + +0.0 3,400,223 + -0.6 3,400,223 + +0.0 3,400,223 + +0.0 3,400,223 + +0.0 3,400,223 + -2.2 3,400,223 + +0.0 3,400,223 + -3.2 3,400,223 + +0.0 3,400,223 + +0.0 3,400,224 - +0.0 3,400,224 - +0.0 3,400,224 - -1.9 3,400,224 - +0.0 3,400,224 - +0.0 3,400,224 - +0.0 3,400,224 - -2.0 3,400,224 - -2.9 3,400,224 - +0.0 3,400,224 - -1.4 3,400,227 + +0.0 3,400,227 + -1.6 3,400,227 + -0.8 3,400,227 + +0.0 3,400,227 + -1.3 3,400,227 + -1.4 3,400,228 - -1.8 3,400,228 - +0.0 3,400,228 - -1.6 3,400,320 + +0.0 3,400,320 + +0.0 3,400,320 + +0.0 3,400,320 + -2.3 3,400,321 - +0.0 3,400,321 - +0.0 3,400,321 - +0.0 3,400,335 + -0.7 3,400,336 - +0.0 3,400,336 - +0.0 3,400,336 - +0.0 3,400,336 - +0.0 3,400,341 + +0.0 3,400,341 + +0.0 3,400,342 - -1.5 3,400,342 - -1.1 3,400,342 - -0.8 3,400,442 + +0.0 3,400,443 - +0.0 3,400,451 + -0.6 3,400,451 + +1.0 3,400,451 + -1.6 3,400,451 + -1.4 3,400,452 - -1.1 3,400,468 + +0.0 3,400,472 + +0.0 3,400,472 + +0.0 3,400,472 + -1.2 3,400,509 + +0.0 3,400,510 - +0.0 3,400,510 - +0.0 3,400,510 - +0.0 3,400,510 - -1.4 3,400,514 + +0.0 3,400,584 + +0.0 3,400,617 + +0.0 3,400,617 + +0.0 3,400,617 + +0.0 3,400,617 + -2.0 3,400,617 + +0.0 3,400,617 + +0.0 3,400,617 + +0.0 3,400,617 + +0.0 3,400,617 + +0.0 3,400,617 + +0.0 3,400,618 - +0.0 3,400,618 - +0.0 3,400,618 - +0.0 3,400,618 - +0.0 3,400,618 - -1.9 3,400,618 - +0.0 3,400,619 + +0.0 3,400,619 + -3.3 3,400,619 + -1.7 3,400,619 + -1.0 3,400,619 + +0.0 3,400,619 + -1.7 3,400,619 + +0.0 3,400,619 + -2.6 3,400,619 + -1.7 3,400,619 + -2.3 3,400,619 + -3.1 3,400,619 + +0.0 3,400,619 + -2.2 3,400,619 + -2.7 3,400,619 + +0.0 3,400,620 - +0.0 3,400,620 - -1.2 3,400,620 - -0.4 3,400,645 + +0.0 3,400,645 + -2.1 3,400,645 + +0.0 3,400,645 + +0.0 3,400,646 - +0.0 3,400,646 - +0.0 3,400,666 + Pf1N1B4_3023 0.17 +0.0 3,400,667 - Pf1N1B4_3023 0.17 +0.0 3,400,667 - Pf1N1B4_3023 0.17 +0.0 3,400,667 - Pf1N1B4_3023 0.17 -2.8 3,400,832 + -1.4 3,400,955 + +0.0 3,400,955 + -1.2 3,400,955 + +0.0 3,400,956 - -0.9 3,400,956 - -1.6 3,400,956 - +0.0 3,400,988 + +0.0 3,400,988 + +0.0 3,400,988 + +0.0 3,400,989 - +0.0 3,400,989 - -1.7 3,401,003 + +0.0 3,401,003 + +0.0 3,401,003 + -1.9 3,401,004 - +0.0 3,401,168 + Pf1N1B4_3024 0.15 -4.6 3,401,169 - Pf1N1B4_3024 0.15 +0.0 3,401,453 + Pf1N1B4_3024 0.35 +0.0 3,401,454 - Pf1N1B4_3024 0.35 +0.0 3,401,461 - Pf1N1B4_3024 0.36 -0.9 3,401,501 + Pf1N1B4_3024 0.38 +0.0 3,401,501 + Pf1N1B4_3024 0.38 +0.0 3,401,502 - Pf1N1B4_3024 0.38 -1.3 3,401,502 - Pf1N1B4_3024 0.38 +0.0 3,401,502 - -0.8 3,401,502 - Pf1N1B4_3024 0.38 +0.0 3,401,502 - Pf1N1B4_3024 0.38 +0.0 3,401,502 - Pf1N1B4_3024 0.38 +0.0 3,401,519 + -3.2 3,401,520 - Pf1N1B4_3024 0.40 -1.7 3,401,520 - Pf1N1B4_3024 0.40 -1.5 3,401,558 + Pf1N1B4_3024 0.42 +0.0 3,401,558 + Pf1N1B4_3024 0.42 -2.2 3,401,558 + Pf1N1B4_3024 0.42 +1.0 3,401,559 - Pf1N1B4_3024 0.42 +0.0 3,401,559 - Pf1N1B4_3024 0.42 -1.5 3,401,559 - Pf1N1B4_3024 0.42 -1.5 3,401,577 + Pf1N1B4_3024 0.44 +0.0 3,401,577 + Pf1N1B4_3024 0.44 +0.0 3,401,577 + Pf1N1B4_3024 0.44 +0.0 3,401,578 - Pf1N1B4_3024 0.44 +0.0 3,401,578 - Pf1N1B4_3024 0.44 +0.0 3,401,584 + Pf1N1B4_3024 0.44 -1.1 3,401,585 - Pf1N1B4_3024 0.44 +0.0 3,401,728 + Pf1N1B4_3024 0.54 +0.0 3,401,729 - Pf1N1B4_3024 0.54 +0.0 3,401,778 - Pf1N1B4_3024 0.58 -2.3 3,401,787 + Pf1N1B4_3024 0.58 +0.0 3,401,787 + -1.9 3,401,787 + Pf1N1B4_3024 0.58 +0.0 3,401,787 + Pf1N1B4_3024 0.58 +0.0 3,401,787 + Pf1N1B4_3024 0.58 +0.0 3,401,788 - Pf1N1B4_3024 0.59 -0.9 3,401,788 - Pf1N1B4_3024 0.59 +0.0 3,401,788 - Pf1N1B4_3024 0.59 -1.0 3,401,788 - Pf1N1B4_3024 0.59 -0.9 3,401,791 + Pf1N1B4_3024 0.59 +0.0 3,401,791 + Pf1N1B4_3024 0.59 +0.0 3,401,791 + Pf1N1B4_3024 0.59 -2.8 3,401,791 + Pf1N1B4_3024 0.59 -1.7 3,401,791 + Pf1N1B4_3024 0.59 -2.1 3,401,792 - Pf1N1B4_3024 0.59 +0.0 3,401,792 - Pf1N1B4_3024 0.59 +0.0 3,401,792 - Pf1N1B4_3024 0.59 +0.0 3,401,792 - Pf1N1B4_3024 0.59 +0.0
Or see this region's nucleotide sequence