Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2970 and Pf1N1B4_2971 are separated by 136 nucleotides Pf1N1B4_2971 and Pf1N1B4_2972 are separated by 67 nucleotides
Pf1N1B4_2970 - FIG00959654: hypothetical protein, at 3,331,772 to 3,332,476
_2970
Pf1N1B4_2971 - hypothetical protein, at 3,332,613 to 3,333,128
_2971
Pf1N1B4_2972 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 3,333,196 to 3,334,257
_2972
Position (kb)
3332
3333
3334 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3331.649 kb on + strand at 3331.649 kb on + strand at 3331.649 kb on + strand at 3331.649 kb on + strand at 3331.649 kb on + strand at 3331.649 kb on + strand at 3331.649 kb on + strand at 3331.649 kb on + strand at 3331.650 kb on - strand at 3331.650 kb on - strand at 3331.691 kb on + strand at 3331.691 kb on + strand at 3331.692 kb on - strand at 3331.709 kb on - strand at 3331.723 kb on + strand at 3331.724 kb on - strand at 3331.765 kb on + strand at 3331.765 kb on + strand at 3331.766 kb on - strand at 3331.766 kb on - strand at 3331.766 kb on - strand at 3331.766 kb on - strand at 3331.766 kb on - strand at 3331.767 kb on + strand at 3331.767 kb on + strand at 3331.768 kb on - strand at 3331.768 kb on - strand at 3331.774 kb on - strand at 3331.792 kb on - strand at 3332.102 kb on + strand, within Pf1N1B4_2970 at 3332.489 kb on - strand at 3332.497 kb on + strand at 3332.498 kb on - strand at 3332.498 kb on - strand at 3332.543 kb on - strand at 3332.543 kb on - strand at 3332.547 kb on + strand at 3332.548 kb on - strand at 3332.548 kb on - strand at 3332.548 kb on - strand at 3332.615 kb on - strand at 3332.699 kb on - strand, within Pf1N1B4_2971 at 3332.699 kb on - strand, within Pf1N1B4_2971 at 3332.699 kb on - strand, within Pf1N1B4_2971 at 3332.786 kb on - strand, within Pf1N1B4_2971 at 3332.813 kb on - strand, within Pf1N1B4_2971 at 3332.813 kb on - strand, within Pf1N1B4_2971 at 3333.018 kb on + strand, within Pf1N1B4_2971 at 3333.018 kb on + strand, within Pf1N1B4_2971 at 3333.018 kb on + strand, within Pf1N1B4_2971 at 3333.018 kb on + strand, within Pf1N1B4_2971 at 3333.019 kb on - strand, within Pf1N1B4_2971 at 3333.019 kb on - strand, within Pf1N1B4_2971 at 3333.067 kb on + strand, within Pf1N1B4_2971 at 3333.067 kb on + strand, within Pf1N1B4_2971 at 3333.068 kb on - strand, within Pf1N1B4_2971 at 3333.091 kb on + strand at 3333.092 kb on - strand at 3333.168 kb on + strand at 3333.197 kb on + strand at 3333.197 kb on + strand at 3333.197 kb on + strand at 3333.198 kb on - strand at 3333.198 kb on - strand at 3333.300 kb on + strand at 3333.304 kb on + strand at 3333.305 kb on - strand, within Pf1N1B4_2972 at 3333.305 kb on - strand, within Pf1N1B4_2972
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 3,331,649 + +0.0 3,331,649 + +0.0 3,331,649 + +0.0 3,331,649 + +0.0 3,331,649 + +0.0 3,331,649 + -1.5 3,331,649 + +0.0 3,331,649 + +0.0 3,331,650 - +0.0 3,331,650 - +0.0 3,331,691 + -2.4 3,331,691 + +0.0 3,331,692 - +0.0 3,331,709 - +0.0 3,331,723 + +0.0 3,331,724 - +0.0 3,331,765 + +0.0 3,331,765 + +0.0 3,331,766 - +0.0 3,331,766 - -0.9 3,331,766 - +0.0 3,331,766 - +0.0 3,331,766 - -2.3 3,331,767 + +0.0 3,331,767 + +0.0 3,331,768 - -2.1 3,331,768 - +0.0 3,331,774 - +0.0 3,331,792 - -0.8 3,332,102 + Pf1N1B4_2970 0.47 -2.5 3,332,489 - -1.9 3,332,497 + +0.0 3,332,498 - +0.0 3,332,498 - +0.0 3,332,543 - +0.0 3,332,543 - +0.0 3,332,547 + -0.6 3,332,548 - -2.2 3,332,548 - -1.9 3,332,548 - -2.1 3,332,615 - +0.0 3,332,699 - Pf1N1B4_2971 0.17 +0.0 3,332,699 - Pf1N1B4_2971 0.17 +0.0 3,332,699 - Pf1N1B4_2971 0.17 -2.5 3,332,786 - Pf1N1B4_2971 0.34 +0.0 3,332,813 - Pf1N1B4_2971 0.39 -3.2 3,332,813 - Pf1N1B4_2971 0.39 +0.0 3,333,018 + Pf1N1B4_2971 0.78 -4.4 3,333,018 + Pf1N1B4_2971 0.78 +0.0 3,333,018 + Pf1N1B4_2971 0.78 +0.0 3,333,018 + Pf1N1B4_2971 0.78 -2.1 3,333,019 - Pf1N1B4_2971 0.79 +0.0 3,333,019 - Pf1N1B4_2971 0.79 +0.0 3,333,067 + Pf1N1B4_2971 0.88 +0.0 3,333,067 + Pf1N1B4_2971 0.88 +0.0 3,333,068 - Pf1N1B4_2971 0.88 +0.0 3,333,091 + +0.0 3,333,092 - +0.9 3,333,168 + +0.0 3,333,197 + +0.0 3,333,197 + -1.7 3,333,197 + -2.2 3,333,198 - +0.0 3,333,198 - +0.0 3,333,300 + +0.0 3,333,304 + -2.7 3,333,305 - Pf1N1B4_2972 0.10 +0.0 3,333,305 - Pf1N1B4_2972 0.10 -1.1
Or see this region's nucleotide sequence