Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2911 and Pf1N1B4_2912 are separated by 296 nucleotides Pf1N1B4_2912 and Pf1N1B4_2913 are separated by 23 nucleotides Pf1N1B4_2913 and Pf1N1B4_2914 are separated by 168 nucleotides
Pf1N1B4_2911 - 1-aminocyclopropane-1-carboxylate deaminase (EC 3.5.99.7), at 3,267,801 to 3,268,814
_2911
Pf1N1B4_2912 - GTP-binding and nucleic acid-binding protein YchF, at 3,269,111 to 3,270,211
_2912
Pf1N1B4_2913 - Peptidyl-tRNA hydrolase (EC 3.1.1.29), at 3,270,235 to 3,270,819
_2913
Pf1N1B4_2914 - LSU ribosomal protein L25p, at 3,270,988 to 3,271,587
_2914
Position (kb)
3269
3270
3271 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3268.113 kb on + strand, within Pf1N1B4_2911 at 3268.113 kb on + strand, within Pf1N1B4_2911 at 3268.114 kb on - strand, within Pf1N1B4_2911 at 3268.114 kb on - strand, within Pf1N1B4_2911 at 3268.114 kb on - strand, within Pf1N1B4_2911 at 3268.154 kb on + strand, within Pf1N1B4_2911 at 3268.243 kb on + strand, within Pf1N1B4_2911 at 3268.281 kb on + strand, within Pf1N1B4_2911 at 3268.281 kb on + strand, within Pf1N1B4_2911 at 3268.282 kb on - strand, within Pf1N1B4_2911 at 3268.304 kb on - strand, within Pf1N1B4_2911 at 3268.318 kb on + strand, within Pf1N1B4_2911 at 3268.318 kb on + strand at 3268.318 kb on + strand, within Pf1N1B4_2911 at 3268.319 kb on - strand, within Pf1N1B4_2911 at 3268.319 kb on - strand, within Pf1N1B4_2911 at 3268.319 kb on - strand, within Pf1N1B4_2911 at 3268.319 kb on - strand, within Pf1N1B4_2911 at 3268.346 kb on + strand, within Pf1N1B4_2911 at 3268.346 kb on + strand, within Pf1N1B4_2911 at 3268.347 kb on - strand, within Pf1N1B4_2911 at 3268.352 kb on - strand, within Pf1N1B4_2911 at 3268.352 kb on - strand, within Pf1N1B4_2911 at 3268.352 kb on - strand at 3268.352 kb on - strand, within Pf1N1B4_2911 at 3268.352 kb on - strand, within Pf1N1B4_2911 at 3268.472 kb on + strand, within Pf1N1B4_2911 at 3268.472 kb on + strand, within Pf1N1B4_2911 at 3268.472 kb on + strand, within Pf1N1B4_2911 at 3268.489 kb on + strand, within Pf1N1B4_2911 at 3268.489 kb on + strand, within Pf1N1B4_2911 at 3268.490 kb on - strand, within Pf1N1B4_2911 at 3268.609 kb on - strand, within Pf1N1B4_2911 at 3268.648 kb on + strand, within Pf1N1B4_2911 at 3268.648 kb on + strand, within Pf1N1B4_2911 at 3268.648 kb on + strand, within Pf1N1B4_2911 at 3268.648 kb on + strand, within Pf1N1B4_2911 at 3268.648 kb on + strand, within Pf1N1B4_2911 at 3268.648 kb on + strand, within Pf1N1B4_2911 at 3268.648 kb on + strand, within Pf1N1B4_2911 at 3268.648 kb on + strand, within Pf1N1B4_2911 at 3268.648 kb on + strand at 3268.648 kb on + strand, within Pf1N1B4_2911 at 3268.648 kb on + strand, within Pf1N1B4_2911 at 3268.649 kb on - strand, within Pf1N1B4_2911 at 3268.649 kb on - strand, within Pf1N1B4_2911 at 3268.649 kb on - strand, within Pf1N1B4_2911 at 3268.649 kb on - strand, within Pf1N1B4_2911 at 3268.649 kb on - strand, within Pf1N1B4_2911 at 3268.649 kb on - strand, within Pf1N1B4_2911 at 3268.649 kb on - strand at 3268.714 kb on + strand at 3268.714 kb on + strand at 3268.715 kb on - strand at 3268.715 kb on - strand at 3268.715 kb on - strand at 3268.715 kb on - strand at 3268.849 kb on + strand at 3268.968 kb on + strand at 3268.969 kb on - strand at 3269.080 kb on + strand at 3269.094 kb on + strand at 3269.094 kb on + strand at 3269.094 kb on + strand at 3269.094 kb on + strand at 3269.095 kb on - strand at 3269.114 kb on + strand at 3269.114 kb on + strand at 3269.114 kb on + strand at 3269.115 kb on - strand at 3269.115 kb on - strand at 3269.115 kb on - strand at 3269.115 kb on - strand at 3269.115 kb on - strand at 3269.115 kb on - strand at 3269.115 kb on - strand at 3269.115 kb on - strand at 3269.115 kb on - strand at 3269.115 kb on - strand at 3269.115 kb on - strand at 3269.115 kb on - strand at 3269.115 kb on - strand at 3269.158 kb on - strand at 3269.158 kb on - strand at 3269.215 kb on - strand at 3269.595 kb on + strand, within Pf1N1B4_2912 at 3269.596 kb on - strand, within Pf1N1B4_2912 at 3269.596 kb on - strand, within Pf1N1B4_2912 at 3269.596 kb on - strand, within Pf1N1B4_2912 at 3269.596 kb on - strand at 3269.596 kb on - strand, within Pf1N1B4_2912 at 3270.230 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 3,268,113 + Pf1N1B4_2911 0.31 -0.0 3,268,113 + Pf1N1B4_2911 0.31 -0.0 3,268,114 - Pf1N1B4_2911 0.31 -1.7 3,268,114 - Pf1N1B4_2911 0.31 -1.3 3,268,114 - Pf1N1B4_2911 0.31 -1.5 3,268,154 + Pf1N1B4_2911 0.35 -1.0 3,268,243 + Pf1N1B4_2911 0.44 -0.0 3,268,281 + Pf1N1B4_2911 0.47 -1.8 3,268,281 + Pf1N1B4_2911 0.47 -0.0 3,268,282 - Pf1N1B4_2911 0.47 -2.1 3,268,304 - Pf1N1B4_2911 0.50 -1.0 3,268,318 + Pf1N1B4_2911 0.51 -0.5 3,268,318 + -1.3 3,268,318 + Pf1N1B4_2911 0.51 -0.0 3,268,319 - Pf1N1B4_2911 0.51 -1.9 3,268,319 - Pf1N1B4_2911 0.51 -1.1 3,268,319 - Pf1N1B4_2911 0.51 -0.0 3,268,319 - Pf1N1B4_2911 0.51 -0.0 3,268,346 + Pf1N1B4_2911 0.54 -1.0 3,268,346 + Pf1N1B4_2911 0.54 -1.0 3,268,347 - Pf1N1B4_2911 0.54 -2.0 3,268,352 - Pf1N1B4_2911 0.54 -0.0 3,268,352 - Pf1N1B4_2911 0.54 -0.0 3,268,352 - -2.4 3,268,352 - Pf1N1B4_2911 0.54 -0.0 3,268,352 - Pf1N1B4_2911 0.54 -1.9 3,268,472 + Pf1N1B4_2911 0.66 -1.9 3,268,472 + Pf1N1B4_2911 0.66 -0.0 3,268,472 + Pf1N1B4_2911 0.66 -0.0 3,268,489 + Pf1N1B4_2911 0.68 -0.0 3,268,489 + Pf1N1B4_2911 0.68 -0.0 3,268,490 - Pf1N1B4_2911 0.68 -0.0 3,268,609 - Pf1N1B4_2911 0.80 -3.2 3,268,648 + Pf1N1B4_2911 0.84 -0.0 3,268,648 + Pf1N1B4_2911 0.84 -1.3 3,268,648 + Pf1N1B4_2911 0.84 -0.0 3,268,648 + Pf1N1B4_2911 0.84 -0.0 3,268,648 + Pf1N1B4_2911 0.84 -0.0 3,268,648 + Pf1N1B4_2911 0.84 -0.0 3,268,648 + Pf1N1B4_2911 0.84 -2.6 3,268,648 + Pf1N1B4_2911 0.84 -0.0 3,268,648 + -0.8 3,268,648 + Pf1N1B4_2911 0.84 -0.0 3,268,648 + Pf1N1B4_2911 0.84 -0.0 3,268,649 - Pf1N1B4_2911 0.84 -1.8 3,268,649 - Pf1N1B4_2911 0.84 -0.0 3,268,649 - Pf1N1B4_2911 0.84 -0.0 3,268,649 - Pf1N1B4_2911 0.84 -0.0 3,268,649 - Pf1N1B4_2911 0.84 -0.0 3,268,649 - Pf1N1B4_2911 0.84 -0.0 3,268,649 - -0.0 3,268,714 + -1.1 3,268,714 + -1.7 3,268,715 - -0.0 3,268,715 - -1.6 3,268,715 - -1.4 3,268,715 - -0.0 3,268,849 + -0.0 3,268,968 + -0.0 3,268,969 - -2.4 3,269,080 + -0.0 3,269,094 + -2.3 3,269,094 + -0.0 3,269,094 + -3.7 3,269,094 + -1.9 3,269,095 - -1.7 3,269,114 + -0.0 3,269,114 + -0.0 3,269,114 + -0.0 3,269,115 - -0.0 3,269,115 - -1.6 3,269,115 - -1.9 3,269,115 - -0.0 3,269,115 - -3.0 3,269,115 - -2.6 3,269,115 - -0.0 3,269,115 - -0.0 3,269,115 - -0.0 3,269,115 - -0.0 3,269,115 - -0.0 3,269,115 - -4.9 3,269,115 - -2.6 3,269,158 - -0.0 3,269,158 - -0.0 3,269,215 - -0.0 3,269,595 + Pf1N1B4_2912 0.44 -0.0 3,269,596 - Pf1N1B4_2912 0.44 -3.6 3,269,596 - Pf1N1B4_2912 0.44 -0.0 3,269,596 - Pf1N1B4_2912 0.44 -3.0 3,269,596 - -1.6 3,269,596 - Pf1N1B4_2912 0.44 -0.0 3,270,230 + -2.6
Or see this region's nucleotide sequence