Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2516 and Pf1N1B4_2517 are separated by 23 nucleotides Pf1N1B4_2517 and Pf1N1B4_2518 are separated by 74 nucleotides Pf1N1B4_2518 and Pf1N1B4_2519 overlap by 10 nucleotides
Pf1N1B4_2516 - TsaD/Kae1/Qri7 protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA, at 2,855,695 to 2,856,720
_2516
Pf1N1B4_2517 - Acyl-phosphate:glycerol-3-phosphate O-acyltransferase PlsY, at 2,856,744 to 2,857,313
_2517
Pf1N1B4_2518 - Dihydroneopterin aldolase (EC 4.1.2.25), at 2,857,388 to 2,857,744
_2518
Pf1N1B4_2519 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3), at 2,857,735 to 2,858,253
_2519
Position (kb)
2856
2857
2858 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2856.741 kb on + strand at 2856.746 kb on - strand at 2856.780 kb on + strand at 2856.780 kb on + strand at 2856.780 kb on + strand at 2856.781 kb on - strand at 2856.834 kb on + strand, within Pf1N1B4_2517 at 2856.919 kb on + strand, within Pf1N1B4_2517 at 2856.919 kb on + strand, within Pf1N1B4_2517 at 2856.919 kb on + strand, within Pf1N1B4_2517 at 2856.920 kb on - strand, within Pf1N1B4_2517 at 2856.920 kb on - strand, within Pf1N1B4_2517 at 2856.967 kb on + strand, within Pf1N1B4_2517 at 2857.027 kb on + strand, within Pf1N1B4_2517 at 2857.083 kb on + strand, within Pf1N1B4_2517 at 2857.083 kb on + strand, within Pf1N1B4_2517 at 2857.083 kb on + strand, within Pf1N1B4_2517 at 2857.095 kb on + strand, within Pf1N1B4_2517 at 2857.096 kb on - strand, within Pf1N1B4_2517 at 2857.316 kb on + strand at 2857.316 kb on + strand at 2857.316 kb on + strand at 2857.320 kb on + strand at 2857.320 kb on + strand at 2857.320 kb on + strand at 2857.320 kb on + strand at 2857.320 kb on + strand at 2857.320 kb on + strand at 2857.320 kb on + strand at 2858.288 kb on + strand at 2858.288 kb on + strand at 2858.313 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 2,856,741 + -0.0 2,856,746 - -1.0 2,856,780 + -0.0 2,856,780 + -1.5 2,856,780 + -2.9 2,856,781 - -0.0 2,856,834 + Pf1N1B4_2517 0.16 -1.8 2,856,919 + Pf1N1B4_2517 0.31 -0.0 2,856,919 + Pf1N1B4_2517 0.31 -0.0 2,856,919 + Pf1N1B4_2517 0.31 -4.0 2,856,920 - Pf1N1B4_2517 0.31 -3.1 2,856,920 - Pf1N1B4_2517 0.31 -0.0 2,856,967 + Pf1N1B4_2517 0.39 -0.0 2,857,027 + Pf1N1B4_2517 0.50 -2.4 2,857,083 + Pf1N1B4_2517 0.59 -0.0 2,857,083 + Pf1N1B4_2517 0.59 -0.0 2,857,083 + Pf1N1B4_2517 0.59 -0.0 2,857,095 + Pf1N1B4_2517 0.62 -0.0 2,857,096 - Pf1N1B4_2517 0.62 -0.0 2,857,316 + -0.0 2,857,316 + -0.0 2,857,316 + -1.8 2,857,320 + -0.0 2,857,320 + -0.0 2,857,320 + -0.0 2,857,320 + -1.8 2,857,320 + -0.0 2,857,320 + -0.0 2,857,320 + -1.6 2,858,288 + -2.3 2,858,288 + -0.8 2,858,313 + -1.8
Or see this region's nucleotide sequence