Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2430

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2429 and Pf1N1B4_2430 are separated by 139 nucleotidesPf1N1B4_2430 and Pf1N1B4_2431 are separated by 75 nucleotides Pf1N1B4_2429 - Carnitine 3-dehydrogenase (EC 1.1.1.108) (from data), at 2,754,168 to 2,755,133 _2429 Pf1N1B4_2430 - FIG004891: hypothetical protein in carnitine cluster, at 2,755,273 to 2,756,160 _2430 Pf1N1B4_2431 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1), at 2,756,236 to 2,757,180 _2431 Position (kb) 2755 2756 2757Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 2754.346 kb on + strand, within Pf1N1B4_2429at 2754.346 kb on + strand, within Pf1N1B4_2429at 2754.346 kb on + strand, within Pf1N1B4_2429at 2754.346 kb on + strand, within Pf1N1B4_2429at 2754.347 kb on - strand, within Pf1N1B4_2429at 2754.347 kb on - strand, within Pf1N1B4_2429at 2754.424 kb on + strand, within Pf1N1B4_2429at 2754.424 kb on + strand, within Pf1N1B4_2429at 2754.424 kb on + strand, within Pf1N1B4_2429at 2754.425 kb on - strand, within Pf1N1B4_2429at 2754.425 kb on - strand, within Pf1N1B4_2429at 2754.425 kb on - strand, within Pf1N1B4_2429at 2754.425 kb on - strand, within Pf1N1B4_2429at 2754.438 kb on - strand, within Pf1N1B4_2429at 2754.577 kb on - strandat 2754.752 kb on - strand, within Pf1N1B4_2429at 2755.035 kb on + strand, within Pf1N1B4_2429at 2755.125 kb on - strandat 2755.144 kb on + strandat 2755.145 kb on - strandat 2755.256 kb on + strandat 2755.256 kb on + strandat 2755.256 kb on + strandat 2755.263 kb on + strandat 2755.263 kb on + strandat 2755.263 kb on + strandat 2755.264 kb on - strandat 2755.264 kb on - strandat 2755.736 kb on + strand, within Pf1N1B4_2430at 2755.736 kb on + strand, within Pf1N1B4_2430at 2755.736 kb on + strand, within Pf1N1B4_2430at 2755.736 kb on + strand, within Pf1N1B4_2430at 2755.736 kb on + strand, within Pf1N1B4_2430at 2755.736 kb on + strand, within Pf1N1B4_2430at 2755.736 kb on + strand, within Pf1N1B4_2430at 2755.736 kb on + strand, within Pf1N1B4_2430at 2755.736 kb on + strand, within Pf1N1B4_2430at 2755.737 kb on - strand, within Pf1N1B4_2430at 2755.737 kb on - strand, within Pf1N1B4_2430at 2755.737 kb on - strand, within Pf1N1B4_2430at 2755.737 kb on - strand, within Pf1N1B4_2430at 2756.186 kb on - strandat 2756.186 kb on - strandat 2756.186 kb on - strandat 2756.237 kb on + strandat 2756.237 kb on + strandat 2756.238 kb on - strandat 2756.238 kb on - strandat 2756.411 kb on + strand, within Pf1N1B4_2431at 2756.411 kb on + strand, within Pf1N1B4_2431at 2756.412 kb on - strand, within Pf1N1B4_2431at 2756.534 kb on + strand, within Pf1N1B4_2431at 2756.534 kb on + strand, within Pf1N1B4_2431at 2756.535 kb on - strand, within Pf1N1B4_2431at 2756.535 kb on - strand, within Pf1N1B4_2431at 2756.535 kb on - strand, within Pf1N1B4_2431at 2756.535 kb on - strand, within Pf1N1B4_2431at 2756.535 kb on - strand, within Pf1N1B4_2431at 2756.739 kb on - strand, within Pf1N1B4_2431at 2756.810 kb on + strand, within Pf1N1B4_2431at 2756.810 kb on + strand, within Pf1N1B4_2431at 2756.811 kb on - strand, within Pf1N1B4_2431at 2757.006 kb on - strand, within Pf1N1B4_2431at 2757.006 kb on - strand, within Pf1N1B4_2431

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
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2,754,346 + Pf1N1B4_2429 0.18 -2.0
2,754,346 + Pf1N1B4_2429 0.18 -0.0
2,754,346 + Pf1N1B4_2429 0.18 -0.0
2,754,346 + Pf1N1B4_2429 0.18 -1.3
2,754,347 - Pf1N1B4_2429 0.19 -2.8
2,754,347 - Pf1N1B4_2429 0.19 -0.0
2,754,424 + Pf1N1B4_2429 0.27 -0.0
2,754,424 + Pf1N1B4_2429 0.27 -1.8
2,754,424 + Pf1N1B4_2429 0.27 -0.0
2,754,425 - Pf1N1B4_2429 0.27 -0.0
2,754,425 - Pf1N1B4_2429 0.27 -2.1
2,754,425 - Pf1N1B4_2429 0.27 -0.0
2,754,425 - Pf1N1B4_2429 0.27 -1.8
2,754,438 - Pf1N1B4_2429 0.28 -1.9
2,754,577 - -0.0
2,754,752 - Pf1N1B4_2429 0.60 -0.0
2,755,035 + Pf1N1B4_2429 0.90 -1.9
2,755,125 - -0.0
2,755,144 + -2.9
2,755,145 - -0.0
2,755,256 + -2.7
2,755,256 + -1.6
2,755,256 + -0.0
2,755,263 + -0.0
2,755,263 + -1.3
2,755,263 + -0.0
2,755,264 - -2.5
2,755,264 - -0.0
2,755,736 + Pf1N1B4_2430 0.52 -2.4
2,755,736 + Pf1N1B4_2430 0.52 -0.0
2,755,736 + Pf1N1B4_2430 0.52 -2.6
2,755,736 + Pf1N1B4_2430 0.52 -3.3
2,755,736 + Pf1N1B4_2430 0.52 -5.0
2,755,736 + Pf1N1B4_2430 0.52 -0.0
2,755,736 + Pf1N1B4_2430 0.52 -0.0
2,755,736 + Pf1N1B4_2430 0.52 -2.0
2,755,736 + Pf1N1B4_2430 0.52 -0.0
2,755,737 - Pf1N1B4_2430 0.52 -2.7
2,755,737 - Pf1N1B4_2430 0.52 -1.8
2,755,737 - Pf1N1B4_2430 0.52 -0.0
2,755,737 - Pf1N1B4_2430 0.52 -1.5
2,756,186 - -0.8
2,756,186 - -0.0
2,756,186 - -0.0
2,756,237 + -0.0
2,756,237 + -0.0
2,756,238 - -2.1
2,756,238 - -0.0
2,756,411 + Pf1N1B4_2431 0.19 -0.0
2,756,411 + Pf1N1B4_2431 0.19 -0.0
2,756,412 - Pf1N1B4_2431 0.19 -1.2
2,756,534 + Pf1N1B4_2431 0.32 -2.9
2,756,534 + Pf1N1B4_2431 0.32 -1.7
2,756,535 - Pf1N1B4_2431 0.32 -0.0
2,756,535 - Pf1N1B4_2431 0.32 -0.0
2,756,535 - Pf1N1B4_2431 0.32 -0.0
2,756,535 - Pf1N1B4_2431 0.32 -0.0
2,756,535 - Pf1N1B4_2431 0.32 -0.0
2,756,739 - Pf1N1B4_2431 0.53 -0.0
2,756,810 + Pf1N1B4_2431 0.61 -1.2
2,756,810 + Pf1N1B4_2431 0.61 -0.0
2,756,811 - Pf1N1B4_2431 0.61 -0.0
2,757,006 - Pf1N1B4_2431 0.81 -0.0
2,757,006 - Pf1N1B4_2431 0.81 -0.0

Or see this region's nucleotide sequence