Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2410 and Pf1N1B4_2411 are separated by 223 nucleotides Pf1N1B4_2411 and Pf1N1B4_2412 are separated by 131 nucleotides
Pf1N1B4_2410 - FIG00956403: hypothetical protein, at 2,728,623 to 2,730,806
_2410
Pf1N1B4_2411 - FIG00953568: hypothetical protein, at 2,731,030 to 2,732,208
_2411
Pf1N1B4_2412 - ATP-dependent 23S rRNA helicase DbpA, at 2,732,340 to 2,733,725
_2412
Position (kb)
2731
2732
2733 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2730.061 kb on + strand, within Pf1N1B4_2410 at 2730.061 kb on + strand, within Pf1N1B4_2410 at 2730.245 kb on - strand, within Pf1N1B4_2410 at 2730.245 kb on - strand, within Pf1N1B4_2410 at 2730.245 kb on - strand, within Pf1N1B4_2410 at 2730.245 kb on - strand, within Pf1N1B4_2410 at 2730.245 kb on - strand, within Pf1N1B4_2410 at 2730.245 kb on - strand, within Pf1N1B4_2410 at 2730.422 kb on - strand, within Pf1N1B4_2410 at 2730.422 kb on - strand, within Pf1N1B4_2410 at 2730.522 kb on - strand, within Pf1N1B4_2410 at 2730.522 kb on - strand, within Pf1N1B4_2410 at 2730.838 kb on + strand at 2730.839 kb on - strand at 2730.851 kb on + strand at 2730.851 kb on + strand at 2730.917 kb on - strand at 2730.917 kb on - strand at 2730.958 kb on - strand at 2730.958 kb on - strand at 2731.017 kb on + strand at 2731.017 kb on + strand at 2731.017 kb on + strand at 2731.017 kb on + strand at 2731.018 kb on - strand at 2731.127 kb on + strand at 2731.128 kb on - strand at 2731.168 kb on + strand, within Pf1N1B4_2411 at 2731.169 kb on - strand, within Pf1N1B4_2411 at 2731.289 kb on + strand, within Pf1N1B4_2411 at 2731.290 kb on - strand, within Pf1N1B4_2411 at 2731.290 kb on - strand at 2731.980 kb on - strand, within Pf1N1B4_2411 at 2732.379 kb on - strand at 2732.434 kb on + strand at 2732.435 kb on - strand at 2732.435 kb on - strand at 2732.435 kb on - strand at 2732.527 kb on - strand, within Pf1N1B4_2412 at 2732.527 kb on - strand, within Pf1N1B4_2412 at 2732.527 kb on - strand, within Pf1N1B4_2412 at 2732.527 kb on - strand, within Pf1N1B4_2412 at 2732.792 kb on + strand, within Pf1N1B4_2412 at 2732.843 kb on + strand at 2732.844 kb on - strand, within Pf1N1B4_2412 at 2732.844 kb on - strand, within Pf1N1B4_2412 at 2732.869 kb on - strand, within Pf1N1B4_2412 at 2733.055 kb on + strand, within Pf1N1B4_2412 at 2733.055 kb on + strand, within Pf1N1B4_2412 at 2733.055 kb on + strand, within Pf1N1B4_2412 at 2733.056 kb on - strand, within Pf1N1B4_2412
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 2,730,061 + Pf1N1B4_2410 0.66 -0.0 2,730,061 + Pf1N1B4_2410 0.66 -0.0 2,730,245 - Pf1N1B4_2410 0.74 -0.8 2,730,245 - Pf1N1B4_2410 0.74 -1.8 2,730,245 - Pf1N1B4_2410 0.74 -0.0 2,730,245 - Pf1N1B4_2410 0.74 -0.0 2,730,245 - Pf1N1B4_2410 0.74 -1.8 2,730,245 - Pf1N1B4_2410 0.74 -0.0 2,730,422 - Pf1N1B4_2410 0.82 -0.0 2,730,422 - Pf1N1B4_2410 0.82 -1.2 2,730,522 - Pf1N1B4_2410 0.87 -0.0 2,730,522 - Pf1N1B4_2410 0.87 -0.0 2,730,838 + -0.0 2,730,839 - -0.0 2,730,851 + -1.3 2,730,851 + -0.0 2,730,917 - -0.0 2,730,917 - +0.7 2,730,958 - -3.7 2,730,958 - -4.1 2,731,017 + -1.5 2,731,017 + -0.0 2,731,017 + -0.0 2,731,017 + -3.0 2,731,018 - -0.0 2,731,127 + -0.0 2,731,128 - -0.0 2,731,168 + Pf1N1B4_2411 0.12 -0.0 2,731,169 - Pf1N1B4_2411 0.12 -3.4 2,731,289 + Pf1N1B4_2411 0.22 -0.0 2,731,290 - Pf1N1B4_2411 0.22 -0.0 2,731,290 - -2.8 2,731,980 - Pf1N1B4_2411 0.81 -0.0 2,732,379 - +0.0 2,732,434 + +0.0 2,732,435 - -1.8 2,732,435 - +0.0 2,732,435 - +0.0 2,732,527 - Pf1N1B4_2412 0.13 -0.5 2,732,527 - Pf1N1B4_2412 0.13 -0.5 2,732,527 - Pf1N1B4_2412 0.13 +0.0 2,732,527 - Pf1N1B4_2412 0.13 +0.0 2,732,792 + Pf1N1B4_2412 0.33 +0.0 2,732,843 + +0.0 2,732,844 - Pf1N1B4_2412 0.36 -3.1 2,732,844 - Pf1N1B4_2412 0.36 +0.0 2,732,869 - Pf1N1B4_2412 0.38 +0.0 2,733,055 + Pf1N1B4_2412 0.52 +0.0 2,733,055 + Pf1N1B4_2412 0.52 +0.0 2,733,055 + Pf1N1B4_2412 0.52 +0.0 2,733,056 - Pf1N1B4_2412 0.52 +0.0
Or see this region's nucleotide sequence