Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2405 and Pf1N1B4_2406 are separated by 45 nucleotides Pf1N1B4_2406 and Pf1N1B4_2407 are separated by 100 nucleotides Pf1N1B4_2407 and Pf1N1B4_2408 are separated by 123 nucleotides Pf1N1B4_2408 and Pf1N1B4_2409 are separated by 259 nucleotides
Pf1N1B4_2405 - Sensory box/GGDEF family protein, at 2,723,860 to 2,725,965
_2405
Pf1N1B4_2406 - COG1451: Predicted metal-dependent hydrolase, at 2,726,011 to 2,726,502
_2406
Pf1N1B4_2407 - FIG00953367: hypothetical protein, at 2,726,603 to 2,726,899
_2407
Pf1N1B4_2408 - ElaA protein, at 2,727,023 to 2,727,475
_2408
Pf1N1B4_2409 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 2,727,735 to 2,728,472
_2409
Position (kb)
2726
2727 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2725.808 kb on + strand at 2725.808 kb on + strand at 2725.808 kb on + strand at 2725.843 kb on + strand at 2725.873 kb on + strand at 2725.873 kb on + strand at 2725.873 kb on + strand at 2725.874 kb on - strand at 2725.874 kb on - strand at 2725.874 kb on - strand at 2725.874 kb on - strand at 2725.874 kb on - strand at 2725.973 kb on - strand at 2725.973 kb on - strand at 2726.026 kb on + strand at 2726.026 kb on + strand at 2726.026 kb on + strand at 2726.026 kb on + strand at 2726.047 kb on - strand at 2726.047 kb on - strand at 2726.152 kb on + strand, within Pf1N1B4_2406 at 2726.152 kb on + strand, within Pf1N1B4_2406 at 2726.152 kb on + strand, within Pf1N1B4_2406 at 2726.152 kb on + strand at 2726.152 kb on + strand, within Pf1N1B4_2406 at 2726.153 kb on - strand, within Pf1N1B4_2406 at 2726.153 kb on - strand, within Pf1N1B4_2406 at 2726.158 kb on + strand, within Pf1N1B4_2406 at 2726.158 kb on + strand, within Pf1N1B4_2406 at 2726.158 kb on + strand, within Pf1N1B4_2406 at 2726.158 kb on + strand, within Pf1N1B4_2406 at 2726.158 kb on + strand, within Pf1N1B4_2406 at 2726.182 kb on + strand, within Pf1N1B4_2406 at 2726.183 kb on - strand, within Pf1N1B4_2406 at 2726.416 kb on + strand, within Pf1N1B4_2406 at 2726.416 kb on + strand at 2726.416 kb on + strand, within Pf1N1B4_2406 at 2726.416 kb on + strand, within Pf1N1B4_2406 at 2726.417 kb on - strand, within Pf1N1B4_2406 at 2726.468 kb on + strand at 2726.469 kb on - strand at 2726.469 kb on - strand at 2726.469 kb on - strand at 2726.469 kb on - strand at 2726.469 kb on - strand at 2726.469 kb on - strand at 2726.469 kb on - strand at 2726.476 kb on + strand at 2726.476 kb on + strand at 2726.476 kb on + strand at 2726.476 kb on + strand at 2726.477 kb on - strand at 2726.493 kb on + strand at 2726.500 kb on + strand at 2726.500 kb on + strand at 2726.500 kb on + strand at 2726.500 kb on + strand at 2726.500 kb on + strand at 2726.500 kb on + strand at 2726.500 kb on + strand at 2726.500 kb on + strand at 2726.500 kb on + strand at 2726.500 kb on + strand at 2726.501 kb on - strand at 2726.501 kb on - strand at 2726.501 kb on - strand at 2726.501 kb on - strand at 2726.586 kb on + strand at 2726.586 kb on + strand at 2726.586 kb on + strand at 2726.587 kb on - strand at 2726.589 kb on + strand at 2726.589 kb on + strand at 2726.589 kb on + strand at 2726.645 kb on + strand, within Pf1N1B4_2407 at 2726.645 kb on + strand, within Pf1N1B4_2407 at 2726.645 kb on + strand, within Pf1N1B4_2407 at 2726.645 kb on + strand, within Pf1N1B4_2407 at 2726.645 kb on + strand, within Pf1N1B4_2407 at 2726.646 kb on - strand, within Pf1N1B4_2407 at 2726.646 kb on - strand, within Pf1N1B4_2407 at 2726.655 kb on - strand, within Pf1N1B4_2407 at 2726.860 kb on + strand, within Pf1N1B4_2407 at 2726.860 kb on + strand, within Pf1N1B4_2407 at 2726.860 kb on + strand, within Pf1N1B4_2407 at 2726.861 kb on - strand, within Pf1N1B4_2407 at 2726.875 kb on + strand at 2726.884 kb on - strand at 2726.902 kb on - strand at 2727.024 kb on + strand at 2727.066 kb on + strand at 2727.103 kb on - strand, within Pf1N1B4_2408 at 2727.103 kb on - strand, within Pf1N1B4_2408 at 2727.106 kb on - strand, within Pf1N1B4_2408 at 2727.106 kb on - strand, within Pf1N1B4_2408 at 2727.136 kb on - strand, within Pf1N1B4_2408 at 2727.136 kb on - strand, within Pf1N1B4_2408 at 2727.136 kb on - strand, within Pf1N1B4_2408 at 2727.136 kb on - strand, within Pf1N1B4_2408 at 2727.136 kb on - strand, within Pf1N1B4_2408 at 2727.136 kb on - strand, within Pf1N1B4_2408 at 2727.188 kb on + strand, within Pf1N1B4_2408 at 2727.188 kb on + strand, within Pf1N1B4_2408 at 2727.189 kb on - strand, within Pf1N1B4_2408 at 2727.189 kb on - strand, within Pf1N1B4_2408 at 2727.189 kb on - strand, within Pf1N1B4_2408 at 2727.264 kb on - strand, within Pf1N1B4_2408 at 2727.264 kb on - strand, within Pf1N1B4_2408 at 2727.288 kb on + strand at 2727.288 kb on + strand, within Pf1N1B4_2408 at 2727.289 kb on - strand, within Pf1N1B4_2408 at 2727.289 kb on - strand, within Pf1N1B4_2408 at 2727.367 kb on - strand, within Pf1N1B4_2408 at 2727.420 kb on + strand, within Pf1N1B4_2408 at 2727.420 kb on + strand, within Pf1N1B4_2408 at 2727.420 kb on + strand, within Pf1N1B4_2408 at 2727.420 kb on + strand, within Pf1N1B4_2408 at 2727.420 kb on + strand, within Pf1N1B4_2408 at 2727.421 kb on - strand, within Pf1N1B4_2408 at 2727.421 kb on - strand, within Pf1N1B4_2408 at 2727.421 kb on - strand, within Pf1N1B4_2408 at 2727.509 kb on + strand at 2727.509 kb on + strand at 2727.509 kb on + strand at 2727.509 kb on + strand at 2727.509 kb on + strand at 2727.510 kb on - strand at 2727.510 kb on - strand at 2727.510 kb on - strand at 2727.604 kb on + strand at 2727.604 kb on + strand at 2727.605 kb on - strand at 2727.605 kb on - strand at 2727.638 kb on + strand at 2727.639 kb on - strand at 2727.643 kb on + strand at 2727.643 kb on + strand at 2727.643 kb on + strand at 2727.643 kb on + strand at 2727.644 kb on - strand at 2727.644 kb on - strand at 2727.644 kb on - strand at 2727.644 kb on - strand at 2727.644 kb on - strand at 2727.644 kb on - strand at 2727.644 kb on - strand at 2727.649 kb on + strand at 2727.650 kb on - strand at 2727.669 kb on + strand at 2727.887 kb on + strand, within Pf1N1B4_2409
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 2,725,808 + -2.0 2,725,808 + -0.0 2,725,808 + -0.0 2,725,843 + -1.5 2,725,873 + -0.0 2,725,873 + -0.0 2,725,873 + -2.4 2,725,874 - -0.0 2,725,874 - -2.4 2,725,874 - -0.0 2,725,874 - -0.0 2,725,874 - -1.1 2,725,973 - -2.2 2,725,973 - -1.4 2,726,026 + -0.0 2,726,026 + -0.0 2,726,026 + -0.0 2,726,026 + -2.0 2,726,047 - -0.4 2,726,047 - -1.8 2,726,152 + Pf1N1B4_2406 0.29 -2.0 2,726,152 + Pf1N1B4_2406 0.29 -0.0 2,726,152 + Pf1N1B4_2406 0.29 -0.0 2,726,152 + -0.0 2,726,152 + Pf1N1B4_2406 0.29 -0.0 2,726,153 - Pf1N1B4_2406 0.29 -0.0 2,726,153 - Pf1N1B4_2406 0.29 -1.8 2,726,158 + Pf1N1B4_2406 0.30 -0.0 2,726,158 + Pf1N1B4_2406 0.30 -0.0 2,726,158 + Pf1N1B4_2406 0.30 -1.4 2,726,158 + Pf1N1B4_2406 0.30 -0.0 2,726,158 + Pf1N1B4_2406 0.30 -0.0 2,726,182 + Pf1N1B4_2406 0.35 -0.0 2,726,183 - Pf1N1B4_2406 0.35 -0.0 2,726,416 + Pf1N1B4_2406 0.82 -1.0 2,726,416 + -1.3 2,726,416 + Pf1N1B4_2406 0.82 -3.4 2,726,416 + Pf1N1B4_2406 0.82 -0.0 2,726,417 - Pf1N1B4_2406 0.83 -0.0 2,726,468 + -0.5 2,726,469 - -1.5 2,726,469 - -0.0 2,726,469 - -0.0 2,726,469 - -2.4 2,726,469 - -1.0 2,726,469 - -1.4 2,726,469 - -0.8 2,726,476 + -0.0 2,726,476 + -0.0 2,726,476 + -0.6 2,726,476 + -2.2 2,726,477 - -0.0 2,726,493 + -0.7 2,726,500 + -2.3 2,726,500 + -0.0 2,726,500 + -0.0 2,726,500 + -0.0 2,726,500 + -0.0 2,726,500 + -1.7 2,726,500 + -1.7 2,726,500 + -0.0 2,726,500 + +0.2 2,726,500 + -0.0 2,726,501 - -1.0 2,726,501 - -1.3 2,726,501 - -1.6 2,726,501 - -1.9 2,726,586 + -0.0 2,726,586 + -0.0 2,726,586 + -0.0 2,726,587 - -0.0 2,726,589 + -0.0 2,726,589 + -3.0 2,726,589 + -0.0 2,726,645 + Pf1N1B4_2407 0.14 -1.0 2,726,645 + Pf1N1B4_2407 0.14 -0.0 2,726,645 + Pf1N1B4_2407 0.14 -0.0 2,726,645 + Pf1N1B4_2407 0.14 -2.9 2,726,645 + Pf1N1B4_2407 0.14 -2.5 2,726,646 - Pf1N1B4_2407 0.14 -0.0 2,726,646 - Pf1N1B4_2407 0.14 -0.0 2,726,655 - Pf1N1B4_2407 0.18 -0.0 2,726,860 + Pf1N1B4_2407 0.87 -2.4 2,726,860 + Pf1N1B4_2407 0.87 -0.0 2,726,860 + Pf1N1B4_2407 0.87 -2.1 2,726,861 - Pf1N1B4_2407 0.87 -0.0 2,726,875 + -0.0 2,726,884 - -0.0 2,726,902 - -0.0 2,727,024 + -0.0 2,727,066 + -0.0 2,727,103 - Pf1N1B4_2408 0.18 -1.8 2,727,103 - Pf1N1B4_2408 0.18 -0.0 2,727,106 - Pf1N1B4_2408 0.18 -0.0 2,727,106 - Pf1N1B4_2408 0.18 -2.3 2,727,136 - Pf1N1B4_2408 0.25 -0.0 2,727,136 - Pf1N1B4_2408 0.25 -0.0 2,727,136 - Pf1N1B4_2408 0.25 -0.0 2,727,136 - Pf1N1B4_2408 0.25 -3.2 2,727,136 - Pf1N1B4_2408 0.25 -0.0 2,727,136 - Pf1N1B4_2408 0.25 -0.0 2,727,188 + Pf1N1B4_2408 0.36 -2.1 2,727,188 + Pf1N1B4_2408 0.36 -1.8 2,727,189 - Pf1N1B4_2408 0.37 -2.6 2,727,189 - Pf1N1B4_2408 0.37 -2.5 2,727,189 - Pf1N1B4_2408 0.37 -1.3 2,727,264 - Pf1N1B4_2408 0.53 -0.0 2,727,264 - Pf1N1B4_2408 0.53 -0.0 2,727,288 + -1.6 2,727,288 + Pf1N1B4_2408 0.58 -5.4 2,727,289 - Pf1N1B4_2408 0.59 -1.7 2,727,289 - Pf1N1B4_2408 0.59 -2.5 2,727,367 - Pf1N1B4_2408 0.76 -1.8 2,727,420 + Pf1N1B4_2408 0.88 -0.0 2,727,420 + Pf1N1B4_2408 0.88 -0.0 2,727,420 + Pf1N1B4_2408 0.88 -0.0 2,727,420 + Pf1N1B4_2408 0.88 -2.4 2,727,420 + Pf1N1B4_2408 0.88 -2.5 2,727,421 - Pf1N1B4_2408 0.88 -0.0 2,727,421 - Pf1N1B4_2408 0.88 -0.0 2,727,421 - Pf1N1B4_2408 0.88 -0.0 2,727,509 + -0.0 2,727,509 + -0.0 2,727,509 + -0.0 2,727,509 + -1.9 2,727,509 + -0.0 2,727,510 - -1.0 2,727,510 - -0.0 2,727,510 - -0.0 2,727,604 + -0.0 2,727,604 + -1.3 2,727,605 - -0.0 2,727,605 - -2.1 2,727,638 + -2.6 2,727,639 - -0.0 2,727,643 + -1.9 2,727,643 + -0.0 2,727,643 + -0.0 2,727,643 + -0.0 2,727,644 - -0.0 2,727,644 - -1.7 2,727,644 - -1.1 2,727,644 - -0.0 2,727,644 - -0.0 2,727,644 - -0.0 2,727,644 - -1.4 2,727,649 + -2.4 2,727,650 - -1.7 2,727,669 + -0.0 2,727,887 + Pf1N1B4_2409 0.21 -2.3
Or see this region's nucleotide sequence