Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_231 and Pf1N1B4_232 are separated by 47 nucleotides Pf1N1B4_232 and Pf1N1B4_233 overlap by 17 nucleotides
Pf1N1B4_231 - Sensor histidine kinase, at 292,071 to 292,964
_231
Pf1N1B4_232 - DNA-binding response regulator, LuxR family, at 293,012 to 293,620
_232
Pf1N1B4_233 - Transcription regulator [contains diacylglycerol kinase catalytic domain], at 293,604 to 294,536
_233
Position (kb) 293
294 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 292.070 kb on + strand at 292.071 kb on - strand at 292.074 kb on + strand at 292.075 kb on - strand at 292.075 kb on - strand at 292.095 kb on - strand at 292.095 kb on - strand at 292.095 kb on - strand at 292.148 kb on + strand at 292.148 kb on + strand at 292.149 kb on - strand at 292.193 kb on - strand, within Pf1N1B4_231 at 292.224 kb on - strand, within Pf1N1B4_231 at 292.561 kb on + strand, within Pf1N1B4_231 at 292.561 kb on + strand at 292.562 kb on - strand, within Pf1N1B4_231 at 292.580 kb on + strand, within Pf1N1B4_231 at 292.580 kb on + strand, within Pf1N1B4_231 at 292.580 kb on + strand, within Pf1N1B4_231 at 292.581 kb on - strand, within Pf1N1B4_231 at 292.682 kb on + strand, within Pf1N1B4_231 at 292.682 kb on + strand, within Pf1N1B4_231 at 292.683 kb on - strand at 292.684 kb on + strand, within Pf1N1B4_231 at 292.684 kb on + strand, within Pf1N1B4_231 at 292.684 kb on + strand, within Pf1N1B4_231 at 292.706 kb on + strand, within Pf1N1B4_231 at 292.849 kb on + strand, within Pf1N1B4_231 at 292.849 kb on + strand, within Pf1N1B4_231 at 292.931 kb on - strand at 292.949 kb on + strand at 292.950 kb on - strand at 292.969 kb on + strand at 292.969 kb on + strand at 292.970 kb on - strand at 292.974 kb on + strand at 292.974 kb on + strand at 292.974 kb on + strand at 292.974 kb on + strand at 292.975 kb on - strand at 292.975 kb on - strand at 292.999 kb on + strand at 293.049 kb on + strand at 293.125 kb on + strand at 293.558 kb on + strand, within Pf1N1B4_232 at 293.559 kb on - strand, within Pf1N1B4_232 at 293.559 kb on - strand, within Pf1N1B4_232 at 293.735 kb on + strand, within Pf1N1B4_233 at 293.736 kb on - strand, within Pf1N1B4_233 at 293.736 kb on - strand at 293.737 kb on + strand at 293.738 kb on - strand at 293.738 kb on - strand, within Pf1N1B4_233 at 293.738 kb on - strand, within Pf1N1B4_233 at 293.738 kb on - strand, within Pf1N1B4_233 at 293.738 kb on - strand, within Pf1N1B4_233 at 293.738 kb on - strand at 293.738 kb on - strand, within Pf1N1B4_233 at 294.046 kb on + strand, within Pf1N1B4_233 at 294.046 kb on + strand, within Pf1N1B4_233 at 294.047 kb on - strand, within Pf1N1B4_233 at 294.087 kb on - strand, within Pf1N1B4_233 at 294.087 kb on - strand, within Pf1N1B4_233 at 294.087 kb on - strand, within Pf1N1B4_233 at 294.264 kb on + strand, within Pf1N1B4_233 at 294.265 kb on - strand at 294.351 kb on - strand, within Pf1N1B4_233 at 294.555 kb on + strand at 294.555 kb on + strand at 294.555 kb on + strand at 294.556 kb on - strand at 294.556 kb on - strand at 294.578 kb on + strand at 294.579 kb on - strand at 294.579 kb on - strand at 294.600 kb on + strand at 294.601 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 292,070 + -0.0 292,071 - -0.0 292,074 + -1.5 292,075 - -0.0 292,075 - -0.0 292,095 - -1.4 292,095 - -2.1 292,095 - -0.0 292,148 + -0.0 292,148 + -1.5 292,149 - -3.4 292,193 - Pf1N1B4_231 0.14 -0.0 292,224 - Pf1N1B4_231 0.17 -0.7 292,561 + Pf1N1B4_231 0.55 -0.0 292,561 + -2.4 292,562 - Pf1N1B4_231 0.55 -0.0 292,580 + Pf1N1B4_231 0.57 -2.0 292,580 + Pf1N1B4_231 0.57 -1.2 292,580 + Pf1N1B4_231 0.57 -0.0 292,581 - Pf1N1B4_231 0.57 -1.6 292,682 + Pf1N1B4_231 0.68 -0.0 292,682 + Pf1N1B4_231 0.68 -0.0 292,683 - -1.6 292,684 + Pf1N1B4_231 0.69 -1.3 292,684 + Pf1N1B4_231 0.69 -0.0 292,684 + Pf1N1B4_231 0.69 -1.0 292,706 + Pf1N1B4_231 0.71 -2.7 292,849 + Pf1N1B4_231 0.87 -1.4 292,849 + Pf1N1B4_231 0.87 -0.0 292,931 - -0.0 292,949 + -0.0 292,950 - -2.2 292,969 + -0.0 292,969 + -1.1 292,970 - -2.5 292,974 + -0.0 292,974 + -2.4 292,974 + -0.0 292,974 + -2.5 292,975 - -0.0 292,975 - -2.4 292,999 + -3.1 293,049 + -0.0 293,125 + -1.4 293,558 + Pf1N1B4_232 0.90 -0.0 293,559 - Pf1N1B4_232 0.90 -4.2 293,559 - Pf1N1B4_232 0.90 -0.0 293,735 + Pf1N1B4_233 0.14 -0.0 293,736 - Pf1N1B4_233 0.14 -0.0 293,736 - -0.0 293,737 + -2.3 293,738 - -1.4 293,738 - Pf1N1B4_233 0.14 -0.0 293,738 - Pf1N1B4_233 0.14 -0.0 293,738 - Pf1N1B4_233 0.14 -1.9 293,738 - Pf1N1B4_233 0.14 -0.0 293,738 - -0.0 293,738 - Pf1N1B4_233 0.14 -0.0 294,046 + Pf1N1B4_233 0.47 -0.0 294,046 + Pf1N1B4_233 0.47 -0.0 294,047 - Pf1N1B4_233 0.47 -0.0 294,087 - Pf1N1B4_233 0.52 -1.5 294,087 - Pf1N1B4_233 0.52 -0.0 294,087 - Pf1N1B4_233 0.52 -0.0 294,264 + Pf1N1B4_233 0.71 -0.0 294,265 - -0.0 294,351 - Pf1N1B4_233 0.80 -2.1 294,555 + -0.0 294,555 + -2.3 294,555 + -2.3 294,556 - -1.6 294,556 - -0.0 294,578 + -0.0 294,579 - -0.0 294,579 - -0.0 294,600 + -0.0 294,601 - -1.7
Or see this region's nucleotide sequence