Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_227 and Pf1N1B4_228 are separated by 74 nucleotides Pf1N1B4_228 and Pf1N1B4_229 are separated by 90 nucleotides Pf1N1B4_229 and Pf1N1B4_230 are separated by 463 nucleotides
Pf1N1B4_227 - FIG074102: hypothetical protein, at 288,479 to 288,763
_227
Pf1N1B4_228 - Flavodoxin reductases (ferredoxin-NADPH reductases) family 1, at 288,838 to 289,641
_228
Pf1N1B4_229 - Chemotaxis protein CheV (EC 2.7.3.-), at 289,732 to 290,667
_229
Pf1N1B4_230 - FIG00953472: hypothetical protein, at 291,131 to 291,601
_230
Position (kb) 289
290
291 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 288.740 kb on + strand at 288.740 kb on + strand at 288.740 kb on + strand at 288.740 kb on + strand at 288.741 kb on - strand at 288.839 kb on + strand at 288.840 kb on - strand at 289.072 kb on - strand, within Pf1N1B4_228 at 289.072 kb on - strand, within Pf1N1B4_228 at 289.083 kb on - strand, within Pf1N1B4_228 at 289.661 kb on - strand at 289.675 kb on - strand at 289.675 kb on - strand at 289.677 kb on - strand at 289.722 kb on - strand at 289.741 kb on + strand at 289.741 kb on + strand at 289.742 kb on - strand at 289.742 kb on - strand at 289.742 kb on - strand at 289.742 kb on - strand at 289.771 kb on + strand at 289.772 kb on - strand at 289.880 kb on + strand, within Pf1N1B4_229 at 289.880 kb on + strand, within Pf1N1B4_229 at 289.880 kb on + strand, within Pf1N1B4_229 at 289.881 kb on - strand, within Pf1N1B4_229 at 289.881 kb on - strand, within Pf1N1B4_229 at 289.881 kb on - strand, within Pf1N1B4_229 at 289.881 kb on - strand, within Pf1N1B4_229 at 290.107 kb on - strand, within Pf1N1B4_229 at 290.107 kb on - strand, within Pf1N1B4_229 at 290.193 kb on - strand, within Pf1N1B4_229 at 290.193 kb on - strand, within Pf1N1B4_229 at 290.273 kb on + strand, within Pf1N1B4_229 at 290.274 kb on - strand, within Pf1N1B4_229 at 290.274 kb on - strand, within Pf1N1B4_229 at 290.378 kb on + strand at 290.392 kb on - strand, within Pf1N1B4_229 at 290.626 kb on + strand at 290.626 kb on + strand at 290.626 kb on + strand at 290.627 kb on - strand at 290.644 kb on + strand at 290.702 kb on - strand at 290.848 kb on + strand at 290.849 kb on + strand at 290.849 kb on + strand at 290.850 kb on - strand at 290.850 kb on - strand at 290.850 kb on - strand at 290.877 kb on - strand at 290.879 kb on + strand at 290.880 kb on - strand at 290.880 kb on - strand at 290.901 kb on - strand at 291.071 kb on + strand at 291.071 kb on + strand at 291.071 kb on + strand at 291.071 kb on + strand at 291.071 kb on + strand at 291.072 kb on - strand at 291.072 kb on - strand at 291.072 kb on - strand at 291.072 kb on - strand at 291.072 kb on - strand at 291.072 kb on - strand at 291.072 kb on - strand at 291.072 kb on - strand at 291.072 kb on - strand at 291.072 kb on - strand at 291.126 kb on + strand at 291.127 kb on - strand at 291.127 kb on - strand at 291.127 kb on - strand at 291.127 kb on - strand at 291.127 kb on - strand at 291.127 kb on - strand at 291.127 kb on - strand at 291.127 kb on - strand at 291.127 kb on - strand at 291.127 kb on - strand at 291.344 kb on - strand, within Pf1N1B4_230 at 291.505 kb on - strand, within Pf1N1B4_230 at 291.505 kb on - strand, within Pf1N1B4_230 at 291.600 kb on - strand at 291.600 kb on - strand at 291.600 kb on - strand at 291.633 kb on + strand at 291.653 kb on - strand at 291.654 kb on + strand at 291.655 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 288,740 + -3.0 288,740 + -1.7 288,740 + -1.7 288,740 + -0.0 288,741 - -0.0 288,839 + -0.0 288,840 - -0.0 289,072 - Pf1N1B4_228 0.29 -0.0 289,072 - Pf1N1B4_228 0.29 -1.7 289,083 - Pf1N1B4_228 0.30 -3.1 289,661 - -1.0 289,675 - -1.5 289,675 - -0.0 289,677 - -1.9 289,722 - -0.0 289,741 + -0.0 289,741 + -0.7 289,742 - -0.0 289,742 - -0.0 289,742 - -0.0 289,742 - -0.0 289,771 + -0.0 289,772 - -0.0 289,880 + Pf1N1B4_229 0.16 -0.0 289,880 + Pf1N1B4_229 0.16 -1.8 289,880 + Pf1N1B4_229 0.16 -0.0 289,881 - Pf1N1B4_229 0.16 -1.9 289,881 - Pf1N1B4_229 0.16 -0.0 289,881 - Pf1N1B4_229 0.16 -2.4 289,881 - Pf1N1B4_229 0.16 -1.0 290,107 - Pf1N1B4_229 0.40 -0.0 290,107 - Pf1N1B4_229 0.40 -0.0 290,193 - Pf1N1B4_229 0.49 -0.0 290,193 - Pf1N1B4_229 0.49 -0.0 290,273 + Pf1N1B4_229 0.58 -0.0 290,274 - Pf1N1B4_229 0.58 -3.1 290,274 - Pf1N1B4_229 0.58 -3.4 290,378 + -0.0 290,392 - Pf1N1B4_229 0.71 -2.6 290,626 + -0.0 290,626 + -0.0 290,626 + -0.4 290,627 - -2.8 290,644 + -1.6 290,702 - -0.0 290,848 + -0.0 290,849 + -0.0 290,849 + -1.7 290,850 - -0.0 290,850 - -0.0 290,850 - -0.0 290,877 - -1.2 290,879 + -0.0 290,880 - -1.0 290,880 - -0.0 290,901 - -4.1 291,071 + -0.0 291,071 + -0.0 291,071 + -0.0 291,071 + -0.0 291,071 + -0.0 291,072 - -2.8 291,072 - -2.4 291,072 - -0.0 291,072 - -1.9 291,072 - -3.6 291,072 - -0.0 291,072 - -0.0 291,072 - -1.0 291,072 - -0.0 291,072 - -0.0 291,126 + -2.0 291,127 - -1.2 291,127 - -1.7 291,127 - -0.0 291,127 - -1.7 291,127 - -0.0 291,127 - -0.9 291,127 - +3.5 291,127 - -1.4 291,127 - -1.4 291,127 - -1.3 291,344 - Pf1N1B4_230 0.45 -2.3 291,505 - Pf1N1B4_230 0.79 -0.0 291,505 - Pf1N1B4_230 0.79 -0.0 291,600 - -2.2 291,600 - -0.0 291,600 - -0.0 291,633 + -0.0 291,653 - -0.0 291,654 + -2.8 291,655 - -0.0
Or see this region's nucleotide sequence