Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2095 and Pf1N1B4_2096 overlap by 1 nucleotides Pf1N1B4_2096 and Pf1N1B4_2097 overlap by 4 nucleotides
Pf1N1B4_2095 - IcmF-related protein, at 2,400,094 to 2,403,618
_2095
Pf1N1B4_2096 - Protein serine/threonine phosphatase PrpC, regulation of stationary phase, at 2,403,618 to 2,404,346
_2096
Pf1N1B4_2097 - Serine/threonine protein kinase (EC 2.7.11.1), at 2,404,343 to 2,405,359
_2097
Position (kb)
2403
2404
2405 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2402.858 kb on + strand, within Pf1N1B4_2095 at 2402.858 kb on + strand, within Pf1N1B4_2095 at 2402.911 kb on + strand, within Pf1N1B4_2095 at 2402.911 kb on + strand, within Pf1N1B4_2095 at 2402.918 kb on - strand, within Pf1N1B4_2095 at 2402.947 kb on + strand, within Pf1N1B4_2095 at 2402.948 kb on - strand, within Pf1N1B4_2095 at 2402.956 kb on + strand, within Pf1N1B4_2095 at 2402.957 kb on - strand, within Pf1N1B4_2095 at 2402.957 kb on - strand, within Pf1N1B4_2095 at 2403.076 kb on + strand, within Pf1N1B4_2095 at 2403.076 kb on + strand, within Pf1N1B4_2095 at 2403.076 kb on + strand, within Pf1N1B4_2095 at 2403.076 kb on + strand, within Pf1N1B4_2095 at 2403.076 kb on + strand, within Pf1N1B4_2095 at 2403.076 kb on + strand, within Pf1N1B4_2095 at 2403.076 kb on + strand, within Pf1N1B4_2095 at 2403.076 kb on + strand, within Pf1N1B4_2095 at 2403.076 kb on + strand, within Pf1N1B4_2095 at 2403.077 kb on - strand, within Pf1N1B4_2095 at 2403.077 kb on - strand, within Pf1N1B4_2095 at 2403.077 kb on - strand, within Pf1N1B4_2095 at 2403.077 kb on - strand, within Pf1N1B4_2095 at 2403.077 kb on - strand, within Pf1N1B4_2095 at 2403.077 kb on - strand, within Pf1N1B4_2095 at 2403.077 kb on - strand, within Pf1N1B4_2095 at 2403.077 kb on - strand, within Pf1N1B4_2095 at 2403.077 kb on - strand, within Pf1N1B4_2095 at 2403.077 kb on - strand, within Pf1N1B4_2095 at 2403.077 kb on - strand, within Pf1N1B4_2095 at 2403.077 kb on - strand, within Pf1N1B4_2095 at 2403.160 kb on + strand, within Pf1N1B4_2095 at 2403.160 kb on + strand, within Pf1N1B4_2095 at 2403.332 kb on + strand at 2403.538 kb on + strand at 2403.538 kb on + strand at 2403.538 kb on + strand at 2403.539 kb on - strand at 2403.539 kb on - strand at 2403.539 kb on - strand at 2403.539 kb on - strand at 2403.539 kb on - strand at 2403.539 kb on - strand at 2403.539 kb on - strand at 2403.591 kb on + strand at 2403.591 kb on + strand at 2403.592 kb on - strand at 2403.592 kb on - strand at 2403.601 kb on - strand at 2403.990 kb on + strand, within Pf1N1B4_2096 at 2403.991 kb on - strand, within Pf1N1B4_2096 at 2404.014 kb on + strand, within Pf1N1B4_2096 at 2404.014 kb on + strand, within Pf1N1B4_2096 at 2404.015 kb on - strand, within Pf1N1B4_2096 at 2404.210 kb on - strand, within Pf1N1B4_2096 at 2404.210 kb on - strand, within Pf1N1B4_2096 at 2404.409 kb on + strand at 2404.409 kb on + strand at 2404.409 kb on + strand at 2404.409 kb on + strand at 2404.410 kb on - strand at 2404.610 kb on + strand, within Pf1N1B4_2097 at 2404.610 kb on + strand, within Pf1N1B4_2097 at 2404.610 kb on + strand, within Pf1N1B4_2097 at 2404.611 kb on - strand, within Pf1N1B4_2097 at 2404.611 kb on - strand, within Pf1N1B4_2097 at 2404.611 kb on - strand, within Pf1N1B4_2097 at 2404.611 kb on - strand, within Pf1N1B4_2097 at 2404.627 kb on - strand, within Pf1N1B4_2097 at 2404.627 kb on - strand, within Pf1N1B4_2097 at 2404.771 kb on + strand, within Pf1N1B4_2097 at 2404.884 kb on - strand, within Pf1N1B4_2097 at 2405.105 kb on + strand, within Pf1N1B4_2097 at 2405.106 kb on - strand, within Pf1N1B4_2097 at 2405.126 kb on + strand, within Pf1N1B4_2097 at 2405.127 kb on - strand, within Pf1N1B4_2097 at 2405.127 kb on - strand, within Pf1N1B4_2097 at 2405.127 kb on - strand, within Pf1N1B4_2097 at 2405.224 kb on - strand, within Pf1N1B4_2097
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 2,402,858 + Pf1N1B4_2095 0.78 -1.5 2,402,858 + Pf1N1B4_2095 0.78 -3.0 2,402,911 + Pf1N1B4_2095 0.80 +0.0 2,402,911 + Pf1N1B4_2095 0.80 +0.0 2,402,918 - Pf1N1B4_2095 0.80 +0.0 2,402,947 + Pf1N1B4_2095 0.81 -1.6 2,402,948 - Pf1N1B4_2095 0.81 -1.3 2,402,956 + Pf1N1B4_2095 0.81 +0.0 2,402,957 - Pf1N1B4_2095 0.81 +0.0 2,402,957 - Pf1N1B4_2095 0.81 -2.0 2,403,076 + Pf1N1B4_2095 0.85 +0.0 2,403,076 + Pf1N1B4_2095 0.85 -1.0 2,403,076 + Pf1N1B4_2095 0.85 -1.0 2,403,076 + Pf1N1B4_2095 0.85 -1.6 2,403,076 + Pf1N1B4_2095 0.85 +0.0 2,403,076 + Pf1N1B4_2095 0.85 +0.0 2,403,076 + Pf1N1B4_2095 0.85 -1.1 2,403,076 + Pf1N1B4_2095 0.85 +0.0 2,403,076 + Pf1N1B4_2095 0.85 +0.0 2,403,077 - Pf1N1B4_2095 0.85 -2.8 2,403,077 - Pf1N1B4_2095 0.85 +0.0 2,403,077 - Pf1N1B4_2095 0.85 +0.0 2,403,077 - Pf1N1B4_2095 0.85 +0.0 2,403,077 - Pf1N1B4_2095 0.85 +0.0 2,403,077 - Pf1N1B4_2095 0.85 +0.0 2,403,077 - Pf1N1B4_2095 0.85 -1.6 2,403,077 - Pf1N1B4_2095 0.85 +0.0 2,403,077 - Pf1N1B4_2095 0.85 +0.0 2,403,077 - Pf1N1B4_2095 0.85 -2.6 2,403,077 - Pf1N1B4_2095 0.85 -1.4 2,403,077 - Pf1N1B4_2095 0.85 -0.9 2,403,160 + Pf1N1B4_2095 0.87 +0.0 2,403,160 + Pf1N1B4_2095 0.87 -1.5 2,403,332 + +0.0 2,403,538 + +0.0 2,403,538 + +0.0 2,403,538 + -2.7 2,403,539 - +0.0 2,403,539 - +0.0 2,403,539 - +0.0 2,403,539 - -1.8 2,403,539 - +0.0 2,403,539 - +0.0 2,403,539 - +0.0 2,403,591 + +0.0 2,403,591 + +0.0 2,403,592 - +0.0 2,403,592 - +0.0 2,403,601 - +0.0 2,403,990 + Pf1N1B4_2096 0.51 -2.9 2,403,991 - Pf1N1B4_2096 0.51 +0.0 2,404,014 + Pf1N1B4_2096 0.54 +0.0 2,404,014 + Pf1N1B4_2096 0.54 +0.0 2,404,015 - Pf1N1B4_2096 0.54 -2.6 2,404,210 - Pf1N1B4_2096 0.81 +0.0 2,404,210 - Pf1N1B4_2096 0.81 -1.7 2,404,409 + +0.0 2,404,409 + +0.0 2,404,409 + +0.0 2,404,409 + +0.0 2,404,410 - -0.3 2,404,610 + Pf1N1B4_2097 0.26 +0.0 2,404,610 + Pf1N1B4_2097 0.26 -1.0 2,404,610 + Pf1N1B4_2097 0.26 +0.0 2,404,611 - Pf1N1B4_2097 0.26 +0.0 2,404,611 - Pf1N1B4_2097 0.26 +0.0 2,404,611 - Pf1N1B4_2097 0.26 -2.0 2,404,611 - Pf1N1B4_2097 0.26 -4.3 2,404,627 - Pf1N1B4_2097 0.28 +0.0 2,404,627 - Pf1N1B4_2097 0.28 +0.0 2,404,771 + Pf1N1B4_2097 0.42 +0.0 2,404,884 - Pf1N1B4_2097 0.53 +0.0 2,405,105 + Pf1N1B4_2097 0.75 -1.6 2,405,106 - Pf1N1B4_2097 0.75 +0.0 2,405,126 + Pf1N1B4_2097 0.77 -2.1 2,405,127 - Pf1N1B4_2097 0.77 -3.2 2,405,127 - Pf1N1B4_2097 0.77 +0.0 2,405,127 - Pf1N1B4_2097 0.77 +0.0 2,405,224 - Pf1N1B4_2097 0.87 -2.6
Or see this region's nucleotide sequence