Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1674 and Pf1N1B4_1675 are separated by 124 nucleotides Pf1N1B4_1675 and Pf1N1B4_1676 are separated by 250 nucleotides
Pf1N1B4_1674 - Putative formate dehydrogenase oxidoreductase protein, at 1,917,101 to 1,919,449
_1674
Pf1N1B4_1675 - Transcriptional regulator, at 1,919,574 to 1,920,494
_1675
Pf1N1B4_1676 - Inner membrane component of tripartite multidrug resistance system, at 1,920,745 to 1,922,283
_1676
Position (kb)
1919
1920
1921 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1918.634 kb on - strand, within Pf1N1B4_1674 at 1918.674 kb on + strand, within Pf1N1B4_1674 at 1918.859 kb on + strand, within Pf1N1B4_1674 at 1918.860 kb on - strand, within Pf1N1B4_1674 at 1918.943 kb on + strand, within Pf1N1B4_1674 at 1918.944 kb on - strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.102 kb on + strand at 1919.102 kb on + strand, within Pf1N1B4_1674 at 1919.102 kb on + strand, within Pf1N1B4_1674 at 1919.103 kb on - strand, within Pf1N1B4_1674 at 1919.103 kb on - strand, within Pf1N1B4_1674 at 1919.103 kb on - strand, within Pf1N1B4_1674 at 1919.117 kb on + strand, within Pf1N1B4_1674 at 1919.117 kb on + strand at 1919.118 kb on - strand, within Pf1N1B4_1674 at 1919.118 kb on - strand, within Pf1N1B4_1674 at 1919.118 kb on - strand, within Pf1N1B4_1674 at 1919.315 kb on + strand at 1919.315 kb on + strand at 1919.315 kb on + strand at 1919.316 kb on - strand at 1919.316 kb on - strand at 1919.316 kb on - strand at 1919.318 kb on + strand at 1919.371 kb on + strand at 1919.371 kb on + strand at 1919.372 kb on - strand at 1919.505 kb on + strand at 1919.505 kb on + strand at 1919.505 kb on + strand at 1919.911 kb on + strand, within Pf1N1B4_1675 at 1919.911 kb on + strand, within Pf1N1B4_1675 at 1919.911 kb on + strand, within Pf1N1B4_1675 at 1919.912 kb on - strand, within Pf1N1B4_1675 at 1919.912 kb on - strand, within Pf1N1B4_1675 at 1919.912 kb on - strand, within Pf1N1B4_1675 at 1919.912 kb on - strand, within Pf1N1B4_1675 at 1920.539 kb on + strand at 1920.539 kb on + strand at 1920.539 kb on + strand at 1920.540 kb on - strand at 1920.551 kb on + strand at 1920.663 kb on + strand at 1920.663 kb on + strand at 1920.663 kb on + strand at 1920.663 kb on + strand at 1920.663 kb on + strand at 1920.663 kb on + strand at 1920.663 kb on + strand at 1920.663 kb on + strand at 1920.663 kb on + strand at 1920.663 kb on + strand at 1920.663 kb on + strand at 1920.664 kb on - strand at 1920.664 kb on - strand at 1920.664 kb on - strand at 1920.664 kb on - strand at 1920.664 kb on - strand at 1920.664 kb on - strand at 1920.665 kb on + strand at 1920.665 kb on + strand at 1920.666 kb on - strand at 1920.666 kb on - strand at 1920.666 kb on - strand at 1920.666 kb on - strand at 1920.676 kb on + strand at 1920.708 kb on - strand at 1920.743 kb on + strand at 1920.852 kb on - strand at 1920.964 kb on + strand, within Pf1N1B4_1676 at 1920.964 kb on + strand, within Pf1N1B4_1676 at 1920.965 kb on - strand, within Pf1N1B4_1676 at 1920.965 kb on - strand at 1920.965 kb on - strand, within Pf1N1B4_1676 at 1921.042 kb on + strand, within Pf1N1B4_1676 at 1921.096 kb on + strand, within Pf1N1B4_1676 at 1921.097 kb on - strand, within Pf1N1B4_1676 at 1921.204 kb on + strand, within Pf1N1B4_1676 at 1921.204 kb on + strand, within Pf1N1B4_1676 at 1921.205 kb on - strand, within Pf1N1B4_1676 at 1921.205 kb on - strand, within Pf1N1B4_1676 at 1921.282 kb on + strand, within Pf1N1B4_1676 at 1921.282 kb on + strand, within Pf1N1B4_1676 at 1921.282 kb on + strand, within Pf1N1B4_1676 at 1921.283 kb on - strand, within Pf1N1B4_1676
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 1,918,634 - Pf1N1B4_1674 0.65 -0.0 1,918,674 + Pf1N1B4_1674 0.67 -1.1 1,918,859 + Pf1N1B4_1674 0.75 -0.0 1,918,860 - Pf1N1B4_1674 0.75 -0.0 1,918,943 + Pf1N1B4_1674 0.78 -1.1 1,918,944 - Pf1N1B4_1674 0.78 -1.0 1,919,028 + Pf1N1B4_1674 0.82 -0.0 1,919,028 + Pf1N1B4_1674 0.82 -0.7 1,919,028 + Pf1N1B4_1674 0.82 -0.0 1,919,028 + Pf1N1B4_1674 0.82 -0.0 1,919,028 + Pf1N1B4_1674 0.82 -3.5 1,919,028 + Pf1N1B4_1674 0.82 -0.0 1,919,028 + Pf1N1B4_1674 0.82 -0.0 1,919,028 + Pf1N1B4_1674 0.82 -2.4 1,919,028 + Pf1N1B4_1674 0.82 -0.0 1,919,028 + Pf1N1B4_1674 0.82 -0.0 1,919,028 + Pf1N1B4_1674 0.82 -0.0 1,919,029 - Pf1N1B4_1674 0.82 -0.0 1,919,029 - Pf1N1B4_1674 0.82 -0.0 1,919,029 - Pf1N1B4_1674 0.82 -3.4 1,919,029 - Pf1N1B4_1674 0.82 -0.0 1,919,029 - Pf1N1B4_1674 0.82 -0.2 1,919,029 - Pf1N1B4_1674 0.82 -0.9 1,919,029 - Pf1N1B4_1674 0.82 -0.0 1,919,029 - Pf1N1B4_1674 0.82 -0.0 1,919,029 - Pf1N1B4_1674 0.82 -0.0 1,919,029 - Pf1N1B4_1674 0.82 -0.0 1,919,029 - Pf1N1B4_1674 0.82 -3.6 1,919,029 - Pf1N1B4_1674 0.82 -2.4 1,919,102 + -0.0 1,919,102 + Pf1N1B4_1674 0.85 -0.0 1,919,102 + Pf1N1B4_1674 0.85 -0.0 1,919,103 - Pf1N1B4_1674 0.85 -0.0 1,919,103 - Pf1N1B4_1674 0.85 -1.0 1,919,103 - Pf1N1B4_1674 0.85 -1.9 1,919,117 + Pf1N1B4_1674 0.86 -0.0 1,919,117 + -2.2 1,919,118 - Pf1N1B4_1674 0.86 -0.7 1,919,118 - Pf1N1B4_1674 0.86 -0.0 1,919,118 - Pf1N1B4_1674 0.86 -3.7 1,919,315 + -1.1 1,919,315 + -0.9 1,919,315 + -0.0 1,919,316 - -2.0 1,919,316 - -2.4 1,919,316 - -1.4 1,919,318 + -0.0 1,919,371 + -0.0 1,919,371 + -0.0 1,919,372 - -0.0 1,919,505 + -0.0 1,919,505 + -0.0 1,919,505 + -0.0 1,919,911 + Pf1N1B4_1675 0.37 -0.0 1,919,911 + Pf1N1B4_1675 0.37 -0.0 1,919,911 + Pf1N1B4_1675 0.37 -0.0 1,919,912 - Pf1N1B4_1675 0.37 -0.0 1,919,912 - Pf1N1B4_1675 0.37 -2.4 1,919,912 - Pf1N1B4_1675 0.37 -2.8 1,919,912 - Pf1N1B4_1675 0.37 -0.0 1,920,539 + -0.0 1,920,539 + -0.0 1,920,539 + -0.0 1,920,540 - -0.0 1,920,551 + -0.0 1,920,663 + -1.5 1,920,663 + -0.0 1,920,663 + -0.0 1,920,663 + -0.0 1,920,663 + -1.9 1,920,663 + -0.0 1,920,663 + -0.9 1,920,663 + -0.0 1,920,663 + -0.0 1,920,663 + -0.0 1,920,663 + -0.0 1,920,664 - -0.0 1,920,664 - -0.9 1,920,664 - -0.0 1,920,664 - -0.0 1,920,664 - -0.0 1,920,664 - -0.0 1,920,665 + -1.1 1,920,665 + -1.1 1,920,666 - -0.0 1,920,666 - -0.8 1,920,666 - -0.0 1,920,666 - -0.0 1,920,676 + -0.0 1,920,708 - -0.0 1,920,743 + -1.7 1,920,852 - -0.0 1,920,964 + Pf1N1B4_1676 0.14 -0.0 1,920,964 + Pf1N1B4_1676 0.14 -0.0 1,920,965 - Pf1N1B4_1676 0.14 -0.0 1,920,965 - -1.8 1,920,965 - Pf1N1B4_1676 0.14 -0.0 1,921,042 + Pf1N1B4_1676 0.19 -0.0 1,921,096 + Pf1N1B4_1676 0.23 -1.6 1,921,097 - Pf1N1B4_1676 0.23 -1.5 1,921,204 + Pf1N1B4_1676 0.30 -0.0 1,921,204 + Pf1N1B4_1676 0.30 -0.0 1,921,205 - Pf1N1B4_1676 0.30 -3.0 1,921,205 - Pf1N1B4_1676 0.30 -0.0 1,921,282 + Pf1N1B4_1676 0.35 -0.0 1,921,282 + Pf1N1B4_1676 0.35 -0.0 1,921,282 + Pf1N1B4_1676 0.35 -0.0 1,921,283 - Pf1N1B4_1676 0.35 -0.0
Or see this region's nucleotide sequence