Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1525 and Pf1N1B4_1526 are separated by 35 nucleotides Pf1N1B4_1526 and Pf1N1B4_1527 are separated by 369 nucleotides
Pf1N1B4_1525 - hypothetical protein, at 1,757,191 to 1,757,448
_1525
Pf1N1B4_1526 - hypothetical protein, at 1,757,484 to 1,757,612
_1526
Pf1N1B4_1527 - Queuosine Biosynthesis QueC ATPase, at 1,757,982 to 1,758,671
_1527
Position (kb)
1757
1758
1759 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1757.049 kb on - strand at 1757.049 kb on - strand at 1757.056 kb on + strand at 1757.057 kb on - strand at 1757.057 kb on - strand at 1757.057 kb on - strand at 1757.057 kb on - strand at 1757.057 kb on - strand at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.237 kb on - strand, within Pf1N1B4_1525 at 1757.237 kb on - strand, within Pf1N1B4_1525 at 1757.237 kb on - strand, within Pf1N1B4_1525 at 1757.237 kb on - strand, within Pf1N1B4_1525 at 1757.237 kb on - strand, within Pf1N1B4_1525 at 1757.240 kb on + strand, within Pf1N1B4_1525 at 1757.241 kb on - strand, within Pf1N1B4_1525 at 1757.368 kb on + strand, within Pf1N1B4_1525 at 1757.369 kb on - strand, within Pf1N1B4_1525 at 1757.369 kb on - strand, within Pf1N1B4_1525 at 1757.369 kb on - strand, within Pf1N1B4_1525 at 1757.369 kb on - strand, within Pf1N1B4_1525 at 1757.396 kb on + strand, within Pf1N1B4_1525 at 1757.396 kb on + strand, within Pf1N1B4_1525 at 1757.396 kb on + strand, within Pf1N1B4_1525 at 1757.396 kb on + strand, within Pf1N1B4_1525 at 1757.397 kb on - strand, within Pf1N1B4_1525 at 1757.397 kb on - strand, within Pf1N1B4_1525 at 1757.397 kb on - strand, within Pf1N1B4_1525 at 1757.397 kb on - strand, within Pf1N1B4_1525 at 1757.397 kb on - strand, within Pf1N1B4_1525 at 1757.432 kb on + strand at 1757.432 kb on + strand at 1757.432 kb on + strand at 1757.432 kb on + strand at 1757.433 kb on - strand at 1757.610 kb on + strand at 1757.610 kb on + strand at 1757.610 kb on + strand at 1757.636 kb on - strand at 1757.636 kb on - strand at 1757.636 kb on - strand at 1757.636 kb on - strand at 1757.818 kb on + strand at 1757.818 kb on + strand at 1757.820 kb on + strand at 1757.820 kb on + strand at 1757.821 kb on - strand at 1757.921 kb on + strand at 1757.922 kb on - strand at 1757.988 kb on - strand at 1758.312 kb on + strand, within Pf1N1B4_1527 at 1758.313 kb on - strand, within Pf1N1B4_1527 at 1758.440 kb on + strand, within Pf1N1B4_1527 at 1758.507 kb on + strand, within Pf1N1B4_1527 at 1758.507 kb on + strand, within Pf1N1B4_1527 at 1758.524 kb on - strand, within Pf1N1B4_1527 at 1758.536 kb on + strand, within Pf1N1B4_1527 at 1758.557 kb on + strand, within Pf1N1B4_1527 at 1758.557 kb on + strand, within Pf1N1B4_1527 at 1758.613 kb on + strand at 1758.697 kb on + strand at 1758.826 kb on - strand at 1758.840 kb on - strand at 1758.965 kb on + strand at 1759.044 kb on + strand at 1759.067 kb on + strand at 1759.068 kb on - strand at 1759.068 kb on - strand at 1759.138 kb on + strand at 1759.160 kb on - strand at 1759.212 kb on - strand at 1759.292 kb on + strand at 1759.292 kb on + strand at 1759.292 kb on + strand at 1759.292 kb on + strand at 1759.292 kb on + strand at 1759.342 kb on - strand at 1759.346 kb on - strand at 1759.346 kb on - strand at 1759.514 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B remove 1,757,049 - -1.6 1,757,049 - -0.0 1,757,056 + -1.4 1,757,057 - -0.0 1,757,057 - -0.0 1,757,057 - -0.0 1,757,057 - -0.0 1,757,057 - -0.0 1,757,236 + Pf1N1B4_1525 0.17 -0.0 1,757,236 + Pf1N1B4_1525 0.17 -0.8 1,757,236 + Pf1N1B4_1525 0.17 -0.0 1,757,236 + Pf1N1B4_1525 0.17 -0.0 1,757,236 + Pf1N1B4_1525 0.17 -0.0 1,757,236 + Pf1N1B4_1525 0.17 -0.0 1,757,236 + Pf1N1B4_1525 0.17 -0.0 1,757,236 + Pf1N1B4_1525 0.17 -0.0 1,757,237 - Pf1N1B4_1525 0.18 -0.9 1,757,237 - Pf1N1B4_1525 0.18 -0.0 1,757,237 - Pf1N1B4_1525 0.18 -0.0 1,757,237 - Pf1N1B4_1525 0.18 -0.0 1,757,237 - Pf1N1B4_1525 0.18 -0.9 1,757,240 + Pf1N1B4_1525 0.19 -0.0 1,757,241 - Pf1N1B4_1525 0.19 -0.0 1,757,368 + Pf1N1B4_1525 0.69 -1.8 1,757,369 - Pf1N1B4_1525 0.69 -0.0 1,757,369 - Pf1N1B4_1525 0.69 -0.0 1,757,369 - Pf1N1B4_1525 0.69 -0.0 1,757,369 - Pf1N1B4_1525 0.69 -2.5 1,757,396 + Pf1N1B4_1525 0.79 -0.0 1,757,396 + Pf1N1B4_1525 0.79 -0.0 1,757,396 + Pf1N1B4_1525 0.79 -0.0 1,757,396 + Pf1N1B4_1525 0.79 -0.0 1,757,397 - Pf1N1B4_1525 0.80 -2.0 1,757,397 - Pf1N1B4_1525 0.80 -0.0 1,757,397 - Pf1N1B4_1525 0.80 +1.0 1,757,397 - Pf1N1B4_1525 0.80 -2.0 1,757,397 - Pf1N1B4_1525 0.80 -0.8 1,757,432 + -0.0 1,757,432 + -0.0 1,757,432 + -2.3 1,757,432 + -0.0 1,757,433 - -3.4 1,757,610 + -0.0 1,757,610 + -0.0 1,757,610 + -1.4 1,757,636 - -0.0 1,757,636 - -0.0 1,757,636 - -1.9 1,757,636 - -1.3 1,757,818 + -1.6 1,757,818 + -0.0 1,757,820 + -0.0 1,757,820 + -0.0 1,757,821 - -2.8 1,757,921 + -0.0 1,757,922 - -0.0 1,757,988 - -0.0 1,758,312 + Pf1N1B4_1527 0.48 -0.0 1,758,313 - Pf1N1B4_1527 0.48 -1.6 1,758,440 + Pf1N1B4_1527 0.66 -0.0 1,758,507 + Pf1N1B4_1527 0.76 -3.2 1,758,507 + Pf1N1B4_1527 0.76 -3.1 1,758,524 - Pf1N1B4_1527 0.79 -0.0 1,758,536 + Pf1N1B4_1527 0.80 -0.0 1,758,557 + Pf1N1B4_1527 0.83 -0.0 1,758,557 + Pf1N1B4_1527 0.83 -0.0 1,758,613 + -0.0 1,758,697 + -0.0 1,758,826 - -0.0 1,758,840 - -0.0 1,758,965 + -0.0 1,759,044 + -0.0 1,759,067 + -2.8 1,759,068 - -0.0 1,759,068 - -0.0 1,759,138 + -0.0 1,759,160 - -0.0 1,759,212 - -1.7 1,759,292 + -1.1 1,759,292 + -2.8 1,759,292 + -3.6 1,759,292 + -2.6 1,759,292 + -1.9 1,759,342 - -0.0 1,759,346 - -0.0 1,759,346 - -0.9 1,759,514 - -2.0
Or see this region's nucleotide sequence